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Improved cold tolerance in switchgrass by a novel CCCH-type zinc finger transcription factor gene, PvC3H72, associated with ICE1–CBF–COR regulon and ABA-responsive genes
BACKGROUND: Switchgrass (Panicum virgatum) is a warm-season perennial grass. Improving its cold tolerance is important for its sustainable production in cooler regions. Through genome-wide bioinformatic analysis of switchgrass Zinc finger-CCCH genes (PvC3Hs), we found that several PvC3Hs, including...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6753611/ https://www.ncbi.nlm.nih.gov/pubmed/31548866 http://dx.doi.org/10.1186/s13068-019-1564-y |
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author | Xie, Zheni Lin, Wenjing Yu, Guohui Cheng, Qiang Xu, Bin Huang, Bingru |
author_facet | Xie, Zheni Lin, Wenjing Yu, Guohui Cheng, Qiang Xu, Bin Huang, Bingru |
author_sort | Xie, Zheni |
collection | PubMed |
description | BACKGROUND: Switchgrass (Panicum virgatum) is a warm-season perennial grass. Improving its cold tolerance is important for its sustainable production in cooler regions. Through genome-wide bioinformatic analysis of switchgrass Zinc finger-CCCH genes (PvC3Hs), we found that several PvC3Hs, including PvC3H72, might play regulatory roles in plant cold tolerance. The objectives of this study were to characterize PvC3H72 using reverse genetics approach and to understand its functional role in cold signal transduction and cold tolerance in switchgrass. RESULTS: PvC3H72 is an intronless gene encoding a transcriptional activation factor. The expression of PvC3H72 was rapidly and highly induced by cold stress. Transgenic switchgrass with over-expressed PvC3H72 driven under maize ubiquitin promoter showed significantly improved chilling tolerance at 4 °C as demonstrated by less electrolyte leakage and higher relative water content than wild-type (WT) plants, as well as significantly higher survival rate after freezing treatment at − 5 °C. Improved cold tolerance of PvC3H72 transgenic lines was associated with significantly up-regulated expression of ICE1–CBF–COR regulon and ABA-responsive genes during cold treatment. CONCLUSIONS: PvC3H72 was the first characterized switchgrass cold-tolerance gene and also the only Znf-CCCH family gene known as a transcription factor in plant cold tolerance. PvC3H72 was an added signaling component in plant cold tolerance associated with regulation of ICE1–CBF–COR regulon and ABA-responsive genes. Knowledge gained in this study not only added another acting component into plant cold-tolerance mechanism, but also be of high value for genetic improvement of cold tolerance in switchgrass as well as other warm-season grasses. |
format | Online Article Text |
id | pubmed-6753611 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-67536112019-09-23 Improved cold tolerance in switchgrass by a novel CCCH-type zinc finger transcription factor gene, PvC3H72, associated with ICE1–CBF–COR regulon and ABA-responsive genes Xie, Zheni Lin, Wenjing Yu, Guohui Cheng, Qiang Xu, Bin Huang, Bingru Biotechnol Biofuels Research BACKGROUND: Switchgrass (Panicum virgatum) is a warm-season perennial grass. Improving its cold tolerance is important for its sustainable production in cooler regions. Through genome-wide bioinformatic analysis of switchgrass Zinc finger-CCCH genes (PvC3Hs), we found that several PvC3Hs, including PvC3H72, might play regulatory roles in plant cold tolerance. The objectives of this study were to characterize PvC3H72 using reverse genetics approach and to understand its functional role in cold signal transduction and cold tolerance in switchgrass. RESULTS: PvC3H72 is an intronless gene encoding a transcriptional activation factor. The expression of PvC3H72 was rapidly and highly induced by cold stress. Transgenic switchgrass with over-expressed PvC3H72 driven under maize ubiquitin promoter showed significantly improved chilling tolerance at 4 °C as demonstrated by less electrolyte leakage and higher relative water content than wild-type (WT) plants, as well as significantly higher survival rate after freezing treatment at − 5 °C. Improved cold tolerance of PvC3H72 transgenic lines was associated with significantly up-regulated expression of ICE1–CBF–COR regulon and ABA-responsive genes during cold treatment. CONCLUSIONS: PvC3H72 was the first characterized switchgrass cold-tolerance gene and also the only Znf-CCCH family gene known as a transcription factor in plant cold tolerance. PvC3H72 was an added signaling component in plant cold tolerance associated with regulation of ICE1–CBF–COR regulon and ABA-responsive genes. Knowledge gained in this study not only added another acting component into plant cold-tolerance mechanism, but also be of high value for genetic improvement of cold tolerance in switchgrass as well as other warm-season grasses. BioMed Central 2019-09-20 /pmc/articles/PMC6753611/ /pubmed/31548866 http://dx.doi.org/10.1186/s13068-019-1564-y Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Xie, Zheni Lin, Wenjing Yu, Guohui Cheng, Qiang Xu, Bin Huang, Bingru Improved cold tolerance in switchgrass by a novel CCCH-type zinc finger transcription factor gene, PvC3H72, associated with ICE1–CBF–COR regulon and ABA-responsive genes |
title | Improved cold tolerance in switchgrass by a novel CCCH-type zinc finger transcription factor gene, PvC3H72, associated with ICE1–CBF–COR regulon and ABA-responsive genes |
title_full | Improved cold tolerance in switchgrass by a novel CCCH-type zinc finger transcription factor gene, PvC3H72, associated with ICE1–CBF–COR regulon and ABA-responsive genes |
title_fullStr | Improved cold tolerance in switchgrass by a novel CCCH-type zinc finger transcription factor gene, PvC3H72, associated with ICE1–CBF–COR regulon and ABA-responsive genes |
title_full_unstemmed | Improved cold tolerance in switchgrass by a novel CCCH-type zinc finger transcription factor gene, PvC3H72, associated with ICE1–CBF–COR regulon and ABA-responsive genes |
title_short | Improved cold tolerance in switchgrass by a novel CCCH-type zinc finger transcription factor gene, PvC3H72, associated with ICE1–CBF–COR regulon and ABA-responsive genes |
title_sort | improved cold tolerance in switchgrass by a novel ccch-type zinc finger transcription factor gene, pvc3h72, associated with ice1–cbf–cor regulon and aba-responsive genes |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6753611/ https://www.ncbi.nlm.nih.gov/pubmed/31548866 http://dx.doi.org/10.1186/s13068-019-1564-y |
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