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Small RNA sequencing reveals microRNAs related to neuropathic pain in rats
The present study aimed to identify microRNAs (miRNAs) that are involved in neuropathic pain and predict their corresponding roles in the pathogenesis and development process of neuropathic pain. The rat model of neuropathic pain caused by spared nerve injury (SNI) was established in Sprague-Dawley...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Associação Brasileira de Divulgação Científica
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6753853/ https://www.ncbi.nlm.nih.gov/pubmed/31531524 http://dx.doi.org/10.1590/1414-431X20198380 |
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author | Dai, Dawei Wang, Junyu Jiang, Ying Yuan, Lei Lu, Youming Zhang, Aijun Zou, Dongdong Chen, Xin |
author_facet | Dai, Dawei Wang, Junyu Jiang, Ying Yuan, Lei Lu, Youming Zhang, Aijun Zou, Dongdong Chen, Xin |
author_sort | Dai, Dawei |
collection | PubMed |
description | The present study aimed to identify microRNAs (miRNAs) that are involved in neuropathic pain and predict their corresponding roles in the pathogenesis and development process of neuropathic pain. The rat model of neuropathic pain caused by spared nerve injury (SNI) was established in Sprague-Dawley male rats, followed by small RNA sequencing of the L3–L6 dorsal root ganglion. Real-time PCR was performed to validate the differently expressed miRNAs. Functional verification was performed by intrathecally injecting the animals with miRNA agomir. A total of 72 differentially expressed miRNAs were identified in the SNI rats, including 33 upregulated and 39 downregulated miRNAs. The results of qPCR further verified the expression levels of rno-miR-6215 (P=0.015), rno-miR-1224 (P=0.030), rno-miR-1249 (P=0.038), and rno-miR-488-3p (P=0.048), which were all significantly downregulated in the SNI rats compared to the control ones. The majority of differentially expressed miRNAs were associated with phosphorylation, intracellular signal transduction, and cell death. Target prediction, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses suggested that these differentially expressed miRNAs targeted genes that are related to axon guidance, focal adhesion, and Ras and Wnt signaling pathways. Moreover, miR-1224 agomir significantly alleviated SNI-induced neuropathic pain. The current findings provide new insights into the role of miRNAs in the pathogenesis of neuropathic pain. |
format | Online Article Text |
id | pubmed-6753853 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Associação Brasileira de Divulgação Científica |
record_format | MEDLINE/PubMed |
spelling | pubmed-67538532019-09-25 Small RNA sequencing reveals microRNAs related to neuropathic pain in rats Dai, Dawei Wang, Junyu Jiang, Ying Yuan, Lei Lu, Youming Zhang, Aijun Zou, Dongdong Chen, Xin Braz J Med Biol Res Research Article The present study aimed to identify microRNAs (miRNAs) that are involved in neuropathic pain and predict their corresponding roles in the pathogenesis and development process of neuropathic pain. The rat model of neuropathic pain caused by spared nerve injury (SNI) was established in Sprague-Dawley male rats, followed by small RNA sequencing of the L3–L6 dorsal root ganglion. Real-time PCR was performed to validate the differently expressed miRNAs. Functional verification was performed by intrathecally injecting the animals with miRNA agomir. A total of 72 differentially expressed miRNAs were identified in the SNI rats, including 33 upregulated and 39 downregulated miRNAs. The results of qPCR further verified the expression levels of rno-miR-6215 (P=0.015), rno-miR-1224 (P=0.030), rno-miR-1249 (P=0.038), and rno-miR-488-3p (P=0.048), which were all significantly downregulated in the SNI rats compared to the control ones. The majority of differentially expressed miRNAs were associated with phosphorylation, intracellular signal transduction, and cell death. Target prediction, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses suggested that these differentially expressed miRNAs targeted genes that are related to axon guidance, focal adhesion, and Ras and Wnt signaling pathways. Moreover, miR-1224 agomir significantly alleviated SNI-induced neuropathic pain. The current findings provide new insights into the role of miRNAs in the pathogenesis of neuropathic pain. Associação Brasileira de Divulgação Científica 2019-09-16 /pmc/articles/PMC6753853/ /pubmed/31531524 http://dx.doi.org/10.1590/1414-431X20198380 Text en https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Dai, Dawei Wang, Junyu Jiang, Ying Yuan, Lei Lu, Youming Zhang, Aijun Zou, Dongdong Chen, Xin Small RNA sequencing reveals microRNAs related to neuropathic pain in rats |
title | Small RNA sequencing reveals microRNAs related to neuropathic pain in rats |
title_full | Small RNA sequencing reveals microRNAs related to neuropathic pain in rats |
title_fullStr | Small RNA sequencing reveals microRNAs related to neuropathic pain in rats |
title_full_unstemmed | Small RNA sequencing reveals microRNAs related to neuropathic pain in rats |
title_short | Small RNA sequencing reveals microRNAs related to neuropathic pain in rats |
title_sort | small rna sequencing reveals micrornas related to neuropathic pain in rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6753853/ https://www.ncbi.nlm.nih.gov/pubmed/31531524 http://dx.doi.org/10.1590/1414-431X20198380 |
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