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TprA/PhrA Quorum Sensing System Has a Major Effect on Pneumococcal Survival in Respiratory Tract and Blood, and Its Activity Is Controlled by CcpA and GlnR

Streptococcus pneumoniae is able to cause deadly diseases by infecting different tissues, each with distinct environmental and nutritional compositions. We hypothesize that the adaptive capabilities of the microbe is an important facet of pneumococcal survival in fluctuating host environments. Quoru...

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Autores principales: Motib, Anfal Shakir, Al-Bayati, Firas A. Y., Manzoor, Irfan, Shafeeq, Sulman, Kadam, Anagha, Kuipers, Oscar P., Hiller, N. Luisa, Andrew, Peter W., Yesilkaya, Hasan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6753895/
https://www.ncbi.nlm.nih.gov/pubmed/31572692
http://dx.doi.org/10.3389/fcimb.2019.00326
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author Motib, Anfal Shakir
Al-Bayati, Firas A. Y.
Manzoor, Irfan
Shafeeq, Sulman
Kadam, Anagha
Kuipers, Oscar P.
Hiller, N. Luisa
Andrew, Peter W.
Yesilkaya, Hasan
author_facet Motib, Anfal Shakir
Al-Bayati, Firas A. Y.
Manzoor, Irfan
Shafeeq, Sulman
Kadam, Anagha
Kuipers, Oscar P.
Hiller, N. Luisa
Andrew, Peter W.
Yesilkaya, Hasan
author_sort Motib, Anfal Shakir
collection PubMed
description Streptococcus pneumoniae is able to cause deadly diseases by infecting different tissues, each with distinct environmental and nutritional compositions. We hypothesize that the adaptive capabilities of the microbe is an important facet of pneumococcal survival in fluctuating host environments. Quorum-sensing (QS) mechanisms are pivotal for microbial host adaptation. We previously demonstrated that the TprA/PhrA QS system is required for pneumococcal utilization of galactose and mannose, neuraminidase activity, and virulence. We also showed that the system can be modulated by using linear molecularly imprinted polymers. Due to being a drugable target, we further studied the operation of this QS system in S. pneumoniae. We found that TprA controls the expression of nine different operons on galactose and mannose. Our data revealed that TprA expression is modulated by a complex regulatory network, where the master regulators CcpA and GlnR are involved in a sugar dependent manner. Mutants in the TprA/PhrA system are highly attenuated in their survival in nasopharynx and lungs after intranasal infection, and growth in blood after intravenous infection.
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spelling pubmed-67538952019-09-30 TprA/PhrA Quorum Sensing System Has a Major Effect on Pneumococcal Survival in Respiratory Tract and Blood, and Its Activity Is Controlled by CcpA and GlnR Motib, Anfal Shakir Al-Bayati, Firas A. Y. Manzoor, Irfan Shafeeq, Sulman Kadam, Anagha Kuipers, Oscar P. Hiller, N. Luisa Andrew, Peter W. Yesilkaya, Hasan Front Cell Infect Microbiol Cellular and Infection Microbiology Streptococcus pneumoniae is able to cause deadly diseases by infecting different tissues, each with distinct environmental and nutritional compositions. We hypothesize that the adaptive capabilities of the microbe is an important facet of pneumococcal survival in fluctuating host environments. Quorum-sensing (QS) mechanisms are pivotal for microbial host adaptation. We previously demonstrated that the TprA/PhrA QS system is required for pneumococcal utilization of galactose and mannose, neuraminidase activity, and virulence. We also showed that the system can be modulated by using linear molecularly imprinted polymers. Due to being a drugable target, we further studied the operation of this QS system in S. pneumoniae. We found that TprA controls the expression of nine different operons on galactose and mannose. Our data revealed that TprA expression is modulated by a complex regulatory network, where the master regulators CcpA and GlnR are involved in a sugar dependent manner. Mutants in the TprA/PhrA system are highly attenuated in their survival in nasopharynx and lungs after intranasal infection, and growth in blood after intravenous infection. Frontiers Media S.A. 2019-09-13 /pmc/articles/PMC6753895/ /pubmed/31572692 http://dx.doi.org/10.3389/fcimb.2019.00326 Text en Copyright © 2019 Motib, Al-Bayati, Manzoor, Shafeeq, Kadam, Kuipers, Hiller, Andrew and Yesilkaya. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Motib, Anfal Shakir
Al-Bayati, Firas A. Y.
Manzoor, Irfan
Shafeeq, Sulman
Kadam, Anagha
Kuipers, Oscar P.
Hiller, N. Luisa
Andrew, Peter W.
Yesilkaya, Hasan
TprA/PhrA Quorum Sensing System Has a Major Effect on Pneumococcal Survival in Respiratory Tract and Blood, and Its Activity Is Controlled by CcpA and GlnR
title TprA/PhrA Quorum Sensing System Has a Major Effect on Pneumococcal Survival in Respiratory Tract and Blood, and Its Activity Is Controlled by CcpA and GlnR
title_full TprA/PhrA Quorum Sensing System Has a Major Effect on Pneumococcal Survival in Respiratory Tract and Blood, and Its Activity Is Controlled by CcpA and GlnR
title_fullStr TprA/PhrA Quorum Sensing System Has a Major Effect on Pneumococcal Survival in Respiratory Tract and Blood, and Its Activity Is Controlled by CcpA and GlnR
title_full_unstemmed TprA/PhrA Quorum Sensing System Has a Major Effect on Pneumococcal Survival in Respiratory Tract and Blood, and Its Activity Is Controlled by CcpA and GlnR
title_short TprA/PhrA Quorum Sensing System Has a Major Effect on Pneumococcal Survival in Respiratory Tract and Blood, and Its Activity Is Controlled by CcpA and GlnR
title_sort tpra/phra quorum sensing system has a major effect on pneumococcal survival in respiratory tract and blood, and its activity is controlled by ccpa and glnr
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6753895/
https://www.ncbi.nlm.nih.gov/pubmed/31572692
http://dx.doi.org/10.3389/fcimb.2019.00326
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