Enhanced CRISPR-based DNA demethylation by Casilio-ME-mediated RNA-guided coupling of methylcytosine oxidation and DNA repair pathways

Here we develop a methylation editing toolbox, Casilio-ME, that enables not only RNA-guided methylcytosine editing by targeting TET1 to genomic sites, but also by co-delivering TET1 and protein factors that couple methylcytosine oxidation to DNA repair activities, and/or promote TET1 to achieve enha...

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Detalles Bibliográficos
Autores principales: Taghbalout, Aziz, Du, Menghan, Jillette, Nathaniel, Rosikiewicz, Wojciech, Rath, Abhijit, Heinen, Christopher D., Li, Sheng, Cheng, Albert W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6754513/
https://www.ncbi.nlm.nih.gov/pubmed/31541098
http://dx.doi.org/10.1038/s41467-019-12339-7

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