Application of GLAD-PCR Assay for Study on DNA Methylation in Regulatory Regions of Some Tumor-Suppressor Genes in Lung Cancer

Hypermethylation of the gene regulatory regions are common for many cancer diseases. In this work we applied GLAD-PCR assay for identificating of the aberrantly methylated RCGY sites in the regulatory regions of some downregulated genes in tissue samples of lung cancer (LC). This list includes EFEMP...

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Autores principales: Smetannikova, N.A., Evdokimov, A.A., Netesova, N.A., Abdurashitov, M.A., Akishev, A.G., Dubinin, E.V., Pozdnyakov, P.I., Vihlyanov, I.V., Nikitin, M.K., Topolnitsky, E.B., Karpov, A.B., Kolomiets, S.A., Degtyarev, S.Kh.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 中国肺癌杂志编辑部 2019
Materias:
Basic Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6754573/
https://www.ncbi.nlm.nih.gov/pubmed/31526458
http://dx.doi.org/10.3779/j.issn.1009-3419.2019.09.01
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author Smetannikova, N.A.
Evdokimov, A.A.
Netesova, N.A.
Abdurashitov, M.A.
Akishev, A.G.
Dubinin, E.V.
Pozdnyakov, P.I.
Vihlyanov, I.V.
Nikitin, M.K.
Topolnitsky, E.B.
Karpov, A.B.
Kolomiets, S.A.
Degtyarev, S.Kh.
author_facet Smetannikova, N.A.
Evdokimov, A.A.
Netesova, N.A.
Abdurashitov, M.A.
Akishev, A.G.
Dubinin, E.V.
Pozdnyakov, P.I.
Vihlyanov, I.V.
Nikitin, M.K.
Topolnitsky, E.B.
Karpov, A.B.
Kolomiets, S.A.
Degtyarev, S.Kh.
author_sort Smetannikova, N.A.
collection PubMed
description Hypermethylation of the gene regulatory regions are common for many cancer diseases. In this work we applied GLAD-PCR assay for identificating of the aberrantly methylated RCGY sites in the regulatory regions of some downregulated genes in tissue samples of lung cancer (LC). This list includes EFEMP1, EPHA5, HOXA5, HOXA9, LHX1, MYF6, NID2, OTX1, PAX9, RARB, RASSF1A, RXRG, SIX6, SKOR1 and TERT genes. The results of DNA samples from 40 cancer and 25 normal lung tissues showed a good diagnostic potential of selected RCGY sites in regulatory regions of MYF6, SIX6, RXRG, LHX1, RASSF1A and TERT genes with relatively high sensitivity (80.0 %) and specificity (88.0 %) of LC detection in tumor DNA.
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spelling pubmed-67545732019-10-07 Application of GLAD-PCR Assay for Study on DNA Methylation in Regulatory Regions of Some Tumor-Suppressor Genes in Lung Cancer Smetannikova, N.A. Evdokimov, A.A. Netesova, N.A. Abdurashitov, M.A. Akishev, A.G. Dubinin, E.V. Pozdnyakov, P.I. Vihlyanov, I.V. Nikitin, M.K. Topolnitsky, E.B. Karpov, A.B. Kolomiets, S.A. Degtyarev, S.Kh. Zhongguo Fei Ai Za Zhi Basic Research Hypermethylation of the gene regulatory regions are common for many cancer diseases. In this work we applied GLAD-PCR assay for identificating of the aberrantly methylated RCGY sites in the regulatory regions of some downregulated genes in tissue samples of lung cancer (LC). This list includes EFEMP1, EPHA5, HOXA5, HOXA9, LHX1, MYF6, NID2, OTX1, PAX9, RARB, RASSF1A, RXRG, SIX6, SKOR1 and TERT genes. The results of DNA samples from 40 cancer and 25 normal lung tissues showed a good diagnostic potential of selected RCGY sites in regulatory regions of MYF6, SIX6, RXRG, LHX1, RASSF1A and TERT genes with relatively high sensitivity (80.0 %) and specificity (88.0 %) of LC detection in tumor DNA. 中国肺癌杂志编辑部 2019-09-20 /pmc/articles/PMC6754573/ /pubmed/31526458 http://dx.doi.org/10.3779/j.issn.1009-3419.2019.09.01 Text en 版权所有©《中国肺癌杂志》编辑部2019 https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed in accordance with the terms of the Creative Commons Attribution (CC BY 3.0) License. See: https://creativecommons.org/licenses/by/3.0/
spellingShingle Basic Research
Smetannikova, N.A.
Evdokimov, A.A.
Netesova, N.A.
Abdurashitov, M.A.
Akishev, A.G.
Dubinin, E.V.
Pozdnyakov, P.I.
Vihlyanov, I.V.
Nikitin, M.K.
Topolnitsky, E.B.
Karpov, A.B.
Kolomiets, S.A.
Degtyarev, S.Kh.
Application of GLAD-PCR Assay for Study on DNA Methylation in Regulatory Regions of Some Tumor-Suppressor Genes in Lung Cancer
title Application of GLAD-PCR Assay for Study on DNA Methylation in Regulatory Regions of Some Tumor-Suppressor Genes in Lung Cancer
title_full Application of GLAD-PCR Assay for Study on DNA Methylation in Regulatory Regions of Some Tumor-Suppressor Genes in Lung Cancer
title_fullStr Application of GLAD-PCR Assay for Study on DNA Methylation in Regulatory Regions of Some Tumor-Suppressor Genes in Lung Cancer
title_full_unstemmed Application of GLAD-PCR Assay for Study on DNA Methylation in Regulatory Regions of Some Tumor-Suppressor Genes in Lung Cancer
title_short Application of GLAD-PCR Assay for Study on DNA Methylation in Regulatory Regions of Some Tumor-Suppressor Genes in Lung Cancer
title_sort application of glad-pcr assay for study on dna methylation in regulatory regions of some tumor-suppressor genes in lung cancer
topic Basic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6754573/
https://www.ncbi.nlm.nih.gov/pubmed/31526458
http://dx.doi.org/10.3779/j.issn.1009-3419.2019.09.01
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