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Mismatches between the genetic and phenotypic sex in the wild Kou population of Nile tilapia Oreochromis niloticus
Sex determination and sex chromosomes can be very diverse between teleost species. The group of tilapias shows a polymorphism in sex determination not only between closely related species but also between domestic strains within a species. In the Nile tilapia, the major effect genes and therefore th...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6754722/ https://www.ncbi.nlm.nih.gov/pubmed/31579600 http://dx.doi.org/10.7717/peerj.7709 |
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author | Sissao, Rokyatou D’Cotta, Helena Baroiller, Jean-François Toguyeni, Aboubacar |
author_facet | Sissao, Rokyatou D’Cotta, Helena Baroiller, Jean-François Toguyeni, Aboubacar |
author_sort | Sissao, Rokyatou |
collection | PubMed |
description | Sex determination and sex chromosomes can be very diverse between teleost species. The group of tilapias shows a polymorphism in sex determination not only between closely related species but also between domestic strains within a species. In the Nile tilapia, the major effect genes and therefore the Y chromosome have been located on either linkage group 1 (LG1) or LG23 depending on the strains. In a Japanese strain, the sex determinant of LG23 (the amhY gene) has been identified as a duplicated amh (anti-Müllerian hormone) gene, with its gametolog found on the X chromosome (amhX). AmhY is located in tandem with the amhΔY gene (a truncated form) on the Y chromosome. X and Y chromosome markers based on the amh genes have been validated only on a few domestic strains but not in wild populations. Here, we used four of these markers in order to examine (1) the possible variation in sex determination of a wild population of Nile tilapia living in Lake Kou (Burkina Faso), (2) putative polymorphisms for these amh copies and (3) the existence of sex reversed individuals in the wild. Our genotyping of 91 wild Kou individuals with the amh sex-diagnostic markers of LG23 showed that while phenotypic females were all XX, phenotypic males were either XY or XX. Progeny testing of eight of these XX males revealed that one of these males consistently sired all-female progenies, suggesting that it is a wild sex reversed male (which could result from high temperature effects). The other XX males gave balanced sex ratios, suggesting that sex is controlled by another locus (possibly on another LG) which may be epistatically dominant over the LG23 locus. Finally, identification of unexpected amh genotypes was found for two individuals. They produced either balanced or female-biased sex ratios, depending on the breeder with whom they were crossed, suggesting possible recombination between the X and the Y chromosomes. |
format | Online Article Text |
id | pubmed-6754722 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-67547222019-10-02 Mismatches between the genetic and phenotypic sex in the wild Kou population of Nile tilapia Oreochromis niloticus Sissao, Rokyatou D’Cotta, Helena Baroiller, Jean-François Toguyeni, Aboubacar PeerJ Aquaculture, Fisheries and Fish Science Sex determination and sex chromosomes can be very diverse between teleost species. The group of tilapias shows a polymorphism in sex determination not only between closely related species but also between domestic strains within a species. In the Nile tilapia, the major effect genes and therefore the Y chromosome have been located on either linkage group 1 (LG1) or LG23 depending on the strains. In a Japanese strain, the sex determinant of LG23 (the amhY gene) has been identified as a duplicated amh (anti-Müllerian hormone) gene, with its gametolog found on the X chromosome (amhX). AmhY is located in tandem with the amhΔY gene (a truncated form) on the Y chromosome. X and Y chromosome markers based on the amh genes have been validated only on a few domestic strains but not in wild populations. Here, we used four of these markers in order to examine (1) the possible variation in sex determination of a wild population of Nile tilapia living in Lake Kou (Burkina Faso), (2) putative polymorphisms for these amh copies and (3) the existence of sex reversed individuals in the wild. Our genotyping of 91 wild Kou individuals with the amh sex-diagnostic markers of LG23 showed that while phenotypic females were all XX, phenotypic males were either XY or XX. Progeny testing of eight of these XX males revealed that one of these males consistently sired all-female progenies, suggesting that it is a wild sex reversed male (which could result from high temperature effects). The other XX males gave balanced sex ratios, suggesting that sex is controlled by another locus (possibly on another LG) which may be epistatically dominant over the LG23 locus. Finally, identification of unexpected amh genotypes was found for two individuals. They produced either balanced or female-biased sex ratios, depending on the breeder with whom they were crossed, suggesting possible recombination between the X and the Y chromosomes. PeerJ Inc. 2019-09-18 /pmc/articles/PMC6754722/ /pubmed/31579600 http://dx.doi.org/10.7717/peerj.7709 Text en ©2019 Sissao et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Aquaculture, Fisheries and Fish Science Sissao, Rokyatou D’Cotta, Helena Baroiller, Jean-François Toguyeni, Aboubacar Mismatches between the genetic and phenotypic sex in the wild Kou population of Nile tilapia Oreochromis niloticus |
title | Mismatches between the genetic and phenotypic sex in the wild Kou population of Nile tilapia Oreochromis niloticus |
title_full | Mismatches between the genetic and phenotypic sex in the wild Kou population of Nile tilapia Oreochromis niloticus |
title_fullStr | Mismatches between the genetic and phenotypic sex in the wild Kou population of Nile tilapia Oreochromis niloticus |
title_full_unstemmed | Mismatches between the genetic and phenotypic sex in the wild Kou population of Nile tilapia Oreochromis niloticus |
title_short | Mismatches between the genetic and phenotypic sex in the wild Kou population of Nile tilapia Oreochromis niloticus |
title_sort | mismatches between the genetic and phenotypic sex in the wild kou population of nile tilapia oreochromis niloticus |
topic | Aquaculture, Fisheries and Fish Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6754722/ https://www.ncbi.nlm.nih.gov/pubmed/31579600 http://dx.doi.org/10.7717/peerj.7709 |
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