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Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time
A capacitive sensor was developed to analyze the presence and enzymatic activity of a model protease from standard solutions by following the degradation of the substrate in real time. The enzyme was chosen based on its specific digestion of the hinge region of immunoglobulin G (IgG). Real-time enzy...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6754820/ https://www.ncbi.nlm.nih.gov/pubmed/31020512 http://dx.doi.org/10.1007/s12010-019-03006-0 |
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author | Bergdahl, Gizem Ertürk Hedström, Martin Mattiasson, Bo |
author_facet | Bergdahl, Gizem Ertürk Hedström, Martin Mattiasson, Bo |
author_sort | Bergdahl, Gizem Ertürk |
collection | PubMed |
description | A capacitive sensor was developed to analyze the presence and enzymatic activity of a model protease from standard solutions by following the degradation of the substrate in real time. The enzyme was chosen based on its specific digestion of the hinge region of immunoglobulin G (IgG). Real-time enzyme activity was monitored by measuring the change in capacitance (∆C) based on the release of IgG fragments after enzymatic digestion by the enzyme. The results indicated that the developed capacitive system might be used successfully for label-free and real-time monitoring of enzymatic activity of different enzymes in a sensitive, rapid, and inexpensive manner in biotechnological, environmental, and clinical applications. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s12010-019-03006-0) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6754820 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-67548202019-10-04 Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time Bergdahl, Gizem Ertürk Hedström, Martin Mattiasson, Bo Appl Biochem Biotechnol Article A capacitive sensor was developed to analyze the presence and enzymatic activity of a model protease from standard solutions by following the degradation of the substrate in real time. The enzyme was chosen based on its specific digestion of the hinge region of immunoglobulin G (IgG). Real-time enzyme activity was monitored by measuring the change in capacitance (∆C) based on the release of IgG fragments after enzymatic digestion by the enzyme. The results indicated that the developed capacitive system might be used successfully for label-free and real-time monitoring of enzymatic activity of different enzymes in a sensitive, rapid, and inexpensive manner in biotechnological, environmental, and clinical applications. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s12010-019-03006-0) contains supplementary material, which is available to authorized users. Springer US 2019-04-24 2019 /pmc/articles/PMC6754820/ /pubmed/31020512 http://dx.doi.org/10.1007/s12010-019-03006-0 Text en © The Author(s) 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Article Bergdahl, Gizem Ertürk Hedström, Martin Mattiasson, Bo Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time |
title | Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time |
title_full | Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time |
title_fullStr | Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time |
title_full_unstemmed | Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time |
title_short | Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time |
title_sort | capacitive sensor to monitor enzyme activity by following degradation of macromolecules in real time |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6754820/ https://www.ncbi.nlm.nih.gov/pubmed/31020512 http://dx.doi.org/10.1007/s12010-019-03006-0 |
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