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Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time

A capacitive sensor was developed to analyze the presence and enzymatic activity of a model protease from standard solutions by following the degradation of the substrate in real time. The enzyme was chosen based on its specific digestion of the hinge region of immunoglobulin G (IgG). Real-time enzy...

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Detalles Bibliográficos
Autores principales: Bergdahl, Gizem Ertürk, Hedström, Martin, Mattiasson, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6754820/
https://www.ncbi.nlm.nih.gov/pubmed/31020512
http://dx.doi.org/10.1007/s12010-019-03006-0
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author Bergdahl, Gizem Ertürk
Hedström, Martin
Mattiasson, Bo
author_facet Bergdahl, Gizem Ertürk
Hedström, Martin
Mattiasson, Bo
author_sort Bergdahl, Gizem Ertürk
collection PubMed
description A capacitive sensor was developed to analyze the presence and enzymatic activity of a model protease from standard solutions by following the degradation of the substrate in real time. The enzyme was chosen based on its specific digestion of the hinge region of immunoglobulin G (IgG). Real-time enzyme activity was monitored by measuring the change in capacitance (∆C) based on the release of IgG fragments after enzymatic digestion by the enzyme. The results indicated that the developed capacitive system might be used successfully for label-free and real-time monitoring of enzymatic activity of different enzymes in a sensitive, rapid, and inexpensive manner in biotechnological, environmental, and clinical applications. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s12010-019-03006-0) contains supplementary material, which is available to authorized users.
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spelling pubmed-67548202019-10-04 Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time Bergdahl, Gizem Ertürk Hedström, Martin Mattiasson, Bo Appl Biochem Biotechnol Article A capacitive sensor was developed to analyze the presence and enzymatic activity of a model protease from standard solutions by following the degradation of the substrate in real time. The enzyme was chosen based on its specific digestion of the hinge region of immunoglobulin G (IgG). Real-time enzyme activity was monitored by measuring the change in capacitance (∆C) based on the release of IgG fragments after enzymatic digestion by the enzyme. The results indicated that the developed capacitive system might be used successfully for label-free and real-time monitoring of enzymatic activity of different enzymes in a sensitive, rapid, and inexpensive manner in biotechnological, environmental, and clinical applications. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s12010-019-03006-0) contains supplementary material, which is available to authorized users. Springer US 2019-04-24 2019 /pmc/articles/PMC6754820/ /pubmed/31020512 http://dx.doi.org/10.1007/s12010-019-03006-0 Text en © The Author(s) 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Article
Bergdahl, Gizem Ertürk
Hedström, Martin
Mattiasson, Bo
Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time
title Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time
title_full Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time
title_fullStr Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time
title_full_unstemmed Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time
title_short Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time
title_sort capacitive sensor to monitor enzyme activity by following degradation of macromolecules in real time
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6754820/
https://www.ncbi.nlm.nih.gov/pubmed/31020512
http://dx.doi.org/10.1007/s12010-019-03006-0
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