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Cloning and characterization of the lignin biosynthesis genes NcCSE and NcHCT from Neolamarckia cadamba
Neolamarckia cadamba is an important fast growing tree species used for pulping and wood material in industry for it’s desirable wood properties. As one of the most important content in wood, lignin provides structural integrity, strength, and hydrophobicity to the thickened cell walls and is the ma...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6754823/ https://www.ncbi.nlm.nih.gov/pubmed/31542835 http://dx.doi.org/10.1186/s13568-019-0860-z |
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author | Li, Juncheng Huang, Xiaoling Huang, Hao Huo, Heqiang Nguyen, Chi D. Pian, Ruiqi Li, Huaqiang Ouyang, Kunxi Chen, Xiaoyang |
author_facet | Li, Juncheng Huang, Xiaoling Huang, Hao Huo, Heqiang Nguyen, Chi D. Pian, Ruiqi Li, Huaqiang Ouyang, Kunxi Chen, Xiaoyang |
author_sort | Li, Juncheng |
collection | PubMed |
description | Neolamarckia cadamba is an important fast growing tree species used for pulping and wood material in industry for it’s desirable wood properties. As one of the most important content in wood, lignin provides structural integrity, strength, and hydrophobicity to the thickened cell walls and is the major factor contributing to biomass recalcitrance. It does not reduce the palatability of forage grass for animals, but it hinders the isolation of cellulose fibers and the efficient enzymatic depolymerization of cellulose and hemicellulose into fermentable sugars in biorefining processes by limiting the access by hydrolytic enzymes to their polysaccharide substrates. This work focused on analyzing the functions of NcCSE (Caffeoyl Shikimate Esterase, GenBank accession number: MG739672) and NcHCT (Hydroxycinnamoyl Transferase,GenBank accession number: MG739673) in the lignin biosynthetic process in order to improve the potential for utilization of leaves and wood from N. cadamba. The mutant phenotype of cse-2 was dramatically complemented to WT in the stable transgenic lines cse-35S::NcCSE, but overexpression of NcHCT in the cse-2 mutant did not have the same result as cse-35S::NcCSE, providing only partial complementation. |
format | Online Article Text |
id | pubmed-6754823 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-67548232019-10-04 Cloning and characterization of the lignin biosynthesis genes NcCSE and NcHCT from Neolamarckia cadamba Li, Juncheng Huang, Xiaoling Huang, Hao Huo, Heqiang Nguyen, Chi D. Pian, Ruiqi Li, Huaqiang Ouyang, Kunxi Chen, Xiaoyang AMB Express Original Article Neolamarckia cadamba is an important fast growing tree species used for pulping and wood material in industry for it’s desirable wood properties. As one of the most important content in wood, lignin provides structural integrity, strength, and hydrophobicity to the thickened cell walls and is the major factor contributing to biomass recalcitrance. It does not reduce the palatability of forage grass for animals, but it hinders the isolation of cellulose fibers and the efficient enzymatic depolymerization of cellulose and hemicellulose into fermentable sugars in biorefining processes by limiting the access by hydrolytic enzymes to their polysaccharide substrates. This work focused on analyzing the functions of NcCSE (Caffeoyl Shikimate Esterase, GenBank accession number: MG739672) and NcHCT (Hydroxycinnamoyl Transferase,GenBank accession number: MG739673) in the lignin biosynthetic process in order to improve the potential for utilization of leaves and wood from N. cadamba. The mutant phenotype of cse-2 was dramatically complemented to WT in the stable transgenic lines cse-35S::NcCSE, but overexpression of NcHCT in the cse-2 mutant did not have the same result as cse-35S::NcCSE, providing only partial complementation. Springer Berlin Heidelberg 2019-09-21 /pmc/articles/PMC6754823/ /pubmed/31542835 http://dx.doi.org/10.1186/s13568-019-0860-z Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Li, Juncheng Huang, Xiaoling Huang, Hao Huo, Heqiang Nguyen, Chi D. Pian, Ruiqi Li, Huaqiang Ouyang, Kunxi Chen, Xiaoyang Cloning and characterization of the lignin biosynthesis genes NcCSE and NcHCT from Neolamarckia cadamba |
title | Cloning and characterization of the lignin biosynthesis genes NcCSE and NcHCT from Neolamarckia cadamba |
title_full | Cloning and characterization of the lignin biosynthesis genes NcCSE and NcHCT from Neolamarckia cadamba |
title_fullStr | Cloning and characterization of the lignin biosynthesis genes NcCSE and NcHCT from Neolamarckia cadamba |
title_full_unstemmed | Cloning and characterization of the lignin biosynthesis genes NcCSE and NcHCT from Neolamarckia cadamba |
title_short | Cloning and characterization of the lignin biosynthesis genes NcCSE and NcHCT from Neolamarckia cadamba |
title_sort | cloning and characterization of the lignin biosynthesis genes nccse and nchct from neolamarckia cadamba |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6754823/ https://www.ncbi.nlm.nih.gov/pubmed/31542835 http://dx.doi.org/10.1186/s13568-019-0860-z |
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