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Antioxidant and Hypolipidemic Activities of Acid-Depolymerised Exopolysaccharides by Termitomyces albuminosus

The acid-depolymerised exopolysaccharides (ADES) of Termitomyces albuminosus were obtained, and the major fraction of ADES1 was isolated and purified by DEAE-52 cellulose anion-exchange column chromatography. Physicochemical characterizations showed that ADES1 was an α- and a β-configuration with th...

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Autores principales: Zhao, Huajie, Wang, Xiuxiu, Liu, Xinchao, Zhang, Jianjun, Wan, Luzhang, Jia, Le
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6754963/
https://www.ncbi.nlm.nih.gov/pubmed/31583046
http://dx.doi.org/10.1155/2019/8915272
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author Zhao, Huajie
Wang, Xiuxiu
Liu, Xinchao
Zhang, Jianjun
Wan, Luzhang
Jia, Le
author_facet Zhao, Huajie
Wang, Xiuxiu
Liu, Xinchao
Zhang, Jianjun
Wan, Luzhang
Jia, Le
author_sort Zhao, Huajie
collection PubMed
description The acid-depolymerised exopolysaccharides (ADES) of Termitomyces albuminosus were obtained, and the major fraction of ADES1 was isolated and purified by DEAE-52 cellulose anion-exchange column chromatography. Physicochemical characterizations showed that ADES1 was an α- and a β-configuration with the molecular weight of 2.43 kDa, containing (1→3, 4)-linked-Glcp, (1→4)-linked-D-Glcp, (1→3)-linked-D-Xylp, (1→4)-linked-D-Manp, T-Glcp, (1→6)-linked-D-Galp, and (1→4)-linked-L-Arap. The in vivo assays showed that ADES1 could reduce lipid levels in the serum and liver, decrease serum enzyme activities, and improve antioxidant enzyme activities and p-AMPKα expressions in hyperlipidemic mice, which were also confirmed by histopathological observations. These data indicated that ADES1 might be considered as a novel substance to treat and prevent hyperlipidemia and as a hepatoprotective agent.
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spelling pubmed-67549632019-10-03 Antioxidant and Hypolipidemic Activities of Acid-Depolymerised Exopolysaccharides by Termitomyces albuminosus Zhao, Huajie Wang, Xiuxiu Liu, Xinchao Zhang, Jianjun Wan, Luzhang Jia, Le Oxid Med Cell Longev Research Article The acid-depolymerised exopolysaccharides (ADES) of Termitomyces albuminosus were obtained, and the major fraction of ADES1 was isolated and purified by DEAE-52 cellulose anion-exchange column chromatography. Physicochemical characterizations showed that ADES1 was an α- and a β-configuration with the molecular weight of 2.43 kDa, containing (1→3, 4)-linked-Glcp, (1→4)-linked-D-Glcp, (1→3)-linked-D-Xylp, (1→4)-linked-D-Manp, T-Glcp, (1→6)-linked-D-Galp, and (1→4)-linked-L-Arap. The in vivo assays showed that ADES1 could reduce lipid levels in the serum and liver, decrease serum enzyme activities, and improve antioxidant enzyme activities and p-AMPKα expressions in hyperlipidemic mice, which were also confirmed by histopathological observations. These data indicated that ADES1 might be considered as a novel substance to treat and prevent hyperlipidemia and as a hepatoprotective agent. Hindawi 2019-09-08 /pmc/articles/PMC6754963/ /pubmed/31583046 http://dx.doi.org/10.1155/2019/8915272 Text en Copyright © 2019 Huajie Zhao et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhao, Huajie
Wang, Xiuxiu
Liu, Xinchao
Zhang, Jianjun
Wan, Luzhang
Jia, Le
Antioxidant and Hypolipidemic Activities of Acid-Depolymerised Exopolysaccharides by Termitomyces albuminosus
title Antioxidant and Hypolipidemic Activities of Acid-Depolymerised Exopolysaccharides by Termitomyces albuminosus
title_full Antioxidant and Hypolipidemic Activities of Acid-Depolymerised Exopolysaccharides by Termitomyces albuminosus
title_fullStr Antioxidant and Hypolipidemic Activities of Acid-Depolymerised Exopolysaccharides by Termitomyces albuminosus
title_full_unstemmed Antioxidant and Hypolipidemic Activities of Acid-Depolymerised Exopolysaccharides by Termitomyces albuminosus
title_short Antioxidant and Hypolipidemic Activities of Acid-Depolymerised Exopolysaccharides by Termitomyces albuminosus
title_sort antioxidant and hypolipidemic activities of acid-depolymerised exopolysaccharides by termitomyces albuminosus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6754963/
https://www.ncbi.nlm.nih.gov/pubmed/31583046
http://dx.doi.org/10.1155/2019/8915272
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