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TrmB, a tRNA m(7)G46 methyltransferase, plays a role in hydrogen peroxide resistance and positively modulates the translation of katA and katB mRNAs in Pseudomonas aeruginosa
Cellular response to oxidative stress is a crucial mechanism that promotes the survival of Pseudomonas aeruginosa during infection. However, the translational regulation of oxidative stress response remains largely unknown. Here, we reveal a tRNA modification-mediated translational response to H(2)O...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6755087/ https://www.ncbi.nlm.nih.gov/pubmed/31428787 http://dx.doi.org/10.1093/nar/gkz702 |
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author | Thongdee, Narumon Jaroensuk, Juthamas Atichartpongkul, Sopapan Chittrakanwong, Jurairat Chooyoung, Kamonchanok Srimahaeak, Thanyaporn Chaiyen, Pimchai Vattanaviboon, Paiboon Mongkolsuk, Skorn Fuangthong, Mayuree |
author_facet | Thongdee, Narumon Jaroensuk, Juthamas Atichartpongkul, Sopapan Chittrakanwong, Jurairat Chooyoung, Kamonchanok Srimahaeak, Thanyaporn Chaiyen, Pimchai Vattanaviboon, Paiboon Mongkolsuk, Skorn Fuangthong, Mayuree |
author_sort | Thongdee, Narumon |
collection | PubMed |
description | Cellular response to oxidative stress is a crucial mechanism that promotes the survival of Pseudomonas aeruginosa during infection. However, the translational regulation of oxidative stress response remains largely unknown. Here, we reveal a tRNA modification-mediated translational response to H(2)O(2) in P. aeruginosa. We demonstrated that the P. aeruginosa trmB gene encodes a tRNA guanine (46)-N7-methyltransferase that catalyzes the formation of m(7)G46 in the tRNA variable loop. Twenty-three tRNA substrates of TrmB with a guanosine residue at position 46 were identified, including 11 novel tRNA substrates. We showed that loss of trmB had a strong negative effect on the translation of Phe- and Asp-enriched mRNAs. The trmB-mediated m(7)G modification modulated the expression of the catalase genes katA and katB, which are enriched with Phe/Asp codons at the translational level. In response to H(2)O(2) exposure, the level of m(7)G modification increased, consistent with the increased translation efficiency of Phe- and Asp-enriched mRNAs. Inactivation of trmB led to decreased KatA and KatB protein abundance and decreased catalase activity, resulting in H(2)O(2)-sensitive phenotype. Taken together, our observations reveal a novel role of m(7)G46 tRNA modification in oxidative stress response through translational regulation of Phe- and Asp-enriched genes, such as katA and katB. |
format | Online Article Text |
id | pubmed-6755087 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-67550872019-09-26 TrmB, a tRNA m(7)G46 methyltransferase, plays a role in hydrogen peroxide resistance and positively modulates the translation of katA and katB mRNAs in Pseudomonas aeruginosa Thongdee, Narumon Jaroensuk, Juthamas Atichartpongkul, Sopapan Chittrakanwong, Jurairat Chooyoung, Kamonchanok Srimahaeak, Thanyaporn Chaiyen, Pimchai Vattanaviboon, Paiboon Mongkolsuk, Skorn Fuangthong, Mayuree Nucleic Acids Res Nucleic Acid Enzymes Cellular response to oxidative stress is a crucial mechanism that promotes the survival of Pseudomonas aeruginosa during infection. However, the translational regulation of oxidative stress response remains largely unknown. Here, we reveal a tRNA modification-mediated translational response to H(2)O(2) in P. aeruginosa. We demonstrated that the P. aeruginosa trmB gene encodes a tRNA guanine (46)-N7-methyltransferase that catalyzes the formation of m(7)G46 in the tRNA variable loop. Twenty-three tRNA substrates of TrmB with a guanosine residue at position 46 were identified, including 11 novel tRNA substrates. We showed that loss of trmB had a strong negative effect on the translation of Phe- and Asp-enriched mRNAs. The trmB-mediated m(7)G modification modulated the expression of the catalase genes katA and katB, which are enriched with Phe/Asp codons at the translational level. In response to H(2)O(2) exposure, the level of m(7)G modification increased, consistent with the increased translation efficiency of Phe- and Asp-enriched mRNAs. Inactivation of trmB led to decreased KatA and KatB protein abundance and decreased catalase activity, resulting in H(2)O(2)-sensitive phenotype. Taken together, our observations reveal a novel role of m(7)G46 tRNA modification in oxidative stress response through translational regulation of Phe- and Asp-enriched genes, such as katA and katB. Oxford University Press 2019-09-26 2019-08-20 /pmc/articles/PMC6755087/ /pubmed/31428787 http://dx.doi.org/10.1093/nar/gkz702 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Nucleic Acid Enzymes Thongdee, Narumon Jaroensuk, Juthamas Atichartpongkul, Sopapan Chittrakanwong, Jurairat Chooyoung, Kamonchanok Srimahaeak, Thanyaporn Chaiyen, Pimchai Vattanaviboon, Paiboon Mongkolsuk, Skorn Fuangthong, Mayuree TrmB, a tRNA m(7)G46 methyltransferase, plays a role in hydrogen peroxide resistance and positively modulates the translation of katA and katB mRNAs in Pseudomonas aeruginosa |
title | TrmB, a tRNA m(7)G46 methyltransferase, plays a role in hydrogen peroxide resistance and positively modulates the translation of katA and katB mRNAs in Pseudomonas aeruginosa |
title_full | TrmB, a tRNA m(7)G46 methyltransferase, plays a role in hydrogen peroxide resistance and positively modulates the translation of katA and katB mRNAs in Pseudomonas aeruginosa |
title_fullStr | TrmB, a tRNA m(7)G46 methyltransferase, plays a role in hydrogen peroxide resistance and positively modulates the translation of katA and katB mRNAs in Pseudomonas aeruginosa |
title_full_unstemmed | TrmB, a tRNA m(7)G46 methyltransferase, plays a role in hydrogen peroxide resistance and positively modulates the translation of katA and katB mRNAs in Pseudomonas aeruginosa |
title_short | TrmB, a tRNA m(7)G46 methyltransferase, plays a role in hydrogen peroxide resistance and positively modulates the translation of katA and katB mRNAs in Pseudomonas aeruginosa |
title_sort | trmb, a trna m(7)g46 methyltransferase, plays a role in hydrogen peroxide resistance and positively modulates the translation of kata and katb mrnas in pseudomonas aeruginosa |
topic | Nucleic Acid Enzymes |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6755087/ https://www.ncbi.nlm.nih.gov/pubmed/31428787 http://dx.doi.org/10.1093/nar/gkz702 |
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