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Silencing of MALAT1 inhibits migration and invasion by sponging miR-1-3p in prostate cancer cells

Prostate cancer is a common malignancy with a high mortality rate. Long non-coding RNA metastasis associated with lung adenocarcinoma transcript 1 (MALAT1) has been reported to serve tumor-promoting roles. However, the underlying mechanism requires further examination. In the present study, it was d...

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Autores principales: Dai, Xiaofan, Liang, Zuowen, Liu, Lingyun, Guo, Kaimin, Xu, Shengqi, Wang, Hongliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6755148/
https://www.ncbi.nlm.nih.gov/pubmed/31485645
http://dx.doi.org/10.3892/mmr.2019.10602
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author Dai, Xiaofan
Liang, Zuowen
Liu, Lingyun
Guo, Kaimin
Xu, Shengqi
Wang, Hongliang
author_facet Dai, Xiaofan
Liang, Zuowen
Liu, Lingyun
Guo, Kaimin
Xu, Shengqi
Wang, Hongliang
author_sort Dai, Xiaofan
collection PubMed
description Prostate cancer is a common malignancy with a high mortality rate. Long non-coding RNA metastasis associated with lung adenocarcinoma transcript 1 (MALAT1) has been reported to serve tumor-promoting roles. However, the underlying mechanism requires further examination. In the present study, it was demonstrated that MALAT1 was increased while microRNA (miR/miRNA)-1-3p was decreased in prostate cancer cell lines. The silencing of MALAT1 inhibited migration, invasion and epithelial-mesenchymal transition, when epithelial (E)-cadherin expression level was increased, and neural (N)-cadherin, vimentin, Slug and Snail expression levels were decreased. Dual-luciferase reporter assay results demonstrated that miR-1-3p bound to MALAT1 and coronin 1C (CORO1C) 3′ untranslated region, and MALAT1 competed with CORO1C for the binding sites of miR-1-3p. MALAT1 inhibited the expression of miR-1-3p and vice versa. MALAT1 knockdown induced the decline of CORO1C, which was subsequently recovered by the miR-1-3p inhibitor. In addition, by inhibiting miR-1-3p or overexpressing CORO1C, the silencing of MALAT1-induced phenotypic alterations were restored. In conclusion, MALAT1 serving as a degradable miRNA sponge, may sequester miR-1-3p from CORO1C and by silencing MALAT1, migration, invasion and epithelial-mesenchymal transition may be inhibited in prostate cancer cells. MALAT1 and CORO1C may serve as novel clinical therapeutic targets for prostate cancer.
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spelling pubmed-67551482019-09-25 Silencing of MALAT1 inhibits migration and invasion by sponging miR-1-3p in prostate cancer cells Dai, Xiaofan Liang, Zuowen Liu, Lingyun Guo, Kaimin Xu, Shengqi Wang, Hongliang Mol Med Rep Articles Prostate cancer is a common malignancy with a high mortality rate. Long non-coding RNA metastasis associated with lung adenocarcinoma transcript 1 (MALAT1) has been reported to serve tumor-promoting roles. However, the underlying mechanism requires further examination. In the present study, it was demonstrated that MALAT1 was increased while microRNA (miR/miRNA)-1-3p was decreased in prostate cancer cell lines. The silencing of MALAT1 inhibited migration, invasion and epithelial-mesenchymal transition, when epithelial (E)-cadherin expression level was increased, and neural (N)-cadherin, vimentin, Slug and Snail expression levels were decreased. Dual-luciferase reporter assay results demonstrated that miR-1-3p bound to MALAT1 and coronin 1C (CORO1C) 3′ untranslated region, and MALAT1 competed with CORO1C for the binding sites of miR-1-3p. MALAT1 inhibited the expression of miR-1-3p and vice versa. MALAT1 knockdown induced the decline of CORO1C, which was subsequently recovered by the miR-1-3p inhibitor. In addition, by inhibiting miR-1-3p or overexpressing CORO1C, the silencing of MALAT1-induced phenotypic alterations were restored. In conclusion, MALAT1 serving as a degradable miRNA sponge, may sequester miR-1-3p from CORO1C and by silencing MALAT1, migration, invasion and epithelial-mesenchymal transition may be inhibited in prostate cancer cells. MALAT1 and CORO1C may serve as novel clinical therapeutic targets for prostate cancer. D.A. Spandidos 2019-10 2019-08-22 /pmc/articles/PMC6755148/ /pubmed/31485645 http://dx.doi.org/10.3892/mmr.2019.10602 Text en Copyright: © Dai et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Dai, Xiaofan
Liang, Zuowen
Liu, Lingyun
Guo, Kaimin
Xu, Shengqi
Wang, Hongliang
Silencing of MALAT1 inhibits migration and invasion by sponging miR-1-3p in prostate cancer cells
title Silencing of MALAT1 inhibits migration and invasion by sponging miR-1-3p in prostate cancer cells
title_full Silencing of MALAT1 inhibits migration and invasion by sponging miR-1-3p in prostate cancer cells
title_fullStr Silencing of MALAT1 inhibits migration and invasion by sponging miR-1-3p in prostate cancer cells
title_full_unstemmed Silencing of MALAT1 inhibits migration and invasion by sponging miR-1-3p in prostate cancer cells
title_short Silencing of MALAT1 inhibits migration and invasion by sponging miR-1-3p in prostate cancer cells
title_sort silencing of malat1 inhibits migration and invasion by sponging mir-1-3p in prostate cancer cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6755148/
https://www.ncbi.nlm.nih.gov/pubmed/31485645
http://dx.doi.org/10.3892/mmr.2019.10602
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