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Expression and function of Toll-like receptors in peripheral blood mononuclear cells in patients with ankylosing spondylitis

Ankylosing spondylitis (AS) is a common chronic inflammatory autoimmune disease. Toll-like receptors (TLRs) are involved in non-specific immunity. In the present study, the roles of TLRs in AS were investigated. The levels of inflammatory cytokines were detected by ELISA and reverse transcription-qu...

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Autores principales: Zhang, Jun, Xu, Rongming, Wu, Lei, Jiang, Jihong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6755152/
https://www.ncbi.nlm.nih.gov/pubmed/31485664
http://dx.doi.org/10.3892/mmr.2019.10631
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author Zhang, Jun
Xu, Rongming
Wu, Lei
Jiang, Jihong
author_facet Zhang, Jun
Xu, Rongming
Wu, Lei
Jiang, Jihong
author_sort Zhang, Jun
collection PubMed
description Ankylosing spondylitis (AS) is a common chronic inflammatory autoimmune disease. Toll-like receptors (TLRs) are involved in non-specific immunity. In the present study, the roles of TLRs in AS were investigated. The levels of inflammatory cytokines were detected by ELISA and reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The expression levels of TLRs and nuclear factor-κB (NF-κB) signaling-associated factors were determined via RT-qPCR and western blot analyses. It was observed that the levels of interleukin (IL)-6, tumor necrosis factor-α (TNF-α), C-reactive protein, TLR4 and TLR5 were increased in patients with AS, whereas those of IL-10 and TLR3 were decreased. Pomalidomide, a TNF-α release inhibitor, reduced the expression of IL-6, TNF-α, TLR4, TLR5 and phosphorylated-p65, and upregulated that of IL-10, TLR3 and p65 in peripheral blood mononuclear cells from patients with AS. Treatment of patients with infliximab, an anti-TNF-α monoclonal antibody, induced similar effects in vivo. In conclusion, it was revealed that inhibition of TNF-α suppressed inflammatory responses in patients with AS, increased the expression of TLR3 and decreased NF-κB signaling, and the expression of TLR4 and TLR5. The results indicated that TLRs and the NF-κB signaling pathway were involved in the regulation of inflammatory responses in AS. These findings provided insight into the mechanisms underlying the development of AS and potential novel therapeutic approaches.
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spelling pubmed-67551522019-09-25 Expression and function of Toll-like receptors in peripheral blood mononuclear cells in patients with ankylosing spondylitis Zhang, Jun Xu, Rongming Wu, Lei Jiang, Jihong Mol Med Rep Articles Ankylosing spondylitis (AS) is a common chronic inflammatory autoimmune disease. Toll-like receptors (TLRs) are involved in non-specific immunity. In the present study, the roles of TLRs in AS were investigated. The levels of inflammatory cytokines were detected by ELISA and reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The expression levels of TLRs and nuclear factor-κB (NF-κB) signaling-associated factors were determined via RT-qPCR and western blot analyses. It was observed that the levels of interleukin (IL)-6, tumor necrosis factor-α (TNF-α), C-reactive protein, TLR4 and TLR5 were increased in patients with AS, whereas those of IL-10 and TLR3 were decreased. Pomalidomide, a TNF-α release inhibitor, reduced the expression of IL-6, TNF-α, TLR4, TLR5 and phosphorylated-p65, and upregulated that of IL-10, TLR3 and p65 in peripheral blood mononuclear cells from patients with AS. Treatment of patients with infliximab, an anti-TNF-α monoclonal antibody, induced similar effects in vivo. In conclusion, it was revealed that inhibition of TNF-α suppressed inflammatory responses in patients with AS, increased the expression of TLR3 and decreased NF-κB signaling, and the expression of TLR4 and TLR5. The results indicated that TLRs and the NF-κB signaling pathway were involved in the regulation of inflammatory responses in AS. These findings provided insight into the mechanisms underlying the development of AS and potential novel therapeutic approaches. D.A. Spandidos 2019-10 2019-09-02 /pmc/articles/PMC6755152/ /pubmed/31485664 http://dx.doi.org/10.3892/mmr.2019.10631 Text en Copyright: © Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Zhang, Jun
Xu, Rongming
Wu, Lei
Jiang, Jihong
Expression and function of Toll-like receptors in peripheral blood mononuclear cells in patients with ankylosing spondylitis
title Expression and function of Toll-like receptors in peripheral blood mononuclear cells in patients with ankylosing spondylitis
title_full Expression and function of Toll-like receptors in peripheral blood mononuclear cells in patients with ankylosing spondylitis
title_fullStr Expression and function of Toll-like receptors in peripheral blood mononuclear cells in patients with ankylosing spondylitis
title_full_unstemmed Expression and function of Toll-like receptors in peripheral blood mononuclear cells in patients with ankylosing spondylitis
title_short Expression and function of Toll-like receptors in peripheral blood mononuclear cells in patients with ankylosing spondylitis
title_sort expression and function of toll-like receptors in peripheral blood mononuclear cells in patients with ankylosing spondylitis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6755152/
https://www.ncbi.nlm.nih.gov/pubmed/31485664
http://dx.doi.org/10.3892/mmr.2019.10631
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