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MicroRNA-155 inhibits the proliferation of CD8(+) T cells via upregulating regulatory T cells in vitiligo

It has been reported that loss and degradation of epidermal melanocytes is closely associated with the pathogenesis of vitiligo. In addition, CD8(+) T and regulatory T (Treg) cells serve an important role during these two processes. MicroRNA-155 (miR-155) is known to contribute to the pathogenesis o...

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Autores principales: Lv, Mingfen, Li, Zhengjun, Liu, Jingjing, Lin, Fan, Zhang, Qianwen, Li, Zhiming, Wang, Yi, Wang, Keyu, Xu, Yunsheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6755204/
https://www.ncbi.nlm.nih.gov/pubmed/31485649
http://dx.doi.org/10.3892/mmr.2019.10607
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author Lv, Mingfen
Li, Zhengjun
Liu, Jingjing
Lin, Fan
Zhang, Qianwen
Li, Zhiming
Wang, Yi
Wang, Keyu
Xu, Yunsheng
author_facet Lv, Mingfen
Li, Zhengjun
Liu, Jingjing
Lin, Fan
Zhang, Qianwen
Li, Zhiming
Wang, Yi
Wang, Keyu
Xu, Yunsheng
author_sort Lv, Mingfen
collection PubMed
description It has been reported that loss and degradation of epidermal melanocytes is closely associated with the pathogenesis of vitiligo. In addition, CD8(+) T and regulatory T (Treg) cells serve an important role during these two processes. MicroRNA-155 (miR-155) is known to contribute to the pathogenesis of vitiligo; however, the mechanism by which miR-155 regulates the development of vitiligo remains unclear. In the present study, naïve T and CD8(+) T cells were isolated from a patient with non-segmental vitiligo by flow cytometry. The cells were differentiated into Treg cells by treatment with interleukin-2, transforming growth factor-β and retinoic acid. In addition, miR-155 agonists and antagonists were used to investigate the effect of miR-155 on the proliferation of CD8(+) T cells, Treg cells and melanocytes. The results demonstrated that the miR-155 agonist significantly decreased the rate of CD8(+) T cell growth, as well as promoted the proliferation of melanocytes by inducing an increase in the percentage of Treg cells. By contrast, the miR-155 antagonist inhibited the proliferation of melanocytes by decreasing the percentage of Treg cells. miR-155 protected melanocyte survival by increasing the number of Treg cells and by decreasing the number of CD8(+) T cells. Therefore, these data may provide a new prospect for the treatment of vitiligo.
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spelling pubmed-67552042019-09-25 MicroRNA-155 inhibits the proliferation of CD8(+) T cells via upregulating regulatory T cells in vitiligo Lv, Mingfen Li, Zhengjun Liu, Jingjing Lin, Fan Zhang, Qianwen Li, Zhiming Wang, Yi Wang, Keyu Xu, Yunsheng Mol Med Rep Articles It has been reported that loss and degradation of epidermal melanocytes is closely associated with the pathogenesis of vitiligo. In addition, CD8(+) T and regulatory T (Treg) cells serve an important role during these two processes. MicroRNA-155 (miR-155) is known to contribute to the pathogenesis of vitiligo; however, the mechanism by which miR-155 regulates the development of vitiligo remains unclear. In the present study, naïve T and CD8(+) T cells were isolated from a patient with non-segmental vitiligo by flow cytometry. The cells were differentiated into Treg cells by treatment with interleukin-2, transforming growth factor-β and retinoic acid. In addition, miR-155 agonists and antagonists were used to investigate the effect of miR-155 on the proliferation of CD8(+) T cells, Treg cells and melanocytes. The results demonstrated that the miR-155 agonist significantly decreased the rate of CD8(+) T cell growth, as well as promoted the proliferation of melanocytes by inducing an increase in the percentage of Treg cells. By contrast, the miR-155 antagonist inhibited the proliferation of melanocytes by decreasing the percentage of Treg cells. miR-155 protected melanocyte survival by increasing the number of Treg cells and by decreasing the number of CD8(+) T cells. Therefore, these data may provide a new prospect for the treatment of vitiligo. D.A. Spandidos 2019-10 2019-08-23 /pmc/articles/PMC6755204/ /pubmed/31485649 http://dx.doi.org/10.3892/mmr.2019.10607 Text en Copyright: © Lv et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Lv, Mingfen
Li, Zhengjun
Liu, Jingjing
Lin, Fan
Zhang, Qianwen
Li, Zhiming
Wang, Yi
Wang, Keyu
Xu, Yunsheng
MicroRNA-155 inhibits the proliferation of CD8(+) T cells via upregulating regulatory T cells in vitiligo
title MicroRNA-155 inhibits the proliferation of CD8(+) T cells via upregulating regulatory T cells in vitiligo
title_full MicroRNA-155 inhibits the proliferation of CD8(+) T cells via upregulating regulatory T cells in vitiligo
title_fullStr MicroRNA-155 inhibits the proliferation of CD8(+) T cells via upregulating regulatory T cells in vitiligo
title_full_unstemmed MicroRNA-155 inhibits the proliferation of CD8(+) T cells via upregulating regulatory T cells in vitiligo
title_short MicroRNA-155 inhibits the proliferation of CD8(+) T cells via upregulating regulatory T cells in vitiligo
title_sort microrna-155 inhibits the proliferation of cd8(+) t cells via upregulating regulatory t cells in vitiligo
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6755204/
https://www.ncbi.nlm.nih.gov/pubmed/31485649
http://dx.doi.org/10.3892/mmr.2019.10607
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