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Hepatic NPC1L1 overexpression attenuates alcoholic autophagy in mice

Alcohol consumption causes liver steatosis in humans. Metabolic disorders of lipids are one of the factors that cause liver steatosis in hepatocytes. Hepatic Niemann-Pick C1-like 1 (NPC1L1) regulates lipid homeostasis in mammals. The relationship between NPC1L1 and autophagy in those with a history...

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Autores principales: Wang, Youlin, Yang, Pan, Zhang, Bo, Ding, Yao, Lei, Shun, Hou, Yuning, Guan, Xiaoqing, Li, Qingwang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6755247/
https://www.ncbi.nlm.nih.gov/pubmed/31432115
http://dx.doi.org/10.3892/mmr.2019.10549
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author Wang, Youlin
Yang, Pan
Zhang, Bo
Ding, Yao
Lei, Shun
Hou, Yuning
Guan, Xiaoqing
Li, Qingwang
author_facet Wang, Youlin
Yang, Pan
Zhang, Bo
Ding, Yao
Lei, Shun
Hou, Yuning
Guan, Xiaoqing
Li, Qingwang
author_sort Wang, Youlin
collection PubMed
description Alcohol consumption causes liver steatosis in humans. Metabolic disorders of lipids are one of the factors that cause liver steatosis in hepatocytes. Hepatic Niemann-Pick C1-like 1 (NPC1L1) regulates lipid homeostasis in mammals. The relationship between NPC1L1 and autophagy in those with a history of alcohol abuse is unclear. The present study aimed to investigate the function of NPC1L1 in the activation of hepatic autophagy in a mouse model with a human (h)NPC1L1 transgene under alcohol feeding conditions. The mice expressing hNPC1L1 (Ad-L1) or controls (Ad-null) were created by retro-orbital adenovirus injection. The Ad-L1 and Ad-null mice were fed with alcohol or a non-alcoholic diet to mimic chronic alcohol consumption in humans. Hepatic autophagy was demonstrated in isolated primary hepatocytes by monitoring autophagic vacuoles under fluorescence microscopy, and by western blotting for autophagic makers. Isolated hepatocytes from the livers of Ad-L1 mice were treated with different doses of ezetimibe to study the restoration of autophagy. Chronic alcohol feeding caused liver injury and steatosis, shown by significantly higher levels of plasma alanine transaminase and aspartate transaminase activity, and by hematoxylin and eosin staining in Ad-L1 and Ad-null mice. Compared to Ad-null control mice, the microtubule-associated proteins 1A/1B light chain 3 (LC3) particles in the isolated hepatocytes of Ad-L1 mice were decreased, both under alcohol and non-alcoholic feeding. The ratio of LC3II/LC3I was significantly decreased, and the level of p62/sequestosome-1 protein was significantly increased in Ad-L1 mice compared with Ad-null mice after alcohol feeding. Levels of LC3II protein were statistically increased in hepatocytes isolated from Ad-L1 mice with ezetimibe treatment. The increase in LC3II expression was dose dependent. Within the tested range, it reached its highest level at 40 µM. The livers of Ad-L1 mice represent a more human-like state for the study of hepatic autophagy. Hepatic expression of human NPC1L1 resulted in an inhibition of autophagy; it may contribute to alcoholic fatty liver disease in humans.
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spelling pubmed-67552472019-09-25 Hepatic NPC1L1 overexpression attenuates alcoholic autophagy in mice Wang, Youlin Yang, Pan Zhang, Bo Ding, Yao Lei, Shun Hou, Yuning Guan, Xiaoqing Li, Qingwang Mol Med Rep Articles Alcohol consumption causes liver steatosis in humans. Metabolic disorders of lipids are one of the factors that cause liver steatosis in hepatocytes. Hepatic Niemann-Pick C1-like 1 (NPC1L1) regulates lipid homeostasis in mammals. The relationship between NPC1L1 and autophagy in those with a history of alcohol abuse is unclear. The present study aimed to investigate the function of NPC1L1 in the activation of hepatic autophagy in a mouse model with a human (h)NPC1L1 transgene under alcohol feeding conditions. The mice expressing hNPC1L1 (Ad-L1) or controls (Ad-null) were created by retro-orbital adenovirus injection. The Ad-L1 and Ad-null mice were fed with alcohol or a non-alcoholic diet to mimic chronic alcohol consumption in humans. Hepatic autophagy was demonstrated in isolated primary hepatocytes by monitoring autophagic vacuoles under fluorescence microscopy, and by western blotting for autophagic makers. Isolated hepatocytes from the livers of Ad-L1 mice were treated with different doses of ezetimibe to study the restoration of autophagy. Chronic alcohol feeding caused liver injury and steatosis, shown by significantly higher levels of plasma alanine transaminase and aspartate transaminase activity, and by hematoxylin and eosin staining in Ad-L1 and Ad-null mice. Compared to Ad-null control mice, the microtubule-associated proteins 1A/1B light chain 3 (LC3) particles in the isolated hepatocytes of Ad-L1 mice were decreased, both under alcohol and non-alcoholic feeding. The ratio of LC3II/LC3I was significantly decreased, and the level of p62/sequestosome-1 protein was significantly increased in Ad-L1 mice compared with Ad-null mice after alcohol feeding. Levels of LC3II protein were statistically increased in hepatocytes isolated from Ad-L1 mice with ezetimibe treatment. The increase in LC3II expression was dose dependent. Within the tested range, it reached its highest level at 40 µM. The livers of Ad-L1 mice represent a more human-like state for the study of hepatic autophagy. Hepatic expression of human NPC1L1 resulted in an inhibition of autophagy; it may contribute to alcoholic fatty liver disease in humans. D.A. Spandidos 2019-10 2019-08-01 /pmc/articles/PMC6755247/ /pubmed/31432115 http://dx.doi.org/10.3892/mmr.2019.10549 Text en Copyright: © Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Wang, Youlin
Yang, Pan
Zhang, Bo
Ding, Yao
Lei, Shun
Hou, Yuning
Guan, Xiaoqing
Li, Qingwang
Hepatic NPC1L1 overexpression attenuates alcoholic autophagy in mice
title Hepatic NPC1L1 overexpression attenuates alcoholic autophagy in mice
title_full Hepatic NPC1L1 overexpression attenuates alcoholic autophagy in mice
title_fullStr Hepatic NPC1L1 overexpression attenuates alcoholic autophagy in mice
title_full_unstemmed Hepatic NPC1L1 overexpression attenuates alcoholic autophagy in mice
title_short Hepatic NPC1L1 overexpression attenuates alcoholic autophagy in mice
title_sort hepatic npc1l1 overexpression attenuates alcoholic autophagy in mice
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6755247/
https://www.ncbi.nlm.nih.gov/pubmed/31432115
http://dx.doi.org/10.3892/mmr.2019.10549
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