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MiR-599 serves a suppressive role in anaplastic thyroid cancer by activating the T-cell intracellular antigen

Anaplastic thyroid cancer (ATC) has a mean survival time of 6 months and accounts for 1–2% of all thyroid tumors. Understanding the underlying molecular mechanisms of carcinogenesis and progression in ATC would contribute to the identification of novel therapeutic targets. A previous study revealed...

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Autores principales: Bi, Jian Wei, Zou, Yan Liang, Qian, Jian Tong, Chen, Wen Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6755273/
https://www.ncbi.nlm.nih.gov/pubmed/31555352
http://dx.doi.org/10.3892/etm.2019.7864
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author Bi, Jian Wei
Zou, Yan Liang
Qian, Jian Tong
Chen, Wen Bin
author_facet Bi, Jian Wei
Zou, Yan Liang
Qian, Jian Tong
Chen, Wen Bin
author_sort Bi, Jian Wei
collection PubMed
description Anaplastic thyroid cancer (ATC) has a mean survival time of 6 months and accounts for 1–2% of all thyroid tumors. Understanding the underlying molecular mechanisms of carcinogenesis and progression in ATC would contribute to the identification of novel therapeutic targets. A previous study revealed that microRNA (miR)-599 was associated with tumor initiation and development in certain types of cancer. However, the specific functions and mechanisms of miR-599 in ATC are poorly understood. The objective of the present study was to identify its expression, function and molecular mechanism in ATC. The expression levels of miR-599 in 10 pairs of surgical specimens and human ATC cell lines were examined by reverse transcription-quantitative polymerase chain reaction. Function assays illustrated that miR-599 overexpression not only suppressed KAT-18 cell viability, proliferation and metastasis in vitro and decreased tumor growth in the tumor xenograft model but also induced cell apoptosis. Furthermore, T-cell intracellular antigen (TIA1), a tumor suppressor, was confirmed as a direct target of miR-599. It was demonstrated that TIA1 silencing rescued the inhibitory effect of migration and invasion induced by the overexpression of miR-599 in KAT-18 cells. In conclusion, the present study revealed that miR-599 inhibited ATC cell growth and metastasis via activation of TIA1. Therefore miR-599 may be a novel molecular therapeutic target for ATC.
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spelling pubmed-67552732019-09-25 MiR-599 serves a suppressive role in anaplastic thyroid cancer by activating the T-cell intracellular antigen Bi, Jian Wei Zou, Yan Liang Qian, Jian Tong Chen, Wen Bin Exp Ther Med Articles Anaplastic thyroid cancer (ATC) has a mean survival time of 6 months and accounts for 1–2% of all thyroid tumors. Understanding the underlying molecular mechanisms of carcinogenesis and progression in ATC would contribute to the identification of novel therapeutic targets. A previous study revealed that microRNA (miR)-599 was associated with tumor initiation and development in certain types of cancer. However, the specific functions and mechanisms of miR-599 in ATC are poorly understood. The objective of the present study was to identify its expression, function and molecular mechanism in ATC. The expression levels of miR-599 in 10 pairs of surgical specimens and human ATC cell lines were examined by reverse transcription-quantitative polymerase chain reaction. Function assays illustrated that miR-599 overexpression not only suppressed KAT-18 cell viability, proliferation and metastasis in vitro and decreased tumor growth in the tumor xenograft model but also induced cell apoptosis. Furthermore, T-cell intracellular antigen (TIA1), a tumor suppressor, was confirmed as a direct target of miR-599. It was demonstrated that TIA1 silencing rescued the inhibitory effect of migration and invasion induced by the overexpression of miR-599 in KAT-18 cells. In conclusion, the present study revealed that miR-599 inhibited ATC cell growth and metastasis via activation of TIA1. Therefore miR-599 may be a novel molecular therapeutic target for ATC. D.A. Spandidos 2019-10 2019-08-07 /pmc/articles/PMC6755273/ /pubmed/31555352 http://dx.doi.org/10.3892/etm.2019.7864 Text en Copyright: © Bi et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Bi, Jian Wei
Zou, Yan Liang
Qian, Jian Tong
Chen, Wen Bin
MiR-599 serves a suppressive role in anaplastic thyroid cancer by activating the T-cell intracellular antigen
title MiR-599 serves a suppressive role in anaplastic thyroid cancer by activating the T-cell intracellular antigen
title_full MiR-599 serves a suppressive role in anaplastic thyroid cancer by activating the T-cell intracellular antigen
title_fullStr MiR-599 serves a suppressive role in anaplastic thyroid cancer by activating the T-cell intracellular antigen
title_full_unstemmed MiR-599 serves a suppressive role in anaplastic thyroid cancer by activating the T-cell intracellular antigen
title_short MiR-599 serves a suppressive role in anaplastic thyroid cancer by activating the T-cell intracellular antigen
title_sort mir-599 serves a suppressive role in anaplastic thyroid cancer by activating the t-cell intracellular antigen
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6755273/
https://www.ncbi.nlm.nih.gov/pubmed/31555352
http://dx.doi.org/10.3892/etm.2019.7864
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