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Quercetin has a protective effect on atherosclerosis via enhancement of autophagy in ApoE(−/−) mice
The present study examined the involvement of autophagy as a mechanism in the protective effect of quercetin (QUE) on atherosclerosis (AS) in ApoE(−/−) mice. An AS model was established by feeding ApoE(−/−) mice a high-fat diet (HFD). Mice were divided into four experimental groups: The model, QUE,...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6755277/ https://www.ncbi.nlm.nih.gov/pubmed/31555357 http://dx.doi.org/10.3892/etm.2019.7851 |
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author | Cao, Hui Jia, Qingling Shen, Dingzhu Yan, Li Chen, Chuan Xing, Sanli |
author_facet | Cao, Hui Jia, Qingling Shen, Dingzhu Yan, Li Chen, Chuan Xing, Sanli |
author_sort | Cao, Hui |
collection | PubMed |
description | The present study examined the involvement of autophagy as a mechanism in the protective effect of quercetin (QUE) on atherosclerosis (AS) in ApoE(−/−) mice. An AS model was established by feeding ApoE(−/−) mice a high-fat diet (HFD). Mice were divided into four experimental groups: The model, QUE, 3-methyladenine (3-MA) and QUE + 3-MA groups. Additionally, age-matched wild-type C57BL/6 mice were used as a Control group. Autophagosomes in the aorta were examined using a transmission electron microscope. Aorta pathology, serum lipid accumulation and collagen deposition were determined by hematoxylin and eosin, Oil Red O and Masson staining, respectively. The levels of cytokines, including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-18 (IL-18) were measured using ELISA assays. Protein levels of mTOR, microtubule associated protein 1 light chain 3a (LC3), P53 and cyclin dependent kinase inhibitor 1A (P21) in the aorta were analyzed using western blotting. ApoE(−/−) mice which were fed HFD exhibited substantial AS pathology, no autophagosomes, higher levels of TNF-α, IL-1β, IL-18 and mTOR and lower ratios of LC3 II/I. All these alterations were ameliorated and aggravated by QUE and 3-MA treatment, respectively. The inhibition of AS by QUE may be associated with the enhancement of autophagy and upregulation of P21 and P53 expression. |
format | Online Article Text |
id | pubmed-6755277 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-67552772019-09-25 Quercetin has a protective effect on atherosclerosis via enhancement of autophagy in ApoE(−/−) mice Cao, Hui Jia, Qingling Shen, Dingzhu Yan, Li Chen, Chuan Xing, Sanli Exp Ther Med Articles The present study examined the involvement of autophagy as a mechanism in the protective effect of quercetin (QUE) on atherosclerosis (AS) in ApoE(−/−) mice. An AS model was established by feeding ApoE(−/−) mice a high-fat diet (HFD). Mice were divided into four experimental groups: The model, QUE, 3-methyladenine (3-MA) and QUE + 3-MA groups. Additionally, age-matched wild-type C57BL/6 mice were used as a Control group. Autophagosomes in the aorta were examined using a transmission electron microscope. Aorta pathology, serum lipid accumulation and collagen deposition were determined by hematoxylin and eosin, Oil Red O and Masson staining, respectively. The levels of cytokines, including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-18 (IL-18) were measured using ELISA assays. Protein levels of mTOR, microtubule associated protein 1 light chain 3a (LC3), P53 and cyclin dependent kinase inhibitor 1A (P21) in the aorta were analyzed using western blotting. ApoE(−/−) mice which were fed HFD exhibited substantial AS pathology, no autophagosomes, higher levels of TNF-α, IL-1β, IL-18 and mTOR and lower ratios of LC3 II/I. All these alterations were ameliorated and aggravated by QUE and 3-MA treatment, respectively. The inhibition of AS by QUE may be associated with the enhancement of autophagy and upregulation of P21 and P53 expression. D.A. Spandidos 2019-10 2019-08-05 /pmc/articles/PMC6755277/ /pubmed/31555357 http://dx.doi.org/10.3892/etm.2019.7851 Text en Copyright: © Cao et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Cao, Hui Jia, Qingling Shen, Dingzhu Yan, Li Chen, Chuan Xing, Sanli Quercetin has a protective effect on atherosclerosis via enhancement of autophagy in ApoE(−/−) mice |
title | Quercetin has a protective effect on atherosclerosis via enhancement of autophagy in ApoE(−/−) mice |
title_full | Quercetin has a protective effect on atherosclerosis via enhancement of autophagy in ApoE(−/−) mice |
title_fullStr | Quercetin has a protective effect on atherosclerosis via enhancement of autophagy in ApoE(−/−) mice |
title_full_unstemmed | Quercetin has a protective effect on atherosclerosis via enhancement of autophagy in ApoE(−/−) mice |
title_short | Quercetin has a protective effect on atherosclerosis via enhancement of autophagy in ApoE(−/−) mice |
title_sort | quercetin has a protective effect on atherosclerosis via enhancement of autophagy in apoe(−/−) mice |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6755277/ https://www.ncbi.nlm.nih.gov/pubmed/31555357 http://dx.doi.org/10.3892/etm.2019.7851 |
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