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Investigation of leukocyte RHO/ROCK gene expressions in patients with non-valvular atrial fibrillation
Atrial fibrillation (AF) is an arrhythmia caused by disorganized electrical activity in the atria, and it is an important cause of mortality and morbidity. There is a limited data about Rho/Rho-kinase (ROCK) pathway contribute to AF development. The aim of the present study was to elucidate leukocyt...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6755446/ https://www.ncbi.nlm.nih.gov/pubmed/31572525 http://dx.doi.org/10.3892/etm.2019.7929 |
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author | Düzen, Irfan V. Yavuz, Fethi Vuruskan, Ertan Saracoglu, Erhan Poyraz, Fatih Cekici, Yusuf Alıcı, Hayri Göksülük, Hüseyin Candemir, Basar Sucu, Murat Demiryürek, Abdullah T. |
author_facet | Düzen, Irfan V. Yavuz, Fethi Vuruskan, Ertan Saracoglu, Erhan Poyraz, Fatih Cekici, Yusuf Alıcı, Hayri Göksülük, Hüseyin Candemir, Basar Sucu, Murat Demiryürek, Abdullah T. |
author_sort | Düzen, Irfan V. |
collection | PubMed |
description | Atrial fibrillation (AF) is an arrhythmia caused by disorganized electrical activity in the atria, and it is an important cause of mortality and morbidity. There is a limited data about Rho/Rho-kinase (ROCK) pathway contribute to AF development. The aim of the present study was to elucidate leukocyte RHO/ROCK gene expressions in patients with non-valvular AF (NVAF). A total of 37 NVAF patients and 47 age and sex-matched controls were included in this study. mRNA was extracted from leukocytes, and real-time polymerase chain reaction was used for gene expression analysis. A marked increase in ROCK1 and ROCK2 gene expressions in patients with NVAF was observed (P<0.0001). The present study detected significant elevations in RHOBTB2, RND3 (RHOE), RHOC, RHOG, RHOH, RAC3, RHOB, RHOD, RHOV, RHOBTB1, RND2, RND1 and RHOJ gene expressions (P<0.01). However, there were marked decreases in CDC42, RAC2, and RHOQ gene expressions in patients with NVAF. No significant modifications were seen in the other Rho GTPase proteins RHOA, RAC1, RHOF, RHOU and RHOBTB3. To the best of our knowledge, the present study is the first to provide data that gene expression of leukocyte RHO/ROCK may contribute to the NVAF pathogenesis through activated leukocytes, which promotes the immune or inflammatory cascade. |
format | Online Article Text |
id | pubmed-6755446 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-67554462019-09-30 Investigation of leukocyte RHO/ROCK gene expressions in patients with non-valvular atrial fibrillation Düzen, Irfan V. Yavuz, Fethi Vuruskan, Ertan Saracoglu, Erhan Poyraz, Fatih Cekici, Yusuf Alıcı, Hayri Göksülük, Hüseyin Candemir, Basar Sucu, Murat Demiryürek, Abdullah T. Exp Ther Med Articles Atrial fibrillation (AF) is an arrhythmia caused by disorganized electrical activity in the atria, and it is an important cause of mortality and morbidity. There is a limited data about Rho/Rho-kinase (ROCK) pathway contribute to AF development. The aim of the present study was to elucidate leukocyte RHO/ROCK gene expressions in patients with non-valvular AF (NVAF). A total of 37 NVAF patients and 47 age and sex-matched controls were included in this study. mRNA was extracted from leukocytes, and real-time polymerase chain reaction was used for gene expression analysis. A marked increase in ROCK1 and ROCK2 gene expressions in patients with NVAF was observed (P<0.0001). The present study detected significant elevations in RHOBTB2, RND3 (RHOE), RHOC, RHOG, RHOH, RAC3, RHOB, RHOD, RHOV, RHOBTB1, RND2, RND1 and RHOJ gene expressions (P<0.01). However, there were marked decreases in CDC42, RAC2, and RHOQ gene expressions in patients with NVAF. No significant modifications were seen in the other Rho GTPase proteins RHOA, RAC1, RHOF, RHOU and RHOBTB3. To the best of our knowledge, the present study is the first to provide data that gene expression of leukocyte RHO/ROCK may contribute to the NVAF pathogenesis through activated leukocytes, which promotes the immune or inflammatory cascade. D.A. Spandidos 2019-10 2019-08-20 /pmc/articles/PMC6755446/ /pubmed/31572525 http://dx.doi.org/10.3892/etm.2019.7929 Text en Copyright: © Düzen et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Düzen, Irfan V. Yavuz, Fethi Vuruskan, Ertan Saracoglu, Erhan Poyraz, Fatih Cekici, Yusuf Alıcı, Hayri Göksülük, Hüseyin Candemir, Basar Sucu, Murat Demiryürek, Abdullah T. Investigation of leukocyte RHO/ROCK gene expressions in patients with non-valvular atrial fibrillation |
title | Investigation of leukocyte RHO/ROCK gene expressions in patients with non-valvular atrial fibrillation |
title_full | Investigation of leukocyte RHO/ROCK gene expressions in patients with non-valvular atrial fibrillation |
title_fullStr | Investigation of leukocyte RHO/ROCK gene expressions in patients with non-valvular atrial fibrillation |
title_full_unstemmed | Investigation of leukocyte RHO/ROCK gene expressions in patients with non-valvular atrial fibrillation |
title_short | Investigation of leukocyte RHO/ROCK gene expressions in patients with non-valvular atrial fibrillation |
title_sort | investigation of leukocyte rho/rock gene expressions in patients with non-valvular atrial fibrillation |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6755446/ https://www.ncbi.nlm.nih.gov/pubmed/31572525 http://dx.doi.org/10.3892/etm.2019.7929 |
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