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Perturbed Microbial Ecology in Myasthenia Gravis: Evidence from the Gut Microbiome and Fecal Metabolome

Myasthenia gravis (MG) is a devastating acquired autoimmune disease. Emerging evidence indicates that the gut microbiome plays a key role in maintaining immune system homeostasis. This work reports that MG is characterized by decreased α‐phylogenetic diversity, and significantly disturbed gut microb...

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Autores principales: Zheng, Peng, Li, Yifan, Wu, Jing, Zhang, Hanping, Huang, Yu, Tan, Xunmin, Pan, Junxi, Duan, Jiajia, Liang, Weiwei, Yin, Bangmin, Deng, Fengli, Perry, Seth W., Wong, Ma‐Li, Licinio, Julio, Wei, Hong, Yu, Gang, Xie, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6755540/
https://www.ncbi.nlm.nih.gov/pubmed/31559142
http://dx.doi.org/10.1002/advs.201901441
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author Zheng, Peng
Li, Yifan
Wu, Jing
Zhang, Hanping
Huang, Yu
Tan, Xunmin
Pan, Junxi
Duan, Jiajia
Liang, Weiwei
Yin, Bangmin
Deng, Fengli
Perry, Seth W.
Wong, Ma‐Li
Licinio, Julio
Wei, Hong
Yu, Gang
Xie, Peng
author_facet Zheng, Peng
Li, Yifan
Wu, Jing
Zhang, Hanping
Huang, Yu
Tan, Xunmin
Pan, Junxi
Duan, Jiajia
Liang, Weiwei
Yin, Bangmin
Deng, Fengli
Perry, Seth W.
Wong, Ma‐Li
Licinio, Julio
Wei, Hong
Yu, Gang
Xie, Peng
author_sort Zheng, Peng
collection PubMed
description Myasthenia gravis (MG) is a devastating acquired autoimmune disease. Emerging evidence indicates that the gut microbiome plays a key role in maintaining immune system homeostasis. This work reports that MG is characterized by decreased α‐phylogenetic diversity, and significantly disturbed gut microbiome and fecal metabolome. The altered gut microbial composition is associated with fecal metabolome changes, with 38.75% of altered bacterial operational taxonomic units showing significant correlations with a range of metabolite biomarkers. Some microbes are particularly linked with MG severity. Moreover, a combination of microbial makers and their correlated metabolites enable discriminating MG from healthy controls (HCs) with 100% accuracy. To investigate whether disturbed gut mcirobiome might contribute to the onset of MG, germ‐free (GF) mice are initially colonized with MG microbiota (MMb) or healthy microbiota (HMb), and then immunized in a classic mouse model of MG. The MMb mice demonstrate substantially impaired locomotion ability compared with the HMb mice. This effect could be reversed by cocolonizing GF mice with both MMb and HMb. The MMb mice also exhibit similar disturbances of fecal metabolic pathways as found in MG. Together these data demonstrate disturbances in microbiome composition and activity that are likely to be relevant to the pathogenesis of MG.
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spelling pubmed-67555402019-09-26 Perturbed Microbial Ecology in Myasthenia Gravis: Evidence from the Gut Microbiome and Fecal Metabolome Zheng, Peng Li, Yifan Wu, Jing Zhang, Hanping Huang, Yu Tan, Xunmin Pan, Junxi Duan, Jiajia Liang, Weiwei Yin, Bangmin Deng, Fengli Perry, Seth W. Wong, Ma‐Li Licinio, Julio Wei, Hong Yu, Gang Xie, Peng Adv Sci (Weinh) Full Papers Myasthenia gravis (MG) is a devastating acquired autoimmune disease. Emerging evidence indicates that the gut microbiome plays a key role in maintaining immune system homeostasis. This work reports that MG is characterized by decreased α‐phylogenetic diversity, and significantly disturbed gut microbiome and fecal metabolome. The altered gut microbial composition is associated with fecal metabolome changes, with 38.75% of altered bacterial operational taxonomic units showing significant correlations with a range of metabolite biomarkers. Some microbes are particularly linked with MG severity. Moreover, a combination of microbial makers and their correlated metabolites enable discriminating MG from healthy controls (HCs) with 100% accuracy. To investigate whether disturbed gut mcirobiome might contribute to the onset of MG, germ‐free (GF) mice are initially colonized with MG microbiota (MMb) or healthy microbiota (HMb), and then immunized in a classic mouse model of MG. The MMb mice demonstrate substantially impaired locomotion ability compared with the HMb mice. This effect could be reversed by cocolonizing GF mice with both MMb and HMb. The MMb mice also exhibit similar disturbances of fecal metabolic pathways as found in MG. Together these data demonstrate disturbances in microbiome composition and activity that are likely to be relevant to the pathogenesis of MG. John Wiley and Sons Inc. 2019-07-31 /pmc/articles/PMC6755540/ /pubmed/31559142 http://dx.doi.org/10.1002/advs.201901441 Text en © 2019 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Full Papers
Zheng, Peng
Li, Yifan
Wu, Jing
Zhang, Hanping
Huang, Yu
Tan, Xunmin
Pan, Junxi
Duan, Jiajia
Liang, Weiwei
Yin, Bangmin
Deng, Fengli
Perry, Seth W.
Wong, Ma‐Li
Licinio, Julio
Wei, Hong
Yu, Gang
Xie, Peng
Perturbed Microbial Ecology in Myasthenia Gravis: Evidence from the Gut Microbiome and Fecal Metabolome
title Perturbed Microbial Ecology in Myasthenia Gravis: Evidence from the Gut Microbiome and Fecal Metabolome
title_full Perturbed Microbial Ecology in Myasthenia Gravis: Evidence from the Gut Microbiome and Fecal Metabolome
title_fullStr Perturbed Microbial Ecology in Myasthenia Gravis: Evidence from the Gut Microbiome and Fecal Metabolome
title_full_unstemmed Perturbed Microbial Ecology in Myasthenia Gravis: Evidence from the Gut Microbiome and Fecal Metabolome
title_short Perturbed Microbial Ecology in Myasthenia Gravis: Evidence from the Gut Microbiome and Fecal Metabolome
title_sort perturbed microbial ecology in myasthenia gravis: evidence from the gut microbiome and fecal metabolome
topic Full Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6755540/
https://www.ncbi.nlm.nih.gov/pubmed/31559142
http://dx.doi.org/10.1002/advs.201901441
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