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CNDP2 Acts as an Activator for Human Ovarian Cancer Growth and Metastasis via the PI3K/AKT Pathway
INTRODUCTION: The mechanism of tumorigenesis and metastasis of ovarian cancer has not yet been elucidated. This study aimed to investigate the role and molecular mechanism of cytosolic nonspecific dipeptidase 2 in tumorigenesis and metastasis. METHODS: Cytosolic nonspecific dipeptidase 2 expression...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6755628/ https://www.ncbi.nlm.nih.gov/pubmed/31537175 http://dx.doi.org/10.1177/1533033819874773 |
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author | Zhang, Li Q. Yang, Hua Q. Yang, Su Q. Wang, Ying Chen, Xian J. Lu, Hong S. Zhao, Ling P. |
author_facet | Zhang, Li Q. Yang, Hua Q. Yang, Su Q. Wang, Ying Chen, Xian J. Lu, Hong S. Zhao, Ling P. |
author_sort | Zhang, Li Q. |
collection | PubMed |
description | INTRODUCTION: The mechanism of tumorigenesis and metastasis of ovarian cancer has not yet been elucidated. This study aimed to investigate the role and molecular mechanism of cytosolic nonspecific dipeptidase 2 in tumorigenesis and metastasis. METHODS: Cytosolic nonspecific dipeptidase 2 expression in human ovarian cancer tissues and cell lines was assessed with methyl thiazolyl tetrazolium (MTT), clone formation, and transwell assays performed to evaluate the ability of ovarian cancer cells to proliferate and migrate. Nude mice tumor formation experiments were also performed by subcutaneously injecting cells with stable cytosolic nonspecific dipeptidase 2 knockdown and control SKOV3 cells into BALB/c female nude mice to detect changes in PI3K/AKT pathway-related proteins by Western blotting. RESULTS: Cytosolic nonspecific dipeptidase 2 was highly expressed in human ovarian cancer tissues, with its expression associated with pathological data, including ovarian cancer metastasis. A cytosolic nonspecific dipeptidase 2 stable knockdown or ectopic expression ovarian cancer cell model was established and demonstrated that cytosolic nonspecific dipeptidase 2 could promote the proliferation of ovarian cancer cells. Transwell cell migration and invasion assays confirmed that cytosolic nonspecific dipeptidase 2 enhanced cell metastasis in ovarian cancer. Furthermore, in vivo xenograft experiments demonstrated that cytosolic nonspecific dipeptidase 2 can promote the development and progression of ovarian cancer, increasing the expression of phosphorylated PI3K and AKT. CONCLUSIONS: Cytosolic nonspecific dipeptidase 2 promotes the occurrence and development of ovarian cancer through the PI3K/AKT signaling pathway. |
format | Online Article Text |
id | pubmed-6755628 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-67556282019-09-27 CNDP2 Acts as an Activator for Human Ovarian Cancer Growth and Metastasis via the PI3K/AKT Pathway Zhang, Li Q. Yang, Hua Q. Yang, Su Q. Wang, Ying Chen, Xian J. Lu, Hong S. Zhao, Ling P. Technol Cancer Res Treat Original Article INTRODUCTION: The mechanism of tumorigenesis and metastasis of ovarian cancer has not yet been elucidated. This study aimed to investigate the role and molecular mechanism of cytosolic nonspecific dipeptidase 2 in tumorigenesis and metastasis. METHODS: Cytosolic nonspecific dipeptidase 2 expression in human ovarian cancer tissues and cell lines was assessed with methyl thiazolyl tetrazolium (MTT), clone formation, and transwell assays performed to evaluate the ability of ovarian cancer cells to proliferate and migrate. Nude mice tumor formation experiments were also performed by subcutaneously injecting cells with stable cytosolic nonspecific dipeptidase 2 knockdown and control SKOV3 cells into BALB/c female nude mice to detect changes in PI3K/AKT pathway-related proteins by Western blotting. RESULTS: Cytosolic nonspecific dipeptidase 2 was highly expressed in human ovarian cancer tissues, with its expression associated with pathological data, including ovarian cancer metastasis. A cytosolic nonspecific dipeptidase 2 stable knockdown or ectopic expression ovarian cancer cell model was established and demonstrated that cytosolic nonspecific dipeptidase 2 could promote the proliferation of ovarian cancer cells. Transwell cell migration and invasion assays confirmed that cytosolic nonspecific dipeptidase 2 enhanced cell metastasis in ovarian cancer. Furthermore, in vivo xenograft experiments demonstrated that cytosolic nonspecific dipeptidase 2 can promote the development and progression of ovarian cancer, increasing the expression of phosphorylated PI3K and AKT. CONCLUSIONS: Cytosolic nonspecific dipeptidase 2 promotes the occurrence and development of ovarian cancer through the PI3K/AKT signaling pathway. SAGE Publications 2019-09-19 /pmc/articles/PMC6755628/ /pubmed/31537175 http://dx.doi.org/10.1177/1533033819874773 Text en © The Author(s) 2019 http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Article Zhang, Li Q. Yang, Hua Q. Yang, Su Q. Wang, Ying Chen, Xian J. Lu, Hong S. Zhao, Ling P. CNDP2 Acts as an Activator for Human Ovarian Cancer Growth and Metastasis via the PI3K/AKT Pathway |
title | CNDP2 Acts as an Activator for Human Ovarian Cancer Growth and Metastasis via
the PI3K/AKT Pathway |
title_full | CNDP2 Acts as an Activator for Human Ovarian Cancer Growth and Metastasis via
the PI3K/AKT Pathway |
title_fullStr | CNDP2 Acts as an Activator for Human Ovarian Cancer Growth and Metastasis via
the PI3K/AKT Pathway |
title_full_unstemmed | CNDP2 Acts as an Activator for Human Ovarian Cancer Growth and Metastasis via
the PI3K/AKT Pathway |
title_short | CNDP2 Acts as an Activator for Human Ovarian Cancer Growth and Metastasis via
the PI3K/AKT Pathway |
title_sort | cndp2 acts as an activator for human ovarian cancer growth and metastasis via
the pi3k/akt pathway |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6755628/ https://www.ncbi.nlm.nih.gov/pubmed/31537175 http://dx.doi.org/10.1177/1533033819874773 |
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