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Polar Effects of Transposon Insertion into a Minimal Bacterial Genome

Global transposon mutagenesis is a valuable tool for identifying genes required for cell viability. Here we present a global analysis of the orientation of viable Tn5-Puro(r) (Tn5-puromycin resistance) insertions into the near-minimal bacterial genome of JCVI-syn2.0. Sixteen of the 478 protein-codin...

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Detalles Bibliográficos
Autores principales:	Hutchison, Clyde A., Merryman, Chuck, Sun, Lijie, Assad-Garcia, Nacyra, Richter, R. Alexander, Smith, Hamilton O., Glass, John I.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Microbiology 2019
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6755753/ https://www.ncbi.nlm.nih.gov/pubmed/31262838 http://dx.doi.org/10.1128/JB.00185-19

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