Cargando…
Muscone Promotes The Adipogenic Differentiation Of Human Gingival Mesenchymal Stem Cells By Inhibiting The Wnt/β-Catenin Signaling Pathway
OBJECTIVES: This study was performed to evaluate the effects of muscone on the proliferation, migration and differentiation of human gingival mesenchymal stem cells (GMSCs) and to explore the relevant mechanisms. MATERIALS AND METHODS: We performed studies to determine the effects and mechanisms of...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6756161/ https://www.ncbi.nlm.nih.gov/pubmed/31571831 http://dx.doi.org/10.2147/DDDT.S220970 |
_version_ | 1783453359513010176 |
---|---|
author | Yuan, Wen-Xiu Wang, Xu-Xia Zheng, De-Hua Ma, Dan Cui, Qun Yang, Fan Zhang, Jun |
author_facet | Yuan, Wen-Xiu Wang, Xu-Xia Zheng, De-Hua Ma, Dan Cui, Qun Yang, Fan Zhang, Jun |
author_sort | Yuan, Wen-Xiu |
collection | PubMed |
description | OBJECTIVES: This study was performed to evaluate the effects of muscone on the proliferation, migration and differentiation of human gingival mesenchymal stem cells (GMSCs) and to explore the relevant mechanisms. MATERIALS AND METHODS: We performed studies to determine the effects and mechanisms of muscone on GMSC proliferation, migration and differentiation. We conducted CCK-8, colony formation, transwell chamber, scratch wound, alkaline phosphatase (ALP) staining and activity, and alizarin red and oil red O staining assays, as well as real-time quantitative polymerase chain reaction (qRT-PCR), to ascertain the effects of muscone on GMSC proliferation, migration and differentiation in vitro. The mechanism by which muscone influences the osteogenic and adipogenic differentiation of GMSCs was elucidated by qRT-PCR and Western blotting. RESULTS: We found that muscone significantly promoted GMSC proliferation, chemotaxis, wound healing and fat droplet formation and inhibited ALP activity and mineral deposition. Notably, we observed that the Wnt/β-catenin pathway was closely related to the ability of muscone to inhibit the osteogenic differentiation and promote the adipogenic differentiation of GMSCs. The effect of muscone on the multidirectional differentiation capacity of GMSCs was significantly reversed by the agonist lithium chloride through the Wnt/β-catenin signaling pathway. CONCLUSION: Muscone effectively increased the proliferation and migration, promoted the adipogenic differentiation and inhibited the osteogenic differentiation of GMSCs by inhibiting the Wnt/β-catenin signaling pathway. These results may provide a theoretical basis for the application of GMSCs and muscone in tissue engineering and regenerative medicine. |
format | Online Article Text |
id | pubmed-6756161 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-67561612019-09-30 Muscone Promotes The Adipogenic Differentiation Of Human Gingival Mesenchymal Stem Cells By Inhibiting The Wnt/β-Catenin Signaling Pathway Yuan, Wen-Xiu Wang, Xu-Xia Zheng, De-Hua Ma, Dan Cui, Qun Yang, Fan Zhang, Jun Drug Des Devel Ther Original Research OBJECTIVES: This study was performed to evaluate the effects of muscone on the proliferation, migration and differentiation of human gingival mesenchymal stem cells (GMSCs) and to explore the relevant mechanisms. MATERIALS AND METHODS: We performed studies to determine the effects and mechanisms of muscone on GMSC proliferation, migration and differentiation. We conducted CCK-8, colony formation, transwell chamber, scratch wound, alkaline phosphatase (ALP) staining and activity, and alizarin red and oil red O staining assays, as well as real-time quantitative polymerase chain reaction (qRT-PCR), to ascertain the effects of muscone on GMSC proliferation, migration and differentiation in vitro. The mechanism by which muscone influences the osteogenic and adipogenic differentiation of GMSCs was elucidated by qRT-PCR and Western blotting. RESULTS: We found that muscone significantly promoted GMSC proliferation, chemotaxis, wound healing and fat droplet formation and inhibited ALP activity and mineral deposition. Notably, we observed that the Wnt/β-catenin pathway was closely related to the ability of muscone to inhibit the osteogenic differentiation and promote the adipogenic differentiation of GMSCs. The effect of muscone on the multidirectional differentiation capacity of GMSCs was significantly reversed by the agonist lithium chloride through the Wnt/β-catenin signaling pathway. CONCLUSION: Muscone effectively increased the proliferation and migration, promoted the adipogenic differentiation and inhibited the osteogenic differentiation of GMSCs by inhibiting the Wnt/β-catenin signaling pathway. These results may provide a theoretical basis for the application of GMSCs and muscone in tissue engineering and regenerative medicine. Dove 2019-09-18 /pmc/articles/PMC6756161/ /pubmed/31571831 http://dx.doi.org/10.2147/DDDT.S220970 Text en © 2019 Yuan et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Yuan, Wen-Xiu Wang, Xu-Xia Zheng, De-Hua Ma, Dan Cui, Qun Yang, Fan Zhang, Jun Muscone Promotes The Adipogenic Differentiation Of Human Gingival Mesenchymal Stem Cells By Inhibiting The Wnt/β-Catenin Signaling Pathway |
title | Muscone Promotes The Adipogenic Differentiation Of Human Gingival Mesenchymal Stem Cells By Inhibiting The Wnt/β-Catenin Signaling Pathway |
title_full | Muscone Promotes The Adipogenic Differentiation Of Human Gingival Mesenchymal Stem Cells By Inhibiting The Wnt/β-Catenin Signaling Pathway |
title_fullStr | Muscone Promotes The Adipogenic Differentiation Of Human Gingival Mesenchymal Stem Cells By Inhibiting The Wnt/β-Catenin Signaling Pathway |
title_full_unstemmed | Muscone Promotes The Adipogenic Differentiation Of Human Gingival Mesenchymal Stem Cells By Inhibiting The Wnt/β-Catenin Signaling Pathway |
title_short | Muscone Promotes The Adipogenic Differentiation Of Human Gingival Mesenchymal Stem Cells By Inhibiting The Wnt/β-Catenin Signaling Pathway |
title_sort | muscone promotes the adipogenic differentiation of human gingival mesenchymal stem cells by inhibiting the wnt/β-catenin signaling pathway |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6756161/ https://www.ncbi.nlm.nih.gov/pubmed/31571831 http://dx.doi.org/10.2147/DDDT.S220970 |
work_keys_str_mv | AT yuanwenxiu musconepromotestheadipogenicdifferentiationofhumangingivalmesenchymalstemcellsbyinhibitingthewntbcateninsignalingpathway AT wangxuxia musconepromotestheadipogenicdifferentiationofhumangingivalmesenchymalstemcellsbyinhibitingthewntbcateninsignalingpathway AT zhengdehua musconepromotestheadipogenicdifferentiationofhumangingivalmesenchymalstemcellsbyinhibitingthewntbcateninsignalingpathway AT madan musconepromotestheadipogenicdifferentiationofhumangingivalmesenchymalstemcellsbyinhibitingthewntbcateninsignalingpathway AT cuiqun musconepromotestheadipogenicdifferentiationofhumangingivalmesenchymalstemcellsbyinhibitingthewntbcateninsignalingpathway AT yangfan musconepromotestheadipogenicdifferentiationofhumangingivalmesenchymalstemcellsbyinhibitingthewntbcateninsignalingpathway AT zhangjun musconepromotestheadipogenicdifferentiationofhumangingivalmesenchymalstemcellsbyinhibitingthewntbcateninsignalingpathway |