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High-throughput Sequencing-based Analysis of the Intestinal Microbiota of Broiler Chickens Fed Genetically Modified Rice Expressing Cry1Ac/Cry1Ab Chimeric Bacillus thuringiensis Protein

Many types of Bacillus thuringiensis (Bt)-crops are being grown worldwide, triggering concerns about their potential impact on humans and livestock. To ensure better yield and food safety in China, an attempt has been made to develop Bt-rice targeting a broad range of insects. We aimed to investigat...

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Autores principales: Lili, Geng, Deng, Xu, Minhong, Zhang, Changlong, Shu, Jinghai, Feng, Fuping, Song, Fan, Lu, Jie, Zhang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japan Poultry Science Association 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6756379/
https://www.ncbi.nlm.nih.gov/pubmed/32055151
http://dx.doi.org/10.2141/jpsa.0170029
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author Lili, Geng
Deng, Xu
Minhong, Zhang
Changlong, Shu
Jinghai, Feng
Fuping, Song
Fan, Lu
Jie, Zhang
author_facet Lili, Geng
Deng, Xu
Minhong, Zhang
Changlong, Shu
Jinghai, Feng
Fuping, Song
Fan, Lu
Jie, Zhang
author_sort Lili, Geng
collection PubMed
description Many types of Bacillus thuringiensis (Bt)-crops are being grown worldwide, triggering concerns about their potential impact on humans and livestock. To ensure better yield and food safety in China, an attempt has been made to develop Bt-rice targeting a broad range of insects. We aimed to investigate whether feeding genetically modified rice expressing the Bt chimeric Cry1Ac/Cry1Ab protein has any effects on the intestinal microbiota of broilers. Broilers were fed either Bt-rice or its unmodified isogenic parent line for 42 days, and total DNA was isolated from cecum contents for high-throughput sequencing of the 16S rRNA gene. In total, 1,241,005 reads, assigned to 12 phyla, 31 families, and 48 genera were generated. No significant differences were observed in the relative abundance of organisms identified among the major phyla, families, and genera, except for two less abundant families, Thermoanaerobacteraceae and Peptostreptococcaceae, and two less abundant genera, Anaerotruncus and Gelria. The results were in agreement with those from culture-based analysis and Biolog EcoPlates. These results illustrate that feeding Bt-rice has no adverse effects on the broiler intestinal microbiota and provide sufficient support for the food safety of Bt-rice.
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spelling pubmed-67563792020-02-13 High-throughput Sequencing-based Analysis of the Intestinal Microbiota of Broiler Chickens Fed Genetically Modified Rice Expressing Cry1Ac/Cry1Ab Chimeric Bacillus thuringiensis Protein Lili, Geng Deng, Xu Minhong, Zhang Changlong, Shu Jinghai, Feng Fuping, Song Fan, Lu Jie, Zhang J Poult Sci Original Papers Many types of Bacillus thuringiensis (Bt)-crops are being grown worldwide, triggering concerns about their potential impact on humans and livestock. To ensure better yield and food safety in China, an attempt has been made to develop Bt-rice targeting a broad range of insects. We aimed to investigate whether feeding genetically modified rice expressing the Bt chimeric Cry1Ac/Cry1Ab protein has any effects on the intestinal microbiota of broilers. Broilers were fed either Bt-rice or its unmodified isogenic parent line for 42 days, and total DNA was isolated from cecum contents for high-throughput sequencing of the 16S rRNA gene. In total, 1,241,005 reads, assigned to 12 phyla, 31 families, and 48 genera were generated. No significant differences were observed in the relative abundance of organisms identified among the major phyla, families, and genera, except for two less abundant families, Thermoanaerobacteraceae and Peptostreptococcaceae, and two less abundant genera, Anaerotruncus and Gelria. The results were in agreement with those from culture-based analysis and Biolog EcoPlates. These results illustrate that feeding Bt-rice has no adverse effects on the broiler intestinal microbiota and provide sufficient support for the food safety of Bt-rice. Japan Poultry Science Association 2018 2017-08-25 /pmc/articles/PMC6756379/ /pubmed/32055151 http://dx.doi.org/10.2141/jpsa.0170029 Text en 2018 by Japan Poultry Science Association https://creativecommons.org/licenses/by-nc-sa/4.0/ The Journal of Poultry Science is an Open Access journal distributed under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. To view the details of this license, please visit (https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Original Papers
Lili, Geng
Deng, Xu
Minhong, Zhang
Changlong, Shu
Jinghai, Feng
Fuping, Song
Fan, Lu
Jie, Zhang
High-throughput Sequencing-based Analysis of the Intestinal Microbiota of Broiler Chickens Fed Genetically Modified Rice Expressing Cry1Ac/Cry1Ab Chimeric Bacillus thuringiensis Protein
title High-throughput Sequencing-based Analysis of the Intestinal Microbiota of Broiler Chickens Fed Genetically Modified Rice Expressing Cry1Ac/Cry1Ab Chimeric Bacillus thuringiensis Protein
title_full High-throughput Sequencing-based Analysis of the Intestinal Microbiota of Broiler Chickens Fed Genetically Modified Rice Expressing Cry1Ac/Cry1Ab Chimeric Bacillus thuringiensis Protein
title_fullStr High-throughput Sequencing-based Analysis of the Intestinal Microbiota of Broiler Chickens Fed Genetically Modified Rice Expressing Cry1Ac/Cry1Ab Chimeric Bacillus thuringiensis Protein
title_full_unstemmed High-throughput Sequencing-based Analysis of the Intestinal Microbiota of Broiler Chickens Fed Genetically Modified Rice Expressing Cry1Ac/Cry1Ab Chimeric Bacillus thuringiensis Protein
title_short High-throughput Sequencing-based Analysis of the Intestinal Microbiota of Broiler Chickens Fed Genetically Modified Rice Expressing Cry1Ac/Cry1Ab Chimeric Bacillus thuringiensis Protein
title_sort high-throughput sequencing-based analysis of the intestinal microbiota of broiler chickens fed genetically modified rice expressing cry1ac/cry1ab chimeric bacillus thuringiensis protein
topic Original Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6756379/
https://www.ncbi.nlm.nih.gov/pubmed/32055151
http://dx.doi.org/10.2141/jpsa.0170029
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