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Isolation, identification and characterization of Streptomyces metabolites as a potential bioherbicide

Bioactive herbicidal compounds produced by soil microorganisms might be used to creating a bioherbicide for biological weed control. A total of 1,300 bacterial strains were isolated and screened for herbicidal activity against grass and broadleaf weeds. Among primarily selected 102 strains, the herb...

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Autores principales: Bo, Aung B., Kim, Jae D., Kim, Young S., Sin, Hun T., Kim, Hye J., Khaitov, Botir, Ko, Young K., Park, Kee W., Choi, Jung S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6756554/
https://www.ncbi.nlm.nih.gov/pubmed/31545849
http://dx.doi.org/10.1371/journal.pone.0222933
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author Bo, Aung B.
Kim, Jae D.
Kim, Young S.
Sin, Hun T.
Kim, Hye J.
Khaitov, Botir
Ko, Young K.
Park, Kee W.
Choi, Jung S.
author_facet Bo, Aung B.
Kim, Jae D.
Kim, Young S.
Sin, Hun T.
Kim, Hye J.
Khaitov, Botir
Ko, Young K.
Park, Kee W.
Choi, Jung S.
author_sort Bo, Aung B.
collection PubMed
description Bioactive herbicidal compounds produced by soil microorganisms might be used to creating a bioherbicide for biological weed control. A total of 1,300 bacterial strains were isolated and screened for herbicidal activity against grass and broadleaf weeds. Among primarily selected 102 strains, the herbicidal activity of bacterial fermentation broths from the following three isolates strain-101, strain-128, and strain-329 reduced the growth of D. sanguinalis by 66.7%, 78.3%, and 100%, respectively as compared with control. Phylogenetic analysis of 16S rRNA gene sequencing determined that the strain-329 has 99% similarity to Streptomyces anulatus (HBUM 174206). The potential bioherbicidal efficacy of Streptomyces strain-329 was tested on grass and broadleaf weeds for phytotoxic activity through pre- and post-emergence applications. At pre-emergence application, the phytotoxic efficacy to D. sanguinalis and S. bicolor on seed germination were 90.4% and 81.3%, respectively at the 2x concentration, whereas in the case of Solanum nigrum, 85.2% phytotoxic efficacy was observed at the 4x concentration. The efficacy of Streptomyces strain-329 was substantially higher at post-emergence application, presenting 100% control of grass and broadleaf weeds at the 1x concentration. Two herbicidal compounds coded as 329-C1 and 329-C3 were extracted and purified by column chromatography and high-performance liquid chromatography methods. The active compound 329-C3 slightly increased leaf electrolytic leakage and MDA production as concentration-dependent manner. These results suggest that new Streptomyces sp. strain-329 produced bioherbicidal metabolites and may provide a new lead molecule for production an efficient bioherbicide to regulate grass and broadleaf weeds.
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spelling pubmed-67565542019-10-04 Isolation, identification and characterization of Streptomyces metabolites as a potential bioherbicide Bo, Aung B. Kim, Jae D. Kim, Young S. Sin, Hun T. Kim, Hye J. Khaitov, Botir Ko, Young K. Park, Kee W. Choi, Jung S. PLoS One Research Article Bioactive herbicidal compounds produced by soil microorganisms might be used to creating a bioherbicide for biological weed control. A total of 1,300 bacterial strains were isolated and screened for herbicidal activity against grass and broadleaf weeds. Among primarily selected 102 strains, the herbicidal activity of bacterial fermentation broths from the following three isolates strain-101, strain-128, and strain-329 reduced the growth of D. sanguinalis by 66.7%, 78.3%, and 100%, respectively as compared with control. Phylogenetic analysis of 16S rRNA gene sequencing determined that the strain-329 has 99% similarity to Streptomyces anulatus (HBUM 174206). The potential bioherbicidal efficacy of Streptomyces strain-329 was tested on grass and broadleaf weeds for phytotoxic activity through pre- and post-emergence applications. At pre-emergence application, the phytotoxic efficacy to D. sanguinalis and S. bicolor on seed germination were 90.4% and 81.3%, respectively at the 2x concentration, whereas in the case of Solanum nigrum, 85.2% phytotoxic efficacy was observed at the 4x concentration. The efficacy of Streptomyces strain-329 was substantially higher at post-emergence application, presenting 100% control of grass and broadleaf weeds at the 1x concentration. Two herbicidal compounds coded as 329-C1 and 329-C3 were extracted and purified by column chromatography and high-performance liquid chromatography methods. The active compound 329-C3 slightly increased leaf electrolytic leakage and MDA production as concentration-dependent manner. These results suggest that new Streptomyces sp. strain-329 produced bioherbicidal metabolites and may provide a new lead molecule for production an efficient bioherbicide to regulate grass and broadleaf weeds. Public Library of Science 2019-09-23 /pmc/articles/PMC6756554/ /pubmed/31545849 http://dx.doi.org/10.1371/journal.pone.0222933 Text en © 2019 Bo et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Bo, Aung B.
Kim, Jae D.
Kim, Young S.
Sin, Hun T.
Kim, Hye J.
Khaitov, Botir
Ko, Young K.
Park, Kee W.
Choi, Jung S.
Isolation, identification and characterization of Streptomyces metabolites as a potential bioherbicide
title Isolation, identification and characterization of Streptomyces metabolites as a potential bioherbicide
title_full Isolation, identification and characterization of Streptomyces metabolites as a potential bioherbicide
title_fullStr Isolation, identification and characterization of Streptomyces metabolites as a potential bioherbicide
title_full_unstemmed Isolation, identification and characterization of Streptomyces metabolites as a potential bioherbicide
title_short Isolation, identification and characterization of Streptomyces metabolites as a potential bioherbicide
title_sort isolation, identification and characterization of streptomyces metabolites as a potential bioherbicide
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6756554/
https://www.ncbi.nlm.nih.gov/pubmed/31545849
http://dx.doi.org/10.1371/journal.pone.0222933
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