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Engineering Photorhabdus luminescens toxin complex (PTC) into a recombinant injection nanomachine
Engineering delivery systems for proteins and peptides into mammalian cells is an ongoing challenge for cell biological studies as well as for therapeutic approaches. Photorhabdus luminescens toxin complex (PTC) is a heterotrimeric protein complex able to deliver diverse protein toxins into mammalia...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Life Science Alliance LLC
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6756610/ https://www.ncbi.nlm.nih.gov/pubmed/31540947 http://dx.doi.org/10.26508/lsa.201900485 |
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author | Ng´ang´a, Peter Njenga Ebner, Julia K Plessner, Matthias Aktories, Klaus Schmidt, Gudula |
author_facet | Ng´ang´a, Peter Njenga Ebner, Julia K Plessner, Matthias Aktories, Klaus Schmidt, Gudula |
author_sort | Ng´ang´a, Peter Njenga |
collection | PubMed |
description | Engineering delivery systems for proteins and peptides into mammalian cells is an ongoing challenge for cell biological studies as well as for therapeutic approaches. Photorhabdus luminescens toxin complex (PTC) is a heterotrimeric protein complex able to deliver diverse protein toxins into mammalian cells. We engineered the syringe-like nanomachine for delivery of protein toxins from different species. In addition, we loaded the highly active copepod luciferase Metridia longa M-Luc7 for accurate quantification of injected molecules. We suggest that besides the probable size limitation, the charge of the cargo also influences the efficiency of packing and transport into mammalian cells. Our data show that the PTC constitutes a powerful system to inject recombinant proteins, peptides, and potentially, other molecules into mammalian cells. In addition, in contrast to other protein transporters based on pore formation, the closed, compact structure of the PTC may protect cargo from degradation. |
format | Online Article Text |
id | pubmed-6756610 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Life Science Alliance LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-67566102019-10-02 Engineering Photorhabdus luminescens toxin complex (PTC) into a recombinant injection nanomachine Ng´ang´a, Peter Njenga Ebner, Julia K Plessner, Matthias Aktories, Klaus Schmidt, Gudula Life Sci Alliance Research Articles Engineering delivery systems for proteins and peptides into mammalian cells is an ongoing challenge for cell biological studies as well as for therapeutic approaches. Photorhabdus luminescens toxin complex (PTC) is a heterotrimeric protein complex able to deliver diverse protein toxins into mammalian cells. We engineered the syringe-like nanomachine for delivery of protein toxins from different species. In addition, we loaded the highly active copepod luciferase Metridia longa M-Luc7 for accurate quantification of injected molecules. We suggest that besides the probable size limitation, the charge of the cargo also influences the efficiency of packing and transport into mammalian cells. Our data show that the PTC constitutes a powerful system to inject recombinant proteins, peptides, and potentially, other molecules into mammalian cells. In addition, in contrast to other protein transporters based on pore formation, the closed, compact structure of the PTC may protect cargo from degradation. Life Science Alliance LLC 2019-09-20 /pmc/articles/PMC6756610/ /pubmed/31540947 http://dx.doi.org/10.26508/lsa.201900485 Text en © 2019 Ng´ang´a et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Articles Ng´ang´a, Peter Njenga Ebner, Julia K Plessner, Matthias Aktories, Klaus Schmidt, Gudula Engineering Photorhabdus luminescens toxin complex (PTC) into a recombinant injection nanomachine |
title | Engineering Photorhabdus luminescens toxin complex (PTC) into a recombinant injection nanomachine |
title_full | Engineering Photorhabdus luminescens toxin complex (PTC) into a recombinant injection nanomachine |
title_fullStr | Engineering Photorhabdus luminescens toxin complex (PTC) into a recombinant injection nanomachine |
title_full_unstemmed | Engineering Photorhabdus luminescens toxin complex (PTC) into a recombinant injection nanomachine |
title_short | Engineering Photorhabdus luminescens toxin complex (PTC) into a recombinant injection nanomachine |
title_sort | engineering photorhabdus luminescens toxin complex (ptc) into a recombinant injection nanomachine |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6756610/ https://www.ncbi.nlm.nih.gov/pubmed/31540947 http://dx.doi.org/10.26508/lsa.201900485 |
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