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Circulating serum exosomal aHIF is a novel prognostic predictor for epithelial ovarian cancer
PURPOSE: Exosomes are key mediators of cellular communication by transporting molecules, including long noncoding RNAs (lncRNAs), and have been regarded as promising non-invasive biomarkers. This study aimed to evaluate the expression pattern and clinical significance of serum exosomal lncRNA antise...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6756917/ https://www.ncbi.nlm.nih.gov/pubmed/31571921 http://dx.doi.org/10.2147/OTT.S220533 |
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author | Tang, Xiaoyan Liu, Songping Liu, Yinglei Lin, Xiaojing Zheng, Tingting Liu, Xin Qiu, Junjun Hua, Keqin |
author_facet | Tang, Xiaoyan Liu, Songping Liu, Yinglei Lin, Xiaojing Zheng, Tingting Liu, Xin Qiu, Junjun Hua, Keqin |
author_sort | Tang, Xiaoyan |
collection | PubMed |
description | PURPOSE: Exosomes are key mediators of cellular communication by transporting molecules, including long noncoding RNAs (lncRNAs), and have been regarded as promising non-invasive biomarkers. This study aimed to evaluate the expression pattern and clinical significance of serum exosomal lncRNA antisense hypoxia inducible factor (aHIF) in epithelial ovarian cancer (EOC). PATIENTS AND METHODS: Sixty-two EOC patients in Obstetrics and Gynecology Hospital of Fudan University were enrolled. The expression levels of aHIF in tissues and serum exosomes were examined by RT-qPCR. The origin of serum exosomal aHIF was explored in vitro and in vivo. Univariate and multivariate Cox regression analyses were used to evaluate the prognostic factors of EOC. A prognostic predictive nomogram was formulated in R software. RESULTS: We isolated exosomes, identified exosomal aHIF in the serum of EOC patients. The expression of serum exosomal aHIF was higher in EOC patients and was correlated with the aHIF level in EOC tissues. In vitro and in vivo, the results indicated that serum exosomal aHIF was derived from tumor cells. Kaplan-Meier survival analysis demonstrated that EOC patients with higher serum exosomal aHIF expression had poorer overall survival. Cox multivariate regression model revealed that FIGO stage, residual tumor size, and serum exosomal aHIF level were independent prognostic factors of EOC. Based on the prognostic value of serum exosomal aHIF, we established a nomogram model that showed a good predictive ability for EOC patients. CONCLUSION: Serum exosomal aHIF is overexpressed in EOC and can serve as a noninvasive predictive biomarker for unfavorable prognosis. |
format | Online Article Text |
id | pubmed-6756917 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-67569172019-09-30 Circulating serum exosomal aHIF is a novel prognostic predictor for epithelial ovarian cancer Tang, Xiaoyan Liu, Songping Liu, Yinglei Lin, Xiaojing Zheng, Tingting Liu, Xin Qiu, Junjun Hua, Keqin Onco Targets Ther Original Research PURPOSE: Exosomes are key mediators of cellular communication by transporting molecules, including long noncoding RNAs (lncRNAs), and have been regarded as promising non-invasive biomarkers. This study aimed to evaluate the expression pattern and clinical significance of serum exosomal lncRNA antisense hypoxia inducible factor (aHIF) in epithelial ovarian cancer (EOC). PATIENTS AND METHODS: Sixty-two EOC patients in Obstetrics and Gynecology Hospital of Fudan University were enrolled. The expression levels of aHIF in tissues and serum exosomes were examined by RT-qPCR. The origin of serum exosomal aHIF was explored in vitro and in vivo. Univariate and multivariate Cox regression analyses were used to evaluate the prognostic factors of EOC. A prognostic predictive nomogram was formulated in R software. RESULTS: We isolated exosomes, identified exosomal aHIF in the serum of EOC patients. The expression of serum exosomal aHIF was higher in EOC patients and was correlated with the aHIF level in EOC tissues. In vitro and in vivo, the results indicated that serum exosomal aHIF was derived from tumor cells. Kaplan-Meier survival analysis demonstrated that EOC patients with higher serum exosomal aHIF expression had poorer overall survival. Cox multivariate regression model revealed that FIGO stage, residual tumor size, and serum exosomal aHIF level were independent prognostic factors of EOC. Based on the prognostic value of serum exosomal aHIF, we established a nomogram model that showed a good predictive ability for EOC patients. CONCLUSION: Serum exosomal aHIF is overexpressed in EOC and can serve as a noninvasive predictive biomarker for unfavorable prognosis. Dove 2019-09-19 /pmc/articles/PMC6756917/ /pubmed/31571921 http://dx.doi.org/10.2147/OTT.S220533 Text en © 2019 Tang et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Tang, Xiaoyan Liu, Songping Liu, Yinglei Lin, Xiaojing Zheng, Tingting Liu, Xin Qiu, Junjun Hua, Keqin Circulating serum exosomal aHIF is a novel prognostic predictor for epithelial ovarian cancer |
title | Circulating serum exosomal aHIF is a novel prognostic predictor for epithelial ovarian cancer |
title_full | Circulating serum exosomal aHIF is a novel prognostic predictor for epithelial ovarian cancer |
title_fullStr | Circulating serum exosomal aHIF is a novel prognostic predictor for epithelial ovarian cancer |
title_full_unstemmed | Circulating serum exosomal aHIF is a novel prognostic predictor for epithelial ovarian cancer |
title_short | Circulating serum exosomal aHIF is a novel prognostic predictor for epithelial ovarian cancer |
title_sort | circulating serum exosomal ahif is a novel prognostic predictor for epithelial ovarian cancer |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6756917/ https://www.ncbi.nlm.nih.gov/pubmed/31571921 http://dx.doi.org/10.2147/OTT.S220533 |
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