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Resistance characteristics of CTX-M type Shigella flexneri in China
The present study was to identify the drug resistance, resistance mechanism and the extended-spectrum β-lactamase (ESBLs) genotypes of Shigella flexneri (S. flexneri) in Jinan. Susceptibility tests were performed by MIC-determination. The genotypes of β-lactamase were identified using PCR and DNA se...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6757185/ https://www.ncbi.nlm.nih.gov/pubmed/31519769 http://dx.doi.org/10.1042/BSR20191741 |
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author | Bian, Fengzhi Yao, Mingxiao Fu, Hongmei Yuan, Guangying Wu, Shuzhi Sun, Yuguo |
author_facet | Bian, Fengzhi Yao, Mingxiao Fu, Hongmei Yuan, Guangying Wu, Shuzhi Sun, Yuguo |
author_sort | Bian, Fengzhi |
collection | PubMed |
description | The present study was to identify the drug resistance, resistance mechanism and the extended-spectrum β-lactamase (ESBLs) genotypes of Shigella flexneri (S. flexneri) in Jinan. Susceptibility tests were performed by MIC-determination. The genotypes of β-lactamase were identified using PCR and DNA sequencing. The resistance transfer ability of the ESBL-producing strains was examined by conjugation tests. A total of 105 S. flexneri isolates were collected, and 34 (32.4%) were ESBL-producing isolates. All ESBL-producing isolates were susceptible to cefoxitin and imipenem, and 35.3% isolates were resistant to ciprofloxacin. ESBL-producing isolates showed high level resistant to ampicillin (100%), cefotaxime (100%), tetracycline (100%), chloramphenicol (100%), trimethoprim/sulfamethoxazole (100%), ceftazidime (73.5%) and cefepime (73.5%). Three types of β-lactamase genes (blaTEM, blaOXA and blaCTX-M) were identified in all ESBL-producing isolates, and the genotypes were confirmed as blaTEM-1 (23/34), blaOXA-30 (34/34), blaCTX-M-14 (9/34) and blaCTX-M-15 (25/34) by sequencing. In conclusion, the Shigella strains isolated in Jinan are cross-resistant and multi-drug resistant. The main genotypes of ESBLs are CTX-M-14 and CTX-M-15. |
format | Online Article Text |
id | pubmed-6757185 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-67571852019-10-02 Resistance characteristics of CTX-M type Shigella flexneri in China Bian, Fengzhi Yao, Mingxiao Fu, Hongmei Yuan, Guangying Wu, Shuzhi Sun, Yuguo Biosci Rep Research Articles The present study was to identify the drug resistance, resistance mechanism and the extended-spectrum β-lactamase (ESBLs) genotypes of Shigella flexneri (S. flexneri) in Jinan. Susceptibility tests were performed by MIC-determination. The genotypes of β-lactamase were identified using PCR and DNA sequencing. The resistance transfer ability of the ESBL-producing strains was examined by conjugation tests. A total of 105 S. flexneri isolates were collected, and 34 (32.4%) were ESBL-producing isolates. All ESBL-producing isolates were susceptible to cefoxitin and imipenem, and 35.3% isolates were resistant to ciprofloxacin. ESBL-producing isolates showed high level resistant to ampicillin (100%), cefotaxime (100%), tetracycline (100%), chloramphenicol (100%), trimethoprim/sulfamethoxazole (100%), ceftazidime (73.5%) and cefepime (73.5%). Three types of β-lactamase genes (blaTEM, blaOXA and blaCTX-M) were identified in all ESBL-producing isolates, and the genotypes were confirmed as blaTEM-1 (23/34), blaOXA-30 (34/34), blaCTX-M-14 (9/34) and blaCTX-M-15 (25/34) by sequencing. In conclusion, the Shigella strains isolated in Jinan are cross-resistant and multi-drug resistant. The main genotypes of ESBLs are CTX-M-14 and CTX-M-15. Portland Press Ltd. 2019-09-24 /pmc/articles/PMC6757185/ /pubmed/31519769 http://dx.doi.org/10.1042/BSR20191741 Text en © 2019 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Articles Bian, Fengzhi Yao, Mingxiao Fu, Hongmei Yuan, Guangying Wu, Shuzhi Sun, Yuguo Resistance characteristics of CTX-M type Shigella flexneri in China |
title | Resistance characteristics of CTX-M type Shigella flexneri in China |
title_full | Resistance characteristics of CTX-M type Shigella flexneri in China |
title_fullStr | Resistance characteristics of CTX-M type Shigella flexneri in China |
title_full_unstemmed | Resistance characteristics of CTX-M type Shigella flexneri in China |
title_short | Resistance characteristics of CTX-M type Shigella flexneri in China |
title_sort | resistance characteristics of ctx-m type shigella flexneri in china |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6757185/ https://www.ncbi.nlm.nih.gov/pubmed/31519769 http://dx.doi.org/10.1042/BSR20191741 |
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