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All-trans retinoic acid therapy induces asymmetric division of glioma stem cells from the U87MG cell line

The poor therapeutic effect of the current treatments for malignant glioma may be attributed to glioma stem cells (GSCs), which have been demonstrated to divide symmetrically. All-trans retinoic acid (ATRA)-induced differentiation is considered to target GSCs and has been reported to have the capabi...

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Detalles Bibliográficos
Autores principales: Wang, Ruizhi, Liu, Chongxiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6757269/
https://www.ncbi.nlm.nih.gov/pubmed/31579077
http://dx.doi.org/10.3892/ol.2019.10691
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author Wang, Ruizhi
Liu, Chongxiao
author_facet Wang, Ruizhi
Liu, Chongxiao
author_sort Wang, Ruizhi
collection PubMed
description The poor therapeutic effect of the current treatments for malignant glioma may be attributed to glioma stem cells (GSCs), which have been demonstrated to divide symmetrically. All-trans retinoic acid (ATRA)-induced differentiation is considered to target GSCs and has been reported to have the capability of eradicating cancer stem cells in specific malignancies. The aim of the present study was to investigate the effects of ATRA on the division mode of GSCs isolated from the U87MG glioblastoma cell line of unknown origin. The expressions of the GSC markers CD133 and nestin were detected using immunocytochemistry to identify GSCs. In addition, the differentiation potency of these GSCs was observed by detecting the expression of glial fibrillary acidic protein, β-tubulin III and galactosylceramidase using immunofluorescent staining. The Numb protein distribution was analyzed in two daughter cells following a GSC division. The results of the present study demonstrated that Numb protein is symmetrically segregated into two daughter cells during GSC division. Furthermore, the present study demonstrated that treatment with ATRA increased the asymmetric cell division of GSCs. In conclusion, these results suggest a therapeutic effect from ATRA-induced asymmetric division of GSCs from the U87MG cell line.
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spelling pubmed-67572692019-10-02 All-trans retinoic acid therapy induces asymmetric division of glioma stem cells from the U87MG cell line Wang, Ruizhi Liu, Chongxiao Oncol Lett Articles The poor therapeutic effect of the current treatments for malignant glioma may be attributed to glioma stem cells (GSCs), which have been demonstrated to divide symmetrically. All-trans retinoic acid (ATRA)-induced differentiation is considered to target GSCs and has been reported to have the capability of eradicating cancer stem cells in specific malignancies. The aim of the present study was to investigate the effects of ATRA on the division mode of GSCs isolated from the U87MG glioblastoma cell line of unknown origin. The expressions of the GSC markers CD133 and nestin were detected using immunocytochemistry to identify GSCs. In addition, the differentiation potency of these GSCs was observed by detecting the expression of glial fibrillary acidic protein, β-tubulin III and galactosylceramidase using immunofluorescent staining. The Numb protein distribution was analyzed in two daughter cells following a GSC division. The results of the present study demonstrated that Numb protein is symmetrically segregated into two daughter cells during GSC division. Furthermore, the present study demonstrated that treatment with ATRA increased the asymmetric cell division of GSCs. In conclusion, these results suggest a therapeutic effect from ATRA-induced asymmetric division of GSCs from the U87MG cell line. D.A. Spandidos 2019-10 2019-07-31 /pmc/articles/PMC6757269/ /pubmed/31579077 http://dx.doi.org/10.3892/ol.2019.10691 Text en Copyright: © Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Wang, Ruizhi
Liu, Chongxiao
All-trans retinoic acid therapy induces asymmetric division of glioma stem cells from the U87MG cell line
title All-trans retinoic acid therapy induces asymmetric division of glioma stem cells from the U87MG cell line
title_full All-trans retinoic acid therapy induces asymmetric division of glioma stem cells from the U87MG cell line
title_fullStr All-trans retinoic acid therapy induces asymmetric division of glioma stem cells from the U87MG cell line
title_full_unstemmed All-trans retinoic acid therapy induces asymmetric division of glioma stem cells from the U87MG cell line
title_short All-trans retinoic acid therapy induces asymmetric division of glioma stem cells from the U87MG cell line
title_sort all-trans retinoic acid therapy induces asymmetric division of glioma stem cells from the u87mg cell line
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6757269/
https://www.ncbi.nlm.nih.gov/pubmed/31579077
http://dx.doi.org/10.3892/ol.2019.10691
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