Cargando…
Downregulated miR-21 mediates matrine-induced apoptosis via the PTEN/Akt signaling pathway in FTC-133 human follicular thyroid cancer cells
Matrine is an alkaloid extracted from the leguminous plant Sophora flavescens. Matrine has clinical effects in the treatment of tumors, including those in lung cancer, nasopharyngeal cancer and liver cancer. However, the effect of matrine on follicular thyroid cancer has not been reported. The aim o...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6757298/ https://www.ncbi.nlm.nih.gov/pubmed/31579406 http://dx.doi.org/10.3892/ol.2019.10693 |
_version_ | 1783453552863084544 |
---|---|
author | Li, Quanliang Zhang, Shuya Wang, Mingyu Dong, Shouguang Wang, Yan Liu, Song Lu, Tiancheng Fu, Yuqin Wang, Xiuran Chen, Guihong |
author_facet | Li, Quanliang Zhang, Shuya Wang, Mingyu Dong, Shouguang Wang, Yan Liu, Song Lu, Tiancheng Fu, Yuqin Wang, Xiuran Chen, Guihong |
author_sort | Li, Quanliang |
collection | PubMed |
description | Matrine is an alkaloid extracted from the leguminous plant Sophora flavescens. Matrine has clinical effects in the treatment of tumors, including those in lung cancer, nasopharyngeal cancer and liver cancer. However, the effect of matrine on follicular thyroid cancer has not been reported. The aim of the present study was to investigate the effect of matrine on follicular thyroid cancer and its potential mechanism. FTC-133 follicular thyroid cancer cells were treated with different concentrations of matrine, and an MTT assay showed that matrine inhibited the growth of FTC-133 cells in a dose- and time-dependent manner with an IC(50) value of 154.8 µM. Cell apoptosis was analyzed by flow cytometry and the results showed that matrine effectively induced the apoptosis of FTC-133 cells. The expression level of microRNA (miR)-21 was analyzed by reverse transcription-quantitative PCR (RT-qPCR) analysis, and the mRNA and protein expression levels of PTEN, Akt and phosphorylated (p)-Akt were detected by RT-qPCR analysis and western blotting, respectively. The expression of miR-21 was significantly downregulated, PTEN was upregulated at the mRNA and protein expression levels, and p-Akt was downregulated in the FTC-133 cells. The effects of miR-21 mimics and miR-21 inhibitor on the expression of miR-21, PTEN and Akt in FTC-133 cells, and the effect of miR-21 mimics/matrine on the expression of PTEN were also investigated. The results of the present study suggested that matrine inhibited the growth and induced apoptosis of FTC-133 cells via the miR-21/PTEN/Akt signaling pathway. |
format | Online Article Text |
id | pubmed-6757298 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-67572982019-10-02 Downregulated miR-21 mediates matrine-induced apoptosis via the PTEN/Akt signaling pathway in FTC-133 human follicular thyroid cancer cells Li, Quanliang Zhang, Shuya Wang, Mingyu Dong, Shouguang Wang, Yan Liu, Song Lu, Tiancheng Fu, Yuqin Wang, Xiuran Chen, Guihong Oncol Lett Articles Matrine is an alkaloid extracted from the leguminous plant Sophora flavescens. Matrine has clinical effects in the treatment of tumors, including those in lung cancer, nasopharyngeal cancer and liver cancer. However, the effect of matrine on follicular thyroid cancer has not been reported. The aim of the present study was to investigate the effect of matrine on follicular thyroid cancer and its potential mechanism. FTC-133 follicular thyroid cancer cells were treated with different concentrations of matrine, and an MTT assay showed that matrine inhibited the growth of FTC-133 cells in a dose- and time-dependent manner with an IC(50) value of 154.8 µM. Cell apoptosis was analyzed by flow cytometry and the results showed that matrine effectively induced the apoptosis of FTC-133 cells. The expression level of microRNA (miR)-21 was analyzed by reverse transcription-quantitative PCR (RT-qPCR) analysis, and the mRNA and protein expression levels of PTEN, Akt and phosphorylated (p)-Akt were detected by RT-qPCR analysis and western blotting, respectively. The expression of miR-21 was significantly downregulated, PTEN was upregulated at the mRNA and protein expression levels, and p-Akt was downregulated in the FTC-133 cells. The effects of miR-21 mimics and miR-21 inhibitor on the expression of miR-21, PTEN and Akt in FTC-133 cells, and the effect of miR-21 mimics/matrine on the expression of PTEN were also investigated. The results of the present study suggested that matrine inhibited the growth and induced apoptosis of FTC-133 cells via the miR-21/PTEN/Akt signaling pathway. D.A. Spandidos 2019-10 2019-07-31 /pmc/articles/PMC6757298/ /pubmed/31579406 http://dx.doi.org/10.3892/ol.2019.10693 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Li, Quanliang Zhang, Shuya Wang, Mingyu Dong, Shouguang Wang, Yan Liu, Song Lu, Tiancheng Fu, Yuqin Wang, Xiuran Chen, Guihong Downregulated miR-21 mediates matrine-induced apoptosis via the PTEN/Akt signaling pathway in FTC-133 human follicular thyroid cancer cells |
title | Downregulated miR-21 mediates matrine-induced apoptosis via the PTEN/Akt signaling pathway in FTC-133 human follicular thyroid cancer cells |
title_full | Downregulated miR-21 mediates matrine-induced apoptosis via the PTEN/Akt signaling pathway in FTC-133 human follicular thyroid cancer cells |
title_fullStr | Downregulated miR-21 mediates matrine-induced apoptosis via the PTEN/Akt signaling pathway in FTC-133 human follicular thyroid cancer cells |
title_full_unstemmed | Downregulated miR-21 mediates matrine-induced apoptosis via the PTEN/Akt signaling pathway in FTC-133 human follicular thyroid cancer cells |
title_short | Downregulated miR-21 mediates matrine-induced apoptosis via the PTEN/Akt signaling pathway in FTC-133 human follicular thyroid cancer cells |
title_sort | downregulated mir-21 mediates matrine-induced apoptosis via the pten/akt signaling pathway in ftc-133 human follicular thyroid cancer cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6757298/ https://www.ncbi.nlm.nih.gov/pubmed/31579406 http://dx.doi.org/10.3892/ol.2019.10693 |
work_keys_str_mv | AT liquanliang downregulatedmir21mediatesmatrineinducedapoptosisviatheptenaktsignalingpathwayinftc133humanfollicularthyroidcancercells AT zhangshuya downregulatedmir21mediatesmatrineinducedapoptosisviatheptenaktsignalingpathwayinftc133humanfollicularthyroidcancercells AT wangmingyu downregulatedmir21mediatesmatrineinducedapoptosisviatheptenaktsignalingpathwayinftc133humanfollicularthyroidcancercells AT dongshouguang downregulatedmir21mediatesmatrineinducedapoptosisviatheptenaktsignalingpathwayinftc133humanfollicularthyroidcancercells AT wangyan downregulatedmir21mediatesmatrineinducedapoptosisviatheptenaktsignalingpathwayinftc133humanfollicularthyroidcancercells AT liusong downregulatedmir21mediatesmatrineinducedapoptosisviatheptenaktsignalingpathwayinftc133humanfollicularthyroidcancercells AT lutiancheng downregulatedmir21mediatesmatrineinducedapoptosisviatheptenaktsignalingpathwayinftc133humanfollicularthyroidcancercells AT fuyuqin downregulatedmir21mediatesmatrineinducedapoptosisviatheptenaktsignalingpathwayinftc133humanfollicularthyroidcancercells AT wangxiuran downregulatedmir21mediatesmatrineinducedapoptosisviatheptenaktsignalingpathwayinftc133humanfollicularthyroidcancercells AT chenguihong downregulatedmir21mediatesmatrineinducedapoptosisviatheptenaktsignalingpathwayinftc133humanfollicularthyroidcancercells |