Cargando…
Alpha-Terpineol production from an engineered Saccharomyces cerevisiae cell factory
BACKGROUND: Alpha-Terpineol (α-Terpineol), a C(10) monoterpenoid alcohol, is widely used in the cosmetic and pharmaceutical industries. Construction Saccharomyces cerevisiae cell factories for producing monoterpenes offers a promising means to substitute chemical synthesis or phytoextraction. RESULT...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6757357/ https://www.ncbi.nlm.nih.gov/pubmed/31547812 http://dx.doi.org/10.1186/s12934-019-1211-0 |
_version_ | 1783453559989207040 |
---|---|
author | Zhang, Chuanbo Li, Man Zhao, Guang-Rong Lu, Wenyu |
author_facet | Zhang, Chuanbo Li, Man Zhao, Guang-Rong Lu, Wenyu |
author_sort | Zhang, Chuanbo |
collection | PubMed |
description | BACKGROUND: Alpha-Terpineol (α-Terpineol), a C(10) monoterpenoid alcohol, is widely used in the cosmetic and pharmaceutical industries. Construction Saccharomyces cerevisiae cell factories for producing monoterpenes offers a promising means to substitute chemical synthesis or phytoextraction. RESULTS: α-Terpineol was produced by expressing the truncated α-Terpineol synthase (tVvTS) from Vitis vinifera in S. cerevisiae. The α-Terpineol titer was increased to 0.83 mg/L with overexpression of the rate-limiting genes tHMG1, IDI1 and ERG20(F96W-N127W). A GSGSGSGSGS linker was applied to fuse ERG20(F96W-N127W) with tVvTS, and expressing the fusion protein increased the α-Terpineol production by 2.87-fold to 2.39 mg/L when compared with the parental strain. In addition, we found that farnesyl diphosphate (FPP) accumulation by down-regulation of ERG9 expression and deletion of LPP1 and DPP1 did not improve α-Terpineol production. Therefore, ERG9 was overexpressed and the α-Terpineol titer was further increased to 3.32 mg/L. The best α-Terpineol producing strain LCB08 was then used for batch and fed-batch fermentation in a 5 L bioreactor, and the production of α-Terpineol was ultimately improved to 21.88 mg/L. CONCLUSIONS: An efficient α-Terpineol production cell factory was constructed by engineering the S. cerevisiae mevalonate pathway, and the metabolic engineering strategies could also be applied to produce other valuable monoterpene compounds in yeast. |
format | Online Article Text |
id | pubmed-6757357 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-67573572019-09-30 Alpha-Terpineol production from an engineered Saccharomyces cerevisiae cell factory Zhang, Chuanbo Li, Man Zhao, Guang-Rong Lu, Wenyu Microb Cell Fact Research BACKGROUND: Alpha-Terpineol (α-Terpineol), a C(10) monoterpenoid alcohol, is widely used in the cosmetic and pharmaceutical industries. Construction Saccharomyces cerevisiae cell factories for producing monoterpenes offers a promising means to substitute chemical synthesis or phytoextraction. RESULTS: α-Terpineol was produced by expressing the truncated α-Terpineol synthase (tVvTS) from Vitis vinifera in S. cerevisiae. The α-Terpineol titer was increased to 0.83 mg/L with overexpression of the rate-limiting genes tHMG1, IDI1 and ERG20(F96W-N127W). A GSGSGSGSGS linker was applied to fuse ERG20(F96W-N127W) with tVvTS, and expressing the fusion protein increased the α-Terpineol production by 2.87-fold to 2.39 mg/L when compared with the parental strain. In addition, we found that farnesyl diphosphate (FPP) accumulation by down-regulation of ERG9 expression and deletion of LPP1 and DPP1 did not improve α-Terpineol production. Therefore, ERG9 was overexpressed and the α-Terpineol titer was further increased to 3.32 mg/L. The best α-Terpineol producing strain LCB08 was then used for batch and fed-batch fermentation in a 5 L bioreactor, and the production of α-Terpineol was ultimately improved to 21.88 mg/L. CONCLUSIONS: An efficient α-Terpineol production cell factory was constructed by engineering the S. cerevisiae mevalonate pathway, and the metabolic engineering strategies could also be applied to produce other valuable monoterpene compounds in yeast. BioMed Central 2019-09-23 /pmc/articles/PMC6757357/ /pubmed/31547812 http://dx.doi.org/10.1186/s12934-019-1211-0 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Zhang, Chuanbo Li, Man Zhao, Guang-Rong Lu, Wenyu Alpha-Terpineol production from an engineered Saccharomyces cerevisiae cell factory |
title | Alpha-Terpineol production from an engineered Saccharomyces cerevisiae cell factory |
title_full | Alpha-Terpineol production from an engineered Saccharomyces cerevisiae cell factory |
title_fullStr | Alpha-Terpineol production from an engineered Saccharomyces cerevisiae cell factory |
title_full_unstemmed | Alpha-Terpineol production from an engineered Saccharomyces cerevisiae cell factory |
title_short | Alpha-Terpineol production from an engineered Saccharomyces cerevisiae cell factory |
title_sort | alpha-terpineol production from an engineered saccharomyces cerevisiae cell factory |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6757357/ https://www.ncbi.nlm.nih.gov/pubmed/31547812 http://dx.doi.org/10.1186/s12934-019-1211-0 |
work_keys_str_mv | AT zhangchuanbo alphaterpineolproductionfromanengineeredsaccharomycescerevisiaecellfactory AT liman alphaterpineolproductionfromanengineeredsaccharomycescerevisiaecellfactory AT zhaoguangrong alphaterpineolproductionfromanengineeredsaccharomycescerevisiaecellfactory AT luwenyu alphaterpineolproductionfromanengineeredsaccharomycescerevisiaecellfactory |