Cargando…

Virulence of atypical Toxoplasma gondii strains isolated in French Guiana in a murine model

Background. Toxoplasma gondii is an obligate intracellular protozoan parasite of warm-blooded vertebrates. Most infections in immunocompetent patients are asymptomatic. However, since 2000s, strains with particular genetic profiles that differ from the known clonal type (type I, II, III), have been...

Descripción completa

Detalles Bibliográficos
Autores principales: Simon, Stéphane, de Thoisy, Benoit, Mercier, Aurélien, Nacher, Mathieu, Demar, Magalie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6757855/
https://www.ncbi.nlm.nih.gov/pubmed/31549631
http://dx.doi.org/10.1051/parasite/2019048
_version_ 1783453588424491008
author Simon, Stéphane
de Thoisy, Benoit
Mercier, Aurélien
Nacher, Mathieu
Demar, Magalie
author_facet Simon, Stéphane
de Thoisy, Benoit
Mercier, Aurélien
Nacher, Mathieu
Demar, Magalie
author_sort Simon, Stéphane
collection PubMed
description Background. Toxoplasma gondii is an obligate intracellular protozoan parasite of warm-blooded vertebrates. Most infections in immunocompetent patients are asymptomatic. However, since 2000s, strains with particular genetic profiles that differ from the known clonal type (type I, II, III), have been described. In French Guiana, these strains are highly pathogenic in immunocompetent patients. They have defined a new clinical entity called Amazonian Toxoplasmosis. The present study aims to further improve our knowledge on the pathogenicity of these Amazonian T. gondii strains in comparison with three reference strains using Swiss strain mice. With these data, we tried to establish a predictive virulence score to classify these strains, but also to correlate this virulence with the severity of the disease in infected patients. Results. All the virulence indicators revealed that the Amazonian strains isolated in French Guiana presented a high virulence profile, but lower than the highly virulent type I reference RH strain. The findings reveal differences in virulence between human and animal strains, but also between anthropized and wild strains. Conclusion. In addition to being a clinically relevant animal model of Amazonian Toxoplasmosis, this model could also provide a solid experimental basis for future studies aiming to investigate the underlying mechanisms of Amazonian Toxoplasmosis disease.
format Online
Article
Text
id pubmed-6757855
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher EDP Sciences
record_format MEDLINE/PubMed
spelling pubmed-67578552019-10-02 Virulence of atypical Toxoplasma gondii strains isolated in French Guiana in a murine model Simon, Stéphane de Thoisy, Benoit Mercier, Aurélien Nacher, Mathieu Demar, Magalie Parasite Research Article Background. Toxoplasma gondii is an obligate intracellular protozoan parasite of warm-blooded vertebrates. Most infections in immunocompetent patients are asymptomatic. However, since 2000s, strains with particular genetic profiles that differ from the known clonal type (type I, II, III), have been described. In French Guiana, these strains are highly pathogenic in immunocompetent patients. They have defined a new clinical entity called Amazonian Toxoplasmosis. The present study aims to further improve our knowledge on the pathogenicity of these Amazonian T. gondii strains in comparison with three reference strains using Swiss strain mice. With these data, we tried to establish a predictive virulence score to classify these strains, but also to correlate this virulence with the severity of the disease in infected patients. Results. All the virulence indicators revealed that the Amazonian strains isolated in French Guiana presented a high virulence profile, but lower than the highly virulent type I reference RH strain. The findings reveal differences in virulence between human and animal strains, but also between anthropized and wild strains. Conclusion. In addition to being a clinically relevant animal model of Amazonian Toxoplasmosis, this model could also provide a solid experimental basis for future studies aiming to investigate the underlying mechanisms of Amazonian Toxoplasmosis disease. EDP Sciences 2019-09-24 /pmc/articles/PMC6757855/ /pubmed/31549631 http://dx.doi.org/10.1051/parasite/2019048 Text en © S. Simon et al., published by EDP Sciences, 2019 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Simon, Stéphane
de Thoisy, Benoit
Mercier, Aurélien
Nacher, Mathieu
Demar, Magalie
Virulence of atypical Toxoplasma gondii strains isolated in French Guiana in a murine model
title Virulence of atypical Toxoplasma gondii strains isolated in French Guiana in a murine model
title_full Virulence of atypical Toxoplasma gondii strains isolated in French Guiana in a murine model
title_fullStr Virulence of atypical Toxoplasma gondii strains isolated in French Guiana in a murine model
title_full_unstemmed Virulence of atypical Toxoplasma gondii strains isolated in French Guiana in a murine model
title_short Virulence of atypical Toxoplasma gondii strains isolated in French Guiana in a murine model
title_sort virulence of atypical toxoplasma gondii strains isolated in french guiana in a murine model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6757855/
https://www.ncbi.nlm.nih.gov/pubmed/31549631
http://dx.doi.org/10.1051/parasite/2019048
work_keys_str_mv AT simonstephane virulenceofatypicaltoxoplasmagondiistrainsisolatedinfrenchguianainamurinemodel
AT dethoisybenoit virulenceofatypicaltoxoplasmagondiistrainsisolatedinfrenchguianainamurinemodel
AT mercieraurelien virulenceofatypicaltoxoplasmagondiistrainsisolatedinfrenchguianainamurinemodel
AT nachermathieu virulenceofatypicaltoxoplasmagondiistrainsisolatedinfrenchguianainamurinemodel
AT demarmagalie virulenceofatypicaltoxoplasmagondiistrainsisolatedinfrenchguianainamurinemodel