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Virulence of atypical Toxoplasma gondii strains isolated in French Guiana in a murine model
Background. Toxoplasma gondii is an obligate intracellular protozoan parasite of warm-blooded vertebrates. Most infections in immunocompetent patients are asymptomatic. However, since 2000s, strains with particular genetic profiles that differ from the known clonal type (type I, II, III), have been...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
EDP Sciences
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6757855/ https://www.ncbi.nlm.nih.gov/pubmed/31549631 http://dx.doi.org/10.1051/parasite/2019048 |
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author | Simon, Stéphane de Thoisy, Benoit Mercier, Aurélien Nacher, Mathieu Demar, Magalie |
author_facet | Simon, Stéphane de Thoisy, Benoit Mercier, Aurélien Nacher, Mathieu Demar, Magalie |
author_sort | Simon, Stéphane |
collection | PubMed |
description | Background. Toxoplasma gondii is an obligate intracellular protozoan parasite of warm-blooded vertebrates. Most infections in immunocompetent patients are asymptomatic. However, since 2000s, strains with particular genetic profiles that differ from the known clonal type (type I, II, III), have been described. In French Guiana, these strains are highly pathogenic in immunocompetent patients. They have defined a new clinical entity called Amazonian Toxoplasmosis. The present study aims to further improve our knowledge on the pathogenicity of these Amazonian T. gondii strains in comparison with three reference strains using Swiss strain mice. With these data, we tried to establish a predictive virulence score to classify these strains, but also to correlate this virulence with the severity of the disease in infected patients. Results. All the virulence indicators revealed that the Amazonian strains isolated in French Guiana presented a high virulence profile, but lower than the highly virulent type I reference RH strain. The findings reveal differences in virulence between human and animal strains, but also between anthropized and wild strains. Conclusion. In addition to being a clinically relevant animal model of Amazonian Toxoplasmosis, this model could also provide a solid experimental basis for future studies aiming to investigate the underlying mechanisms of Amazonian Toxoplasmosis disease. |
format | Online Article Text |
id | pubmed-6757855 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | EDP Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-67578552019-10-02 Virulence of atypical Toxoplasma gondii strains isolated in French Guiana in a murine model Simon, Stéphane de Thoisy, Benoit Mercier, Aurélien Nacher, Mathieu Demar, Magalie Parasite Research Article Background. Toxoplasma gondii is an obligate intracellular protozoan parasite of warm-blooded vertebrates. Most infections in immunocompetent patients are asymptomatic. However, since 2000s, strains with particular genetic profiles that differ from the known clonal type (type I, II, III), have been described. In French Guiana, these strains are highly pathogenic in immunocompetent patients. They have defined a new clinical entity called Amazonian Toxoplasmosis. The present study aims to further improve our knowledge on the pathogenicity of these Amazonian T. gondii strains in comparison with three reference strains using Swiss strain mice. With these data, we tried to establish a predictive virulence score to classify these strains, but also to correlate this virulence with the severity of the disease in infected patients. Results. All the virulence indicators revealed that the Amazonian strains isolated in French Guiana presented a high virulence profile, but lower than the highly virulent type I reference RH strain. The findings reveal differences in virulence between human and animal strains, but also between anthropized and wild strains. Conclusion. In addition to being a clinically relevant animal model of Amazonian Toxoplasmosis, this model could also provide a solid experimental basis for future studies aiming to investigate the underlying mechanisms of Amazonian Toxoplasmosis disease. EDP Sciences 2019-09-24 /pmc/articles/PMC6757855/ /pubmed/31549631 http://dx.doi.org/10.1051/parasite/2019048 Text en © S. Simon et al., published by EDP Sciences, 2019 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Simon, Stéphane de Thoisy, Benoit Mercier, Aurélien Nacher, Mathieu Demar, Magalie Virulence of atypical Toxoplasma gondii strains isolated in French Guiana in a murine model |
title | Virulence of atypical Toxoplasma gondii strains isolated in French Guiana in a murine model |
title_full | Virulence of atypical Toxoplasma gondii strains isolated in French Guiana in a murine model |
title_fullStr | Virulence of atypical Toxoplasma gondii strains isolated in French Guiana in a murine model |
title_full_unstemmed | Virulence of atypical Toxoplasma gondii strains isolated in French Guiana in a murine model |
title_short | Virulence of atypical Toxoplasma gondii strains isolated in French Guiana in a murine model |
title_sort | virulence of atypical toxoplasma gondii strains isolated in french guiana in a murine model |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6757855/ https://www.ncbi.nlm.nih.gov/pubmed/31549631 http://dx.doi.org/10.1051/parasite/2019048 |
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