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Phylogeny of Hawaiian Melicope (Rutaceae): RAD-seq Resolves Species Relationships and Reveals Ancient Introgression

Hawaiian Melicope are one of the major adaptive radiations of the Hawaiian Islands comprising 54 endemic species. The lineage is monophyletic with an estimated crown age predating the rise of the current high islands. Phylogenetic inference based on Sanger sequencing has not been sufficient to resol...

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Autores principales: Paetzold, Claudia, Wood, Kenneth R., Eaton, Deren A. R., Wagner, Warren L., Appelhans, Marc S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6758601/
https://www.ncbi.nlm.nih.gov/pubmed/31608076
http://dx.doi.org/10.3389/fpls.2019.01074
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author Paetzold, Claudia
Wood, Kenneth R.
Eaton, Deren A. R.
Wagner, Warren L.
Appelhans, Marc S.
author_facet Paetzold, Claudia
Wood, Kenneth R.
Eaton, Deren A. R.
Wagner, Warren L.
Appelhans, Marc S.
author_sort Paetzold, Claudia
collection PubMed
description Hawaiian Melicope are one of the major adaptive radiations of the Hawaiian Islands comprising 54 endemic species. The lineage is monophyletic with an estimated crown age predating the rise of the current high islands. Phylogenetic inference based on Sanger sequencing has not been sufficient to resolve species or deeper level relationships. Here, we apply restriction site-associated DNA sequencing (RAD-seq) to the lineage to infer phylogenetic relationships. We employ Quartet Sampling to assess information content and statistical support, and to quantify discordance as well as partitioned ABBA-BABA tests to uncover evidence of introgression. Our new results drastically improved resolution of relationships within Hawaiian Melicope. The lineage is divided into five fully supported main clades, two of which correspond to morphologically circumscribed infrageneric groups. We provide evidence for both ancestral and current hybridization events. We confirm the necessity for a taxonomic revision of the Melicope section Pelea, as well as a re-evaluation of several species complexes by combining genomic and morphological data.
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spelling pubmed-67586012019-10-11 Phylogeny of Hawaiian Melicope (Rutaceae): RAD-seq Resolves Species Relationships and Reveals Ancient Introgression Paetzold, Claudia Wood, Kenneth R. Eaton, Deren A. R. Wagner, Warren L. Appelhans, Marc S. Front Plant Sci Plant Science Hawaiian Melicope are one of the major adaptive radiations of the Hawaiian Islands comprising 54 endemic species. The lineage is monophyletic with an estimated crown age predating the rise of the current high islands. Phylogenetic inference based on Sanger sequencing has not been sufficient to resolve species or deeper level relationships. Here, we apply restriction site-associated DNA sequencing (RAD-seq) to the lineage to infer phylogenetic relationships. We employ Quartet Sampling to assess information content and statistical support, and to quantify discordance as well as partitioned ABBA-BABA tests to uncover evidence of introgression. Our new results drastically improved resolution of relationships within Hawaiian Melicope. The lineage is divided into five fully supported main clades, two of which correspond to morphologically circumscribed infrageneric groups. We provide evidence for both ancestral and current hybridization events. We confirm the necessity for a taxonomic revision of the Melicope section Pelea, as well as a re-evaluation of several species complexes by combining genomic and morphological data. Frontiers Media S.A. 2019-09-17 /pmc/articles/PMC6758601/ /pubmed/31608076 http://dx.doi.org/10.3389/fpls.2019.01074 Text en Copyright © 2019 Paetzold, Wood, Eaton, Wagner and Appelhans http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Paetzold, Claudia
Wood, Kenneth R.
Eaton, Deren A. R.
Wagner, Warren L.
Appelhans, Marc S.
Phylogeny of Hawaiian Melicope (Rutaceae): RAD-seq Resolves Species Relationships and Reveals Ancient Introgression
title Phylogeny of Hawaiian Melicope (Rutaceae): RAD-seq Resolves Species Relationships and Reveals Ancient Introgression
title_full Phylogeny of Hawaiian Melicope (Rutaceae): RAD-seq Resolves Species Relationships and Reveals Ancient Introgression
title_fullStr Phylogeny of Hawaiian Melicope (Rutaceae): RAD-seq Resolves Species Relationships and Reveals Ancient Introgression
title_full_unstemmed Phylogeny of Hawaiian Melicope (Rutaceae): RAD-seq Resolves Species Relationships and Reveals Ancient Introgression
title_short Phylogeny of Hawaiian Melicope (Rutaceae): RAD-seq Resolves Species Relationships and Reveals Ancient Introgression
title_sort phylogeny of hawaiian melicope (rutaceae): rad-seq resolves species relationships and reveals ancient introgression
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6758601/
https://www.ncbi.nlm.nih.gov/pubmed/31608076
http://dx.doi.org/10.3389/fpls.2019.01074
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