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De novo transcriptome analysis of Viola ×wittrockiana exposed to high temperature stress
Around the world, pansies are one of the most popular garden flowers, but they are generally sensitive to high temperatures, and this limits the practicality of planting them during the warmest days of the year. However, a few pansy germplasms with improved heat tolerance have been discovered or bre...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6759194/ https://www.ncbi.nlm.nih.gov/pubmed/31550256 http://dx.doi.org/10.1371/journal.pone.0222344 |
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author | Du, Xiaohua Zhu, Xiaopei Yang, Yaping Wang, Yanli Arens, Paul Liu, Huichao |
author_facet | Du, Xiaohua Zhu, Xiaopei Yang, Yaping Wang, Yanli Arens, Paul Liu, Huichao |
author_sort | Du, Xiaohua |
collection | PubMed |
description | Around the world, pansies are one of the most popular garden flowers, but they are generally sensitive to high temperatures, and this limits the practicality of planting them during the warmest days of the year. However, a few pansy germplasms with improved heat tolerance have been discovered or bred, but the mechanisms of their heat resistance are not understood. In this study, we investigated the transcript profiles of a heat-tolerant pansy inbred line, DFM16, in response to high temperatures using RNAseq. Approximately 55.48 Gb of nucleotide data were obtained and assembled into 167,576 unigenes with an average length of 959 bp, of which, 5,708 genes were found to be differentially expressed after heat treatments. Real-time qPCR was performed to validate the expression profiles of the selected genes. Nine metabolic pathways were found to be significantly enriched, in the analysis of the differentially expressed genes. Several potentially interesting genes that encoded putative transcription regulators or key components involving heat shock protein (HSP), heat shock transcription factors (HSF), and antioxidants biosynthesis, were identified. These genes were highlighted to indicate their significance in response to heat stress and will be used as candidate genes to improve pansy heat-tolerance in the future. |
format | Online Article Text |
id | pubmed-6759194 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-67591942019-10-04 De novo transcriptome analysis of Viola ×wittrockiana exposed to high temperature stress Du, Xiaohua Zhu, Xiaopei Yang, Yaping Wang, Yanli Arens, Paul Liu, Huichao PLoS One Research Article Around the world, pansies are one of the most popular garden flowers, but they are generally sensitive to high temperatures, and this limits the practicality of planting them during the warmest days of the year. However, a few pansy germplasms with improved heat tolerance have been discovered or bred, but the mechanisms of their heat resistance are not understood. In this study, we investigated the transcript profiles of a heat-tolerant pansy inbred line, DFM16, in response to high temperatures using RNAseq. Approximately 55.48 Gb of nucleotide data were obtained and assembled into 167,576 unigenes with an average length of 959 bp, of which, 5,708 genes were found to be differentially expressed after heat treatments. Real-time qPCR was performed to validate the expression profiles of the selected genes. Nine metabolic pathways were found to be significantly enriched, in the analysis of the differentially expressed genes. Several potentially interesting genes that encoded putative transcription regulators or key components involving heat shock protein (HSP), heat shock transcription factors (HSF), and antioxidants biosynthesis, were identified. These genes were highlighted to indicate their significance in response to heat stress and will be used as candidate genes to improve pansy heat-tolerance in the future. Public Library of Science 2019-09-24 /pmc/articles/PMC6759194/ /pubmed/31550256 http://dx.doi.org/10.1371/journal.pone.0222344 Text en © 2019 Du et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Du, Xiaohua Zhu, Xiaopei Yang, Yaping Wang, Yanli Arens, Paul Liu, Huichao De novo transcriptome analysis of Viola ×wittrockiana exposed to high temperature stress |
title | De novo transcriptome analysis of Viola ×wittrockiana exposed to high temperature stress |
title_full | De novo transcriptome analysis of Viola ×wittrockiana exposed to high temperature stress |
title_fullStr | De novo transcriptome analysis of Viola ×wittrockiana exposed to high temperature stress |
title_full_unstemmed | De novo transcriptome analysis of Viola ×wittrockiana exposed to high temperature stress |
title_short | De novo transcriptome analysis of Viola ×wittrockiana exposed to high temperature stress |
title_sort | de novo transcriptome analysis of viola ×wittrockiana exposed to high temperature stress |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6759194/ https://www.ncbi.nlm.nih.gov/pubmed/31550256 http://dx.doi.org/10.1371/journal.pone.0222344 |
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