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Sensitive and Specific Detection of Low-Level Antibody Responses in Mild Middle East Respiratory Syndrome Coronavirus Infections

Middle East respiratory syndrome coronavirus (MERS-CoV) infections in humans can cause asymptomatic to fatal lower respiratory lung disease. Despite posing a probable risk for virus transmission, asymptomatic to mild infections can go unnoticed; a lack of seroconversion among some PCR-confirmed case...

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Detalles Bibliográficos
Autores principales: Okba, Nisreen M.A., Raj, V. Stalin, Widjaja, Ivy, GeurtsvanKessel, Corine H., de Bruin, Erwin, Chandler, Felicity D., Park, Wan Beom, Kim, Nam-Joong, Farag, Elmoubasher A.B.A., Al-Hajri, Mohammed, Bosch, Berend-Jan, Oh, Myoung-don, Koopmans, Marion P.G., Reusken, Chantal B.E.M., Haagmans, Bart L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6759241/
https://www.ncbi.nlm.nih.gov/pubmed/31423970
http://dx.doi.org/10.3201/eid2510.190051
Descripción
Sumario:Middle East respiratory syndrome coronavirus (MERS-CoV) infections in humans can cause asymptomatic to fatal lower respiratory lung disease. Despite posing a probable risk for virus transmission, asymptomatic to mild infections can go unnoticed; a lack of seroconversion among some PCR-confirmed cases has been reported. We found that a MERS-CoV spike S1 protein–based ELISA, routinely used in surveillance studies, showed low sensitivity in detecting infections among PCR-confirmed patients with mild clinical symptoms and cross-reactivity of human coronavirus OC43–positive serum samples. Using in-house S1 ELISA and protein microarray, we demonstrate that most PCR-confirmed MERS-CoV case-patients with mild infections seroconverted; nonetheless, some of these samples did not have detectable levels of virus-neutralizing antibodies. The use of a sensitive and specific serologic S1-based assay can be instrumental in the accurate estimation of MERS-CoV prevalence.