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Optimizing bacterial DNA extraction in urine

Urine is an acceptable, non-invasive sample for investigating the human urogenital microbiota and for the diagnosis of sexually transmitted infections. However, low quantities of bacterial DNA and PCR inhibitors in urine may prevent efficient PCR amplification for molecular detection of bacteria. Fu...

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Detalles Bibliográficos
Autores principales: Munch, Matthew M., Chambers, Laura C., Manhart, Lisa E., Domogala, Dan, Lopez, Anthony, Fredricks, David N., Srinivasan, Sujatha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6759279/
https://www.ncbi.nlm.nih.gov/pubmed/31550285
http://dx.doi.org/10.1371/journal.pone.0222962