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Optimizing bacterial DNA extraction in urine
Urine is an acceptable, non-invasive sample for investigating the human urogenital microbiota and for the diagnosis of sexually transmitted infections. However, low quantities of bacterial DNA and PCR inhibitors in urine may prevent efficient PCR amplification for molecular detection of bacteria. Fu...
Autores principales: | Munch, Matthew M., Chambers, Laura C., Manhart, Lisa E., Domogala, Dan, Lopez, Anthony, Fredricks, David N., Srinivasan, Sujatha |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6759279/ https://www.ncbi.nlm.nih.gov/pubmed/31550285 http://dx.doi.org/10.1371/journal.pone.0222962 |
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