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Visualizing trypanosomes in a vertebrate host reveals novel swimming behaviours, adaptations and attachment mechanisms

Trypanosomes are important disease agents of humans, livestock and cold-blooded species, including fish. The cellular morphology of trypanosomes is central to their motility, adaptation to the host’s environments and pathogenesis. However, visualizing the behaviour of trypanosomes resident in a live...

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Autores principales: Dóró, Éva, Jacobs, Sem H, Hammond, Ffion R, Schipper, Henk, Pieters, Remco PM, Carrington, Mark, Wiegertjes, Geert F, Forlenza, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6759355/
https://www.ncbi.nlm.nih.gov/pubmed/31547905
http://dx.doi.org/10.7554/eLife.48388
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author Dóró, Éva
Jacobs, Sem H
Hammond, Ffion R
Schipper, Henk
Pieters, Remco PM
Carrington, Mark
Wiegertjes, Geert F
Forlenza, Maria
author_facet Dóró, Éva
Jacobs, Sem H
Hammond, Ffion R
Schipper, Henk
Pieters, Remco PM
Carrington, Mark
Wiegertjes, Geert F
Forlenza, Maria
author_sort Dóró, Éva
collection PubMed
description Trypanosomes are important disease agents of humans, livestock and cold-blooded species, including fish. The cellular morphology of trypanosomes is central to their motility, adaptation to the host’s environments and pathogenesis. However, visualizing the behaviour of trypanosomes resident in a live vertebrate host has remained unexplored. In this study, we describe an infection model of zebrafish (Danio rerio) with Trypanosoma carassii. By combining high spatio-temporal resolution microscopy with the transparency of live zebrafish, we describe in detail the swimming behaviour of trypanosomes in blood and tissues of a vertebrate host. Besides the conventional tumbling and directional swimming, T. carassii can change direction through a ‘whip-like’ motion or by swimming backward. Further, the posterior end can act as an anchoring site in vivo. To our knowledge, this is the first report of a vertebrate infection model that allows detailed imaging of trypanosome swimming behaviour in vivo in a natural host environment.
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spelling pubmed-67593552019-09-26 Visualizing trypanosomes in a vertebrate host reveals novel swimming behaviours, adaptations and attachment mechanisms Dóró, Éva Jacobs, Sem H Hammond, Ffion R Schipper, Henk Pieters, Remco PM Carrington, Mark Wiegertjes, Geert F Forlenza, Maria eLife Microbiology and Infectious Disease Trypanosomes are important disease agents of humans, livestock and cold-blooded species, including fish. The cellular morphology of trypanosomes is central to their motility, adaptation to the host’s environments and pathogenesis. However, visualizing the behaviour of trypanosomes resident in a live vertebrate host has remained unexplored. In this study, we describe an infection model of zebrafish (Danio rerio) with Trypanosoma carassii. By combining high spatio-temporal resolution microscopy with the transparency of live zebrafish, we describe in detail the swimming behaviour of trypanosomes in blood and tissues of a vertebrate host. Besides the conventional tumbling and directional swimming, T. carassii can change direction through a ‘whip-like’ motion or by swimming backward. Further, the posterior end can act as an anchoring site in vivo. To our knowledge, this is the first report of a vertebrate infection model that allows detailed imaging of trypanosome swimming behaviour in vivo in a natural host environment. eLife Sciences Publications, Ltd 2019-09-24 /pmc/articles/PMC6759355/ /pubmed/31547905 http://dx.doi.org/10.7554/eLife.48388 Text en © 2019, Dóró et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Microbiology and Infectious Disease
Dóró, Éva
Jacobs, Sem H
Hammond, Ffion R
Schipper, Henk
Pieters, Remco PM
Carrington, Mark
Wiegertjes, Geert F
Forlenza, Maria
Visualizing trypanosomes in a vertebrate host reveals novel swimming behaviours, adaptations and attachment mechanisms
title Visualizing trypanosomes in a vertebrate host reveals novel swimming behaviours, adaptations and attachment mechanisms
title_full Visualizing trypanosomes in a vertebrate host reveals novel swimming behaviours, adaptations and attachment mechanisms
title_fullStr Visualizing trypanosomes in a vertebrate host reveals novel swimming behaviours, adaptations and attachment mechanisms
title_full_unstemmed Visualizing trypanosomes in a vertebrate host reveals novel swimming behaviours, adaptations and attachment mechanisms
title_short Visualizing trypanosomes in a vertebrate host reveals novel swimming behaviours, adaptations and attachment mechanisms
title_sort visualizing trypanosomes in a vertebrate host reveals novel swimming behaviours, adaptations and attachment mechanisms
topic Microbiology and Infectious Disease
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6759355/
https://www.ncbi.nlm.nih.gov/pubmed/31547905
http://dx.doi.org/10.7554/eLife.48388
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