Identification of Transgene-Free CRISPR-Edited Plants of Rice, Tomato, and Arabidopsis by Monitoring DsRED Fluorescence in Dry Seeds

Efficient elimination of the editing machinery remains a challenge in plant biotechnology after genome editing to minimize the probability of off-target mutations, but it is also important to deliver end users with edited plants free of foreign DNA. Using the modular cloning system Golden Braid, we...

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Detalles Bibliográficos
Autores principales: Aliaga-Franco, Norma, Zhang, Cunjin, Presa, Silvia, Srivastava, Anjil K., Granell, Antonio, Alabadí, David, Sadanandom, Ari, Blázquez, Miguel A., Minguet, Eugenio G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6759815/
https://www.ncbi.nlm.nih.gov/pubmed/31620160
http://dx.doi.org/10.3389/fpls.2019.01150

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