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Early Neospora caninum infection dynamics in cattle after inoculation at mid-gestation with high (Nc-Spain7)- or low (Nc-Spain1H)-virulence isolates

Early Neospora caninum infection dynamics were investigated in pregnant heifers intravenously inoculated with PBS (G-Control) or 10(7) tachyzoites of high (G-NcSpain7)- or low (G-NcSpain1H)-virulence isolates at 110 days of gestation. Serial culling at 10 and 20 days post-infection (dpi) was perform...

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Autores principales: Jiménez-Pelayo, Laura, García-Sánchez, Marta, Vázquez, Patricia, Regidor-Cerrillo, Javier, Horcajo, Pilar, Collantes-Fernández, Esther, Blanco-Murcia, Javier, Gutiérrez-Expósito, Daniel, Román-Trufero, Alicia, Osoro, Koldo, Benavides, Julio, Ortega-Mora, Luis Miguel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6760050/
https://www.ncbi.nlm.nih.gov/pubmed/31551079
http://dx.doi.org/10.1186/s13567-019-0691-6
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author Jiménez-Pelayo, Laura
García-Sánchez, Marta
Vázquez, Patricia
Regidor-Cerrillo, Javier
Horcajo, Pilar
Collantes-Fernández, Esther
Blanco-Murcia, Javier
Gutiérrez-Expósito, Daniel
Román-Trufero, Alicia
Osoro, Koldo
Benavides, Julio
Ortega-Mora, Luis Miguel
author_facet Jiménez-Pelayo, Laura
García-Sánchez, Marta
Vázquez, Patricia
Regidor-Cerrillo, Javier
Horcajo, Pilar
Collantes-Fernández, Esther
Blanco-Murcia, Javier
Gutiérrez-Expósito, Daniel
Román-Trufero, Alicia
Osoro, Koldo
Benavides, Julio
Ortega-Mora, Luis Miguel
author_sort Jiménez-Pelayo, Laura
collection PubMed
description Early Neospora caninum infection dynamics were investigated in pregnant heifers intravenously inoculated with PBS (G-Control) or 10(7) tachyzoites of high (G-NcSpain7)- or low (G-NcSpain1H)-virulence isolates at 110 days of gestation. Serial culling at 10 and 20 days post-infection (dpi) was performed. Fever was detected at 1 dpi in both infected groups (P < 0.0001), and a second peak was detected at 3 dpi only in G-NcSpain7 (P < 0.0001). At 10 dpi, Nc-Spain7 was detected in placental samples from one animal related to focal necrosis, and Nc-Spain7 transmission was observed, although no foetal lesions were associated with this finding. The presence of Nc-Spain1H in the placenta or foetuses, as well as lesions, were not detected at 10 dpi. At 20 dpi, G-NcSpain7 animals showed almost 100% positive placental tissues and severe focal necrosis as well as 100% transmission. Remarkably, foetal mortality was detected in two G-NcSpain7 heifers. Only one animal from G-NcSpain1H presented positive placental samples. No foetal mortality was detected, and lesions and parasite transmission to the foetus were not observed in this group. Finally, 100% of G-NcSpain7 heifers at 20 dpi presented specific antibodies, while only 60% of G-NcSpain1H animals presented specific antibodies at 20 dpi. In addition, earlier seroconversion in G-Nc-Spain7 was observed. In conclusion, tachyzoites from Nc-Spain7 reached the placenta earlier and multiplied, leading to lesion development, transmission to the foetus and foetal mortality, whereas Nc-Spain1H showed delayed infection of the placenta and no lesional development or transmission during early infection.
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spelling pubmed-67600502019-09-30 Early Neospora caninum infection dynamics in cattle after inoculation at mid-gestation with high (Nc-Spain7)- or low (Nc-Spain1H)-virulence isolates Jiménez-Pelayo, Laura García-Sánchez, Marta Vázquez, Patricia Regidor-Cerrillo, Javier Horcajo, Pilar Collantes-Fernández, Esther Blanco-Murcia, Javier Gutiérrez-Expósito, Daniel Román-Trufero, Alicia Osoro, Koldo Benavides, Julio Ortega-Mora, Luis Miguel Vet Res Research Article Early Neospora caninum infection dynamics were investigated in pregnant heifers intravenously inoculated with PBS (G-Control) or 10(7) tachyzoites of high (G-NcSpain7)- or low (G-NcSpain1H)-virulence isolates at 110 days of gestation. Serial culling at 10 and 20 days post-infection (dpi) was performed. Fever was detected at 1 dpi in both infected groups (P < 0.0001), and a second peak was detected at 3 dpi only in G-NcSpain7 (P < 0.0001). At 10 dpi, Nc-Spain7 was detected in placental samples from one animal related to focal necrosis, and Nc-Spain7 transmission was observed, although no foetal lesions were associated with this finding. The presence of Nc-Spain1H in the placenta or foetuses, as well as lesions, were not detected at 10 dpi. At 20 dpi, G-NcSpain7 animals showed almost 100% positive placental tissues and severe focal necrosis as well as 100% transmission. Remarkably, foetal mortality was detected in two G-NcSpain7 heifers. Only one animal from G-NcSpain1H presented positive placental samples. No foetal mortality was detected, and lesions and parasite transmission to the foetus were not observed in this group. Finally, 100% of G-NcSpain7 heifers at 20 dpi presented specific antibodies, while only 60% of G-NcSpain1H animals presented specific antibodies at 20 dpi. In addition, earlier seroconversion in G-Nc-Spain7 was observed. In conclusion, tachyzoites from Nc-Spain7 reached the placenta earlier and multiplied, leading to lesion development, transmission to the foetus and foetal mortality, whereas Nc-Spain1H showed delayed infection of the placenta and no lesional development or transmission during early infection. BioMed Central 2019-09-24 2019 /pmc/articles/PMC6760050/ /pubmed/31551079 http://dx.doi.org/10.1186/s13567-019-0691-6 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Jiménez-Pelayo, Laura
García-Sánchez, Marta
Vázquez, Patricia
Regidor-Cerrillo, Javier
Horcajo, Pilar
Collantes-Fernández, Esther
Blanco-Murcia, Javier
Gutiérrez-Expósito, Daniel
Román-Trufero, Alicia
Osoro, Koldo
Benavides, Julio
Ortega-Mora, Luis Miguel
Early Neospora caninum infection dynamics in cattle after inoculation at mid-gestation with high (Nc-Spain7)- or low (Nc-Spain1H)-virulence isolates
title Early Neospora caninum infection dynamics in cattle after inoculation at mid-gestation with high (Nc-Spain7)- or low (Nc-Spain1H)-virulence isolates
title_full Early Neospora caninum infection dynamics in cattle after inoculation at mid-gestation with high (Nc-Spain7)- or low (Nc-Spain1H)-virulence isolates
title_fullStr Early Neospora caninum infection dynamics in cattle after inoculation at mid-gestation with high (Nc-Spain7)- or low (Nc-Spain1H)-virulence isolates
title_full_unstemmed Early Neospora caninum infection dynamics in cattle after inoculation at mid-gestation with high (Nc-Spain7)- or low (Nc-Spain1H)-virulence isolates
title_short Early Neospora caninum infection dynamics in cattle after inoculation at mid-gestation with high (Nc-Spain7)- or low (Nc-Spain1H)-virulence isolates
title_sort early neospora caninum infection dynamics in cattle after inoculation at mid-gestation with high (nc-spain7)- or low (nc-spain1h)-virulence isolates
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6760050/
https://www.ncbi.nlm.nih.gov/pubmed/31551079
http://dx.doi.org/10.1186/s13567-019-0691-6
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