Quantitative Phase and Intensity Microscopy Using Snapshot White Light Wavefront Sensing

Phase imaging techniques are an invaluable tool in microscopy for quickly examining thin transparent specimens. Existing methods are limited to either simple and inexpensive methods that produce only qualitative phase information (e.g. phase contrast microscopy, DIC), or significantly more elaborate...

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Autores principales: Wang, Congli, Fu, Qiang, Dun, Xiong, Heidrich, Wolfgang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6760235/
https://www.ncbi.nlm.nih.gov/pubmed/31551461
http://dx.doi.org/10.1038/s41598-019-50264-3
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author Wang, Congli
Fu, Qiang
Dun, Xiong
Heidrich, Wolfgang
author_facet Wang, Congli
Fu, Qiang
Dun, Xiong
Heidrich, Wolfgang
author_sort Wang, Congli
collection PubMed
description Phase imaging techniques are an invaluable tool in microscopy for quickly examining thin transparent specimens. Existing methods are limited to either simple and inexpensive methods that produce only qualitative phase information (e.g. phase contrast microscopy, DIC), or significantly more elaborate and expensive quantitative methods. Here we demonstrate a low-cost, easy to implement microscopy setup for quantitative imaging of phase and bright field amplitude using collimated white light illumination.
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spelling pubmed-67602352019-11-12 Quantitative Phase and Intensity Microscopy Using Snapshot White Light Wavefront Sensing Wang, Congli Fu, Qiang Dun, Xiong Heidrich, Wolfgang Sci Rep Article Phase imaging techniques are an invaluable tool in microscopy for quickly examining thin transparent specimens. Existing methods are limited to either simple and inexpensive methods that produce only qualitative phase information (e.g. phase contrast microscopy, DIC), or significantly more elaborate and expensive quantitative methods. Here we demonstrate a low-cost, easy to implement microscopy setup for quantitative imaging of phase and bright field amplitude using collimated white light illumination. Nature Publishing Group UK 2019-09-24 /pmc/articles/PMC6760235/ /pubmed/31551461 http://dx.doi.org/10.1038/s41598-019-50264-3 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wang, Congli
Fu, Qiang
Dun, Xiong
Heidrich, Wolfgang
Quantitative Phase and Intensity Microscopy Using Snapshot White Light Wavefront Sensing
title Quantitative Phase and Intensity Microscopy Using Snapshot White Light Wavefront Sensing
title_full Quantitative Phase and Intensity Microscopy Using Snapshot White Light Wavefront Sensing
title_fullStr Quantitative Phase and Intensity Microscopy Using Snapshot White Light Wavefront Sensing
title_full_unstemmed Quantitative Phase and Intensity Microscopy Using Snapshot White Light Wavefront Sensing
title_short Quantitative Phase and Intensity Microscopy Using Snapshot White Light Wavefront Sensing
title_sort quantitative phase and intensity microscopy using snapshot white light wavefront sensing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6760235/
https://www.ncbi.nlm.nih.gov/pubmed/31551461
http://dx.doi.org/10.1038/s41598-019-50264-3
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