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Prevalence of antibiotic resistance and integrons, sul and Smqnr genes in clinical isolates of Stenotrophomonas maltophilia from a tertiary care hospital in Southwest Iran

OBJECTIVE(S): Stenotrophomonas maltophilia has emerged as an important opportunistic nosocomial pathogen due to its intrinsic and acquired resistance to a wide range of antimicrobial agents. The present study aimed to investigate the occurrence of antibiotic resistance and resistance mechanisms amon...

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Autores principales: Ebrahim-Saraie, Hadi Sedigh, Heidari, Hamid, Soltani, Behnaz, Mardaneh, Jalal, Motamedifar, Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6760486/
https://www.ncbi.nlm.nih.gov/pubmed/31579442
http://dx.doi.org/10.22038/ijbms.2019.31291.7540
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author Ebrahim-Saraie, Hadi Sedigh
Heidari, Hamid
Soltani, Behnaz
Mardaneh, Jalal
Motamedifar, Mohammad
author_facet Ebrahim-Saraie, Hadi Sedigh
Heidari, Hamid
Soltani, Behnaz
Mardaneh, Jalal
Motamedifar, Mohammad
author_sort Ebrahim-Saraie, Hadi Sedigh
collection PubMed
description OBJECTIVE(S): Stenotrophomonas maltophilia has emerged as an important opportunistic nosocomial pathogen due to its intrinsic and acquired resistance to a wide range of antimicrobial agents. The present study aimed to investigate the occurrence of antibiotic resistance and resistance mechanisms among clinical isolates of S. maltophilia from Iranian patients. MATERIALS AND METHODS: This cross-sectional study was performed on 44 S. maltophilia isolates that were recovered from different clinical specimens in 2015 and 2016. Conventional microbiologic methods were used for primary identification of isolates and confirmed by specific polymerase chain reaction (PCR) primers. Minimum inhibitory concentrations (MICs) were determined by the E-test. PCR was applied to determine antibiotic resistance genes. RESULTS: All of S. maltophilia isolates were susceptible to trimethoprim/sulfamethoxazole (TMP/SMX) and colistin. Moreover, the susceptibility rates of isolates toward ceftazidime and ciprofloxacin were 93.2%, and 84.1%, respectively. Class 1 integrons was detected in 24 (54.5%) isolates by the presence of int1 gene. Moreover, the prevalence of antibiotic resistance genes sul1, sul2, and Smqnr were found in 16 (36.4%), 15 (34.1%), and 29 (65.9%) isolates, respectively. CONCLUSION: In summary, the prevalence of sul and Smqnr genes in integrons-contained isolates point out the significant risk of sulfonamides and fluoroquinolones resistance among clinical isolates of S. maltophilia in our region.
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spelling pubmed-67604862019-10-02 Prevalence of antibiotic resistance and integrons, sul and Smqnr genes in clinical isolates of Stenotrophomonas maltophilia from a tertiary care hospital in Southwest Iran Ebrahim-Saraie, Hadi Sedigh Heidari, Hamid Soltani, Behnaz Mardaneh, Jalal Motamedifar, Mohammad Iran J Basic Med Sci Original Article OBJECTIVE(S): Stenotrophomonas maltophilia has emerged as an important opportunistic nosocomial pathogen due to its intrinsic and acquired resistance to a wide range of antimicrobial agents. The present study aimed to investigate the occurrence of antibiotic resistance and resistance mechanisms among clinical isolates of S. maltophilia from Iranian patients. MATERIALS AND METHODS: This cross-sectional study was performed on 44 S. maltophilia isolates that were recovered from different clinical specimens in 2015 and 2016. Conventional microbiologic methods were used for primary identification of isolates and confirmed by specific polymerase chain reaction (PCR) primers. Minimum inhibitory concentrations (MICs) were determined by the E-test. PCR was applied to determine antibiotic resistance genes. RESULTS: All of S. maltophilia isolates were susceptible to trimethoprim/sulfamethoxazole (TMP/SMX) and colistin. Moreover, the susceptibility rates of isolates toward ceftazidime and ciprofloxacin were 93.2%, and 84.1%, respectively. Class 1 integrons was detected in 24 (54.5%) isolates by the presence of int1 gene. Moreover, the prevalence of antibiotic resistance genes sul1, sul2, and Smqnr were found in 16 (36.4%), 15 (34.1%), and 29 (65.9%) isolates, respectively. CONCLUSION: In summary, the prevalence of sul and Smqnr genes in integrons-contained isolates point out the significant risk of sulfonamides and fluoroquinolones resistance among clinical isolates of S. maltophilia in our region. Mashhad University of Medical Sciences 2019-08 /pmc/articles/PMC6760486/ /pubmed/31579442 http://dx.doi.org/10.22038/ijbms.2019.31291.7540 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Ebrahim-Saraie, Hadi Sedigh
Heidari, Hamid
Soltani, Behnaz
Mardaneh, Jalal
Motamedifar, Mohammad
Prevalence of antibiotic resistance and integrons, sul and Smqnr genes in clinical isolates of Stenotrophomonas maltophilia from a tertiary care hospital in Southwest Iran
title Prevalence of antibiotic resistance and integrons, sul and Smqnr genes in clinical isolates of Stenotrophomonas maltophilia from a tertiary care hospital in Southwest Iran
title_full Prevalence of antibiotic resistance and integrons, sul and Smqnr genes in clinical isolates of Stenotrophomonas maltophilia from a tertiary care hospital in Southwest Iran
title_fullStr Prevalence of antibiotic resistance and integrons, sul and Smqnr genes in clinical isolates of Stenotrophomonas maltophilia from a tertiary care hospital in Southwest Iran
title_full_unstemmed Prevalence of antibiotic resistance and integrons, sul and Smqnr genes in clinical isolates of Stenotrophomonas maltophilia from a tertiary care hospital in Southwest Iran
title_short Prevalence of antibiotic resistance and integrons, sul and Smqnr genes in clinical isolates of Stenotrophomonas maltophilia from a tertiary care hospital in Southwest Iran
title_sort prevalence of antibiotic resistance and integrons, sul and smqnr genes in clinical isolates of stenotrophomonas maltophilia from a tertiary care hospital in southwest iran
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6760486/
https://www.ncbi.nlm.nih.gov/pubmed/31579442
http://dx.doi.org/10.22038/ijbms.2019.31291.7540
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