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Immunogenicity and protection effects of cationic liposome containing imiquimod adjuvant on leishmaniasis in BALB/c mice

OBJECTIVE(S): Protection against leishmaniasis, in the murine model, is dependent on developing a potent CD4(+) mediated Th1 type response. Liposomes can be applied as immunoadjuvants to stimulate immune responses to different antigens. In the present study, it was investigated whether DOTAP liposom...

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Autores principales: Mehravaran, Ahmad, Rezaei Nasab, Maryam, Mirahmadi, Hadi, Sharifi, Iraj, Alijani, Ebrahim, Nikpoor, Amin Reza, Akhtari, Javad, Hojatizade, Mansure
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6760488/
https://www.ncbi.nlm.nih.gov/pubmed/31579449
http://dx.doi.org/10.22038/ijbms.2019.35739.8515
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author Mehravaran, Ahmad
Rezaei Nasab, Maryam
Mirahmadi, Hadi
Sharifi, Iraj
Alijani, Ebrahim
Nikpoor, Amin Reza
Akhtari, Javad
Hojatizade, Mansure
author_facet Mehravaran, Ahmad
Rezaei Nasab, Maryam
Mirahmadi, Hadi
Sharifi, Iraj
Alijani, Ebrahim
Nikpoor, Amin Reza
Akhtari, Javad
Hojatizade, Mansure
author_sort Mehravaran, Ahmad
collection PubMed
description OBJECTIVE(S): Protection against leishmaniasis, in the murine model, is dependent on developing a potent CD4(+) mediated Th1 type response. Liposomes can be applied as immunoadjuvants to stimulate immune responses to different antigens. In the present study, it was investigated whether DOTAP liposomes having SLA and imiquimod adjuvant, can induce a Th1 response and protect against Leishmania major challenge in BALB/c mice. MATERIALS AND METHODS: Liposomes were provided applying the lipid film procedure. BALB/C mice were subcutaneously immunized, three times with 2-week intervals, with various formulations. Assessment of lesion development and parasite burden in the foot and spleen after challenge with L. major, assessment of Th1 cytokine (IFN-γ), and titration of IgG isotypes assessed the type of generated immune reaction and the protection extent. RESULTS: The mice immunized with Liposome DOTAP+imiquimod+SLA showed smaller footpad swelling which was meaningfully different (P<0.05) compared with other groups. The highest level of IgG2a was observed with Lip DOTAP+imiquimod+SLA more than the control (P<0.001). Mice immunized with Lip DOTAP+SLA+imiquimod demonstrated the least number of live parasites in the footpad and spleen. Cytokine assay showed that the greatest IFN- γ secretion was seen in the splenocytes of mice immunized with all formulations as compared to the control group (P<0.0001). In contrast, the lowest IL-4 production was detectable in Lip+imiquimod+SLA spleen, which was not significantly different compared with other groups. CONCLUSION: The results of this study show that liposome DOTAP+SLA+imiquimod formulation generates a cellular immune response that is protective against challenge against L. major.
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spelling pubmed-67604882019-10-02 Immunogenicity and protection effects of cationic liposome containing imiquimod adjuvant on leishmaniasis in BALB/c mice Mehravaran, Ahmad Rezaei Nasab, Maryam Mirahmadi, Hadi Sharifi, Iraj Alijani, Ebrahim Nikpoor, Amin Reza Akhtari, Javad Hojatizade, Mansure Iran J Basic Med Sci Original Article OBJECTIVE(S): Protection against leishmaniasis, in the murine model, is dependent on developing a potent CD4(+) mediated Th1 type response. Liposomes can be applied as immunoadjuvants to stimulate immune responses to different antigens. In the present study, it was investigated whether DOTAP liposomes having SLA and imiquimod adjuvant, can induce a Th1 response and protect against Leishmania major challenge in BALB/c mice. MATERIALS AND METHODS: Liposomes were provided applying the lipid film procedure. BALB/C mice were subcutaneously immunized, three times with 2-week intervals, with various formulations. Assessment of lesion development and parasite burden in the foot and spleen after challenge with L. major, assessment of Th1 cytokine (IFN-γ), and titration of IgG isotypes assessed the type of generated immune reaction and the protection extent. RESULTS: The mice immunized with Liposome DOTAP+imiquimod+SLA showed smaller footpad swelling which was meaningfully different (P<0.05) compared with other groups. The highest level of IgG2a was observed with Lip DOTAP+imiquimod+SLA more than the control (P<0.001). Mice immunized with Lip DOTAP+SLA+imiquimod demonstrated the least number of live parasites in the footpad and spleen. Cytokine assay showed that the greatest IFN- γ secretion was seen in the splenocytes of mice immunized with all formulations as compared to the control group (P<0.0001). In contrast, the lowest IL-4 production was detectable in Lip+imiquimod+SLA spleen, which was not significantly different compared with other groups. CONCLUSION: The results of this study show that liposome DOTAP+SLA+imiquimod formulation generates a cellular immune response that is protective against challenge against L. major. Mashhad University of Medical Sciences 2019-08 /pmc/articles/PMC6760488/ /pubmed/31579449 http://dx.doi.org/10.22038/ijbms.2019.35739.8515 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Mehravaran, Ahmad
Rezaei Nasab, Maryam
Mirahmadi, Hadi
Sharifi, Iraj
Alijani, Ebrahim
Nikpoor, Amin Reza
Akhtari, Javad
Hojatizade, Mansure
Immunogenicity and protection effects of cationic liposome containing imiquimod adjuvant on leishmaniasis in BALB/c mice
title Immunogenicity and protection effects of cationic liposome containing imiquimod adjuvant on leishmaniasis in BALB/c mice
title_full Immunogenicity and protection effects of cationic liposome containing imiquimod adjuvant on leishmaniasis in BALB/c mice
title_fullStr Immunogenicity and protection effects of cationic liposome containing imiquimod adjuvant on leishmaniasis in BALB/c mice
title_full_unstemmed Immunogenicity and protection effects of cationic liposome containing imiquimod adjuvant on leishmaniasis in BALB/c mice
title_short Immunogenicity and protection effects of cationic liposome containing imiquimod adjuvant on leishmaniasis in BALB/c mice
title_sort immunogenicity and protection effects of cationic liposome containing imiquimod adjuvant on leishmaniasis in balb/c mice
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6760488/
https://www.ncbi.nlm.nih.gov/pubmed/31579449
http://dx.doi.org/10.22038/ijbms.2019.35739.8515
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