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Inhibition effect of flavophospholipol on conjugative transfer of the extended-spectrum β-lactamase and vanA genes

Flavophospholipol (FPL) is an antimicrobial feed additive that has been approved for use in livestock animals and has the potential to decrease horizontal dissemination of antimicrobial resistance genes. Since previous studies showed that FPL has an inhibitory effect on plasmid transfer, in vitro ex...

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Autores principales: Kudo, Hayami, Usui, Masaru, Nagafuji, Wataru, Oka, Kentaro, Takahashi, Motomichi, Yamaguchi, Hiroyuki, Tamura, Yutaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6760635/
https://www.ncbi.nlm.nih.gov/pubmed/30361635
http://dx.doi.org/10.1038/s41429-018-0113-4
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author Kudo, Hayami
Usui, Masaru
Nagafuji, Wataru
Oka, Kentaro
Takahashi, Motomichi
Yamaguchi, Hiroyuki
Tamura, Yutaka
author_facet Kudo, Hayami
Usui, Masaru
Nagafuji, Wataru
Oka, Kentaro
Takahashi, Motomichi
Yamaguchi, Hiroyuki
Tamura, Yutaka
author_sort Kudo, Hayami
collection PubMed
description Flavophospholipol (FPL) is an antimicrobial feed additive that has been approved for use in livestock animals and has the potential to decrease horizontal dissemination of antimicrobial resistance genes. Since previous studies showed that FPL has an inhibitory effect on plasmid transfer, in vitro experiments have proven the efficacy of FPL in reducing the conjugative transfer of plasmids encoding the extended-spectrum β-lactamase (ESBL) and vanA genes. These are among the most important antimicrobial resistance loci known. ESBL-producing Escherichia coli and vancomycin-resistant Enterococcus faecalis (VRE) were exposed to several concentrations of FPL, and transfer frequency and plasmid curing activity were determined. FPL inhibited the conjugative transfer of plasmids harboring ESBL and vanA genes in a concentration-dependent manner in all strains. Further transfer experiments revealed that FPL could decrease or increase transfer frequency depending on plasmid type when transfer frequency was at low levels. The plasmid curing activity of FPL was also observed in ESBL-producing E. coli in a concentration-dependent manner, suggesting that they partially contribute to the inhibition of conjugative transfer. These results suggest that the use of FPL as a feed additive might decrease the dissemination of ESBL and vanA genes among livestock animals.
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spelling pubmed-67606352019-09-26 Inhibition effect of flavophospholipol on conjugative transfer of the extended-spectrum β-lactamase and vanA genes Kudo, Hayami Usui, Masaru Nagafuji, Wataru Oka, Kentaro Takahashi, Motomichi Yamaguchi, Hiroyuki Tamura, Yutaka J Antibiot (Tokyo) Article Flavophospholipol (FPL) is an antimicrobial feed additive that has been approved for use in livestock animals and has the potential to decrease horizontal dissemination of antimicrobial resistance genes. Since previous studies showed that FPL has an inhibitory effect on plasmid transfer, in vitro experiments have proven the efficacy of FPL in reducing the conjugative transfer of plasmids encoding the extended-spectrum β-lactamase (ESBL) and vanA genes. These are among the most important antimicrobial resistance loci known. ESBL-producing Escherichia coli and vancomycin-resistant Enterococcus faecalis (VRE) were exposed to several concentrations of FPL, and transfer frequency and plasmid curing activity were determined. FPL inhibited the conjugative transfer of plasmids harboring ESBL and vanA genes in a concentration-dependent manner in all strains. Further transfer experiments revealed that FPL could decrease or increase transfer frequency depending on plasmid type when transfer frequency was at low levels. The plasmid curing activity of FPL was also observed in ESBL-producing E. coli in a concentration-dependent manner, suggesting that they partially contribute to the inhibition of conjugative transfer. These results suggest that the use of FPL as a feed additive might decrease the dissemination of ESBL and vanA genes among livestock animals. Nature Publishing Group UK 2018-10-25 2019 /pmc/articles/PMC6760635/ /pubmed/30361635 http://dx.doi.org/10.1038/s41429-018-0113-4 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kudo, Hayami
Usui, Masaru
Nagafuji, Wataru
Oka, Kentaro
Takahashi, Motomichi
Yamaguchi, Hiroyuki
Tamura, Yutaka
Inhibition effect of flavophospholipol on conjugative transfer of the extended-spectrum β-lactamase and vanA genes
title Inhibition effect of flavophospholipol on conjugative transfer of the extended-spectrum β-lactamase and vanA genes
title_full Inhibition effect of flavophospholipol on conjugative transfer of the extended-spectrum β-lactamase and vanA genes
title_fullStr Inhibition effect of flavophospholipol on conjugative transfer of the extended-spectrum β-lactamase and vanA genes
title_full_unstemmed Inhibition effect of flavophospholipol on conjugative transfer of the extended-spectrum β-lactamase and vanA genes
title_short Inhibition effect of flavophospholipol on conjugative transfer of the extended-spectrum β-lactamase and vanA genes
title_sort inhibition effect of flavophospholipol on conjugative transfer of the extended-spectrum β-lactamase and vana genes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6760635/
https://www.ncbi.nlm.nih.gov/pubmed/30361635
http://dx.doi.org/10.1038/s41429-018-0113-4
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