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Molecular characterization of measles virus strains circulating in Cameroon during the 2013-2016 epidemics

The first genotyping data on measles virus (MeV) strains in Cameroon dates from 1994, while other studies were realized in 2001 and 2011 with the establishment of MeV virological surveillance. However, the genetic data of MeV strains circulating in Cameroon remains fragmented and concentrated in cer...

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Autores principales: Obam Mekanda, Franck-Martin, Monamele, Chavely Gwladys, Simo Nemg, Frédy Brice, Yonga, Gilde Martial, Ouapi, Diane, Penlap Beng, Véronique, Batéjat, Christophe, Caro, Valérie, Manuguerra, Jean-Claude, Demanou, Maurice
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6760898/
https://www.ncbi.nlm.nih.gov/pubmed/31553766
http://dx.doi.org/10.1371/journal.pone.0222428
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author Obam Mekanda, Franck-Martin
Monamele, Chavely Gwladys
Simo Nemg, Frédy Brice
Yonga, Gilde Martial
Ouapi, Diane
Penlap Beng, Véronique
Batéjat, Christophe
Caro, Valérie
Manuguerra, Jean-Claude
Demanou, Maurice
author_facet Obam Mekanda, Franck-Martin
Monamele, Chavely Gwladys
Simo Nemg, Frédy Brice
Yonga, Gilde Martial
Ouapi, Diane
Penlap Beng, Véronique
Batéjat, Christophe
Caro, Valérie
Manuguerra, Jean-Claude
Demanou, Maurice
author_sort Obam Mekanda, Franck-Martin
collection PubMed
description The first genotyping data on measles virus (MeV) strains in Cameroon dates from 1994, while other studies were realized in 2001 and 2011 with the establishment of MeV virological surveillance. However, the genetic data of MeV strains circulating in Cameroon remains fragmented and concentrated in certain regions, hence the need for an update. The objective of this study was to have recent data on MeV genotypes circulating in Cameroon. Ninety throat swabs collected during recent measles outbreaks were analyzed by MeV genotyping RT-PCR using the nucleoprotein gene N. The resulting sequences were analyzed on the basis of 450 nucleotides with MEGA 7 software. Overall genome analysis was performed on 40/90 sequences. The strains were from all ten regions and all belonged to cluster 1 of genotype B3. The genotype B3 has been circulating in Cameroon for long periods of time; efforts must be made in immunization for its elimination.
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spelling pubmed-67608982019-10-04 Molecular characterization of measles virus strains circulating in Cameroon during the 2013-2016 epidemics Obam Mekanda, Franck-Martin Monamele, Chavely Gwladys Simo Nemg, Frédy Brice Yonga, Gilde Martial Ouapi, Diane Penlap Beng, Véronique Batéjat, Christophe Caro, Valérie Manuguerra, Jean-Claude Demanou, Maurice PLoS One Research Article The first genotyping data on measles virus (MeV) strains in Cameroon dates from 1994, while other studies were realized in 2001 and 2011 with the establishment of MeV virological surveillance. However, the genetic data of MeV strains circulating in Cameroon remains fragmented and concentrated in certain regions, hence the need for an update. The objective of this study was to have recent data on MeV genotypes circulating in Cameroon. Ninety throat swabs collected during recent measles outbreaks were analyzed by MeV genotyping RT-PCR using the nucleoprotein gene N. The resulting sequences were analyzed on the basis of 450 nucleotides with MEGA 7 software. Overall genome analysis was performed on 40/90 sequences. The strains were from all ten regions and all belonged to cluster 1 of genotype B3. The genotype B3 has been circulating in Cameroon for long periods of time; efforts must be made in immunization for its elimination. Public Library of Science 2019-09-25 /pmc/articles/PMC6760898/ /pubmed/31553766 http://dx.doi.org/10.1371/journal.pone.0222428 Text en © 2019 Obam Mekanda et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Obam Mekanda, Franck-Martin
Monamele, Chavely Gwladys
Simo Nemg, Frédy Brice
Yonga, Gilde Martial
Ouapi, Diane
Penlap Beng, Véronique
Batéjat, Christophe
Caro, Valérie
Manuguerra, Jean-Claude
Demanou, Maurice
Molecular characterization of measles virus strains circulating in Cameroon during the 2013-2016 epidemics
title Molecular characterization of measles virus strains circulating in Cameroon during the 2013-2016 epidemics
title_full Molecular characterization of measles virus strains circulating in Cameroon during the 2013-2016 epidemics
title_fullStr Molecular characterization of measles virus strains circulating in Cameroon during the 2013-2016 epidemics
title_full_unstemmed Molecular characterization of measles virus strains circulating in Cameroon during the 2013-2016 epidemics
title_short Molecular characterization of measles virus strains circulating in Cameroon during the 2013-2016 epidemics
title_sort molecular characterization of measles virus strains circulating in cameroon during the 2013-2016 epidemics
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6760898/
https://www.ncbi.nlm.nih.gov/pubmed/31553766
http://dx.doi.org/10.1371/journal.pone.0222428
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