Transient Mitomycin C-treatment of human corneal epithelial cells and fibroblasts alters cell migration, cytokine secretion, and matrix accumulation

A single application of Mitomycin C (MMC) is used clinically in ophthalmology to reduce scarring and enhance wound resolution after surgery. Here we show in vitro that a 3-hour MMC treatment of primary and telomerase immortalized human corneal limbal epithelial (HCLE) cells impacts their migration a...

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Autores principales: Pal-Ghosh, Sonali, Tadvalkar, Gauri, Lieberman, Verna Rose, Guo, Xiaoqing, Zieske, James D., Hutcheon, Audrey, Stepp, Mary Ann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761181/
https://www.ncbi.nlm.nih.gov/pubmed/31554858
http://dx.doi.org/10.1038/s41598-019-50307-9
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author Pal-Ghosh, Sonali
Tadvalkar, Gauri
Lieberman, Verna Rose
Guo, Xiaoqing
Zieske, James D.
Hutcheon, Audrey
Stepp, Mary Ann
author_facet Pal-Ghosh, Sonali
Tadvalkar, Gauri
Lieberman, Verna Rose
Guo, Xiaoqing
Zieske, James D.
Hutcheon, Audrey
Stepp, Mary Ann
author_sort Pal-Ghosh, Sonali
collection PubMed
description A single application of Mitomycin C (MMC) is used clinically in ophthalmology to reduce scarring and enhance wound resolution after surgery. Here we show in vitro that a 3-hour MMC treatment of primary and telomerase immortalized human corneal limbal epithelial (HCLE) cells impacts their migration and adhesion. Transient MMC treatment induces HCLE expression of senescence associated secretory factors, cytokine secretion, and deposition of laminin 332 for several days. Transient MMC treatment also reduces migration and deposition of transforming growth factor-β1 (TGFβ1)-stimulated collagen by corneal fibroblasts. Using conditioned media from control and MMC treated cells, we demonstrate that factors secreted by MMC-treated corneal epithelial cells attenuate collagen deposition by HCFs whereas those secreted by MMC-treated HCFs do not. These studies are the first to probe the roles played by corneal epithelial cells in reducing collagen deposition by corneal fibroblasts in response to MMC.
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spelling pubmed-67611812019-11-12 Transient Mitomycin C-treatment of human corneal epithelial cells and fibroblasts alters cell migration, cytokine secretion, and matrix accumulation Pal-Ghosh, Sonali Tadvalkar, Gauri Lieberman, Verna Rose Guo, Xiaoqing Zieske, James D. Hutcheon, Audrey Stepp, Mary Ann Sci Rep Article A single application of Mitomycin C (MMC) is used clinically in ophthalmology to reduce scarring and enhance wound resolution after surgery. Here we show in vitro that a 3-hour MMC treatment of primary and telomerase immortalized human corneal limbal epithelial (HCLE) cells impacts their migration and adhesion. Transient MMC treatment induces HCLE expression of senescence associated secretory factors, cytokine secretion, and deposition of laminin 332 for several days. Transient MMC treatment also reduces migration and deposition of transforming growth factor-β1 (TGFβ1)-stimulated collagen by corneal fibroblasts. Using conditioned media from control and MMC treated cells, we demonstrate that factors secreted by MMC-treated corneal epithelial cells attenuate collagen deposition by HCFs whereas those secreted by MMC-treated HCFs do not. These studies are the first to probe the roles played by corneal epithelial cells in reducing collagen deposition by corneal fibroblasts in response to MMC. Nature Publishing Group UK 2019-09-25 /pmc/articles/PMC6761181/ /pubmed/31554858 http://dx.doi.org/10.1038/s41598-019-50307-9 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Pal-Ghosh, Sonali
Tadvalkar, Gauri
Lieberman, Verna Rose
Guo, Xiaoqing
Zieske, James D.
Hutcheon, Audrey
Stepp, Mary Ann
Transient Mitomycin C-treatment of human corneal epithelial cells and fibroblasts alters cell migration, cytokine secretion, and matrix accumulation
title Transient Mitomycin C-treatment of human corneal epithelial cells and fibroblasts alters cell migration, cytokine secretion, and matrix accumulation
title_full Transient Mitomycin C-treatment of human corneal epithelial cells and fibroblasts alters cell migration, cytokine secretion, and matrix accumulation
title_fullStr Transient Mitomycin C-treatment of human corneal epithelial cells and fibroblasts alters cell migration, cytokine secretion, and matrix accumulation
title_full_unstemmed Transient Mitomycin C-treatment of human corneal epithelial cells and fibroblasts alters cell migration, cytokine secretion, and matrix accumulation
title_short Transient Mitomycin C-treatment of human corneal epithelial cells and fibroblasts alters cell migration, cytokine secretion, and matrix accumulation
title_sort transient mitomycin c-treatment of human corneal epithelial cells and fibroblasts alters cell migration, cytokine secretion, and matrix accumulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761181/
https://www.ncbi.nlm.nih.gov/pubmed/31554858
http://dx.doi.org/10.1038/s41598-019-50307-9
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