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Spatiotemporal material functionalization via competitive supramolecular complexation of avidin and biotin analogs
Spatiotemporal control over engineered tissues is highly desirable for various biomedical applications as it emulates the dynamic behavior of natural tissues. Current spatiotemporal biomaterial functionalization approaches are based on cytotoxic, technically challenging, or non-scalable chemistries,...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761202/ https://www.ncbi.nlm.nih.gov/pubmed/31554812 http://dx.doi.org/10.1038/s41467-019-12390-4 |
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author | Kamperman, Tom Koerselman, Michelle Kelder, Cindy Hendriks, Jan Crispim, João F. de Peuter, Xandra Dijkstra, Pieter J. Karperien, Marcel Leijten, Jeroen |
author_facet | Kamperman, Tom Koerselman, Michelle Kelder, Cindy Hendriks, Jan Crispim, João F. de Peuter, Xandra Dijkstra, Pieter J. Karperien, Marcel Leijten, Jeroen |
author_sort | Kamperman, Tom |
collection | PubMed |
description | Spatiotemporal control over engineered tissues is highly desirable for various biomedical applications as it emulates the dynamic behavior of natural tissues. Current spatiotemporal biomaterial functionalization approaches are based on cytotoxic, technically challenging, or non-scalable chemistries, which has hampered their widespread usage. Here we report a strategy to spatiotemporally functionalize (bio)materials based on competitive supramolecular complexation of avidin and biotin analogs. Specifically, an injectable hydrogel is orthogonally post-functionalized with desthiobiotinylated moieties using multivalent neutravidin. In situ exchange of desthiobiotin by biotin enables spatiotemporal material functionalization as demonstrated by the formation of long-range, conformal, and contra-directional biochemical gradients within complex-shaped 3D hydrogels. Temporal control over engineered tissue biochemistry is further demonstrated by timed presentation and sequestration of growth factors using desthiobiotinylated antibodies. The method’s universality is confirmed by modifying hydrogels with biotinylated fluorophores, peptides, nanoparticles, enzymes, and antibodies. Overall, this work provides a facile, cytocompatible, and universal strategy to spatiotemporally functionalize materials. |
format | Online Article Text |
id | pubmed-6761202 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-67612022019-09-27 Spatiotemporal material functionalization via competitive supramolecular complexation of avidin and biotin analogs Kamperman, Tom Koerselman, Michelle Kelder, Cindy Hendriks, Jan Crispim, João F. de Peuter, Xandra Dijkstra, Pieter J. Karperien, Marcel Leijten, Jeroen Nat Commun Article Spatiotemporal control over engineered tissues is highly desirable for various biomedical applications as it emulates the dynamic behavior of natural tissues. Current spatiotemporal biomaterial functionalization approaches are based on cytotoxic, technically challenging, or non-scalable chemistries, which has hampered their widespread usage. Here we report a strategy to spatiotemporally functionalize (bio)materials based on competitive supramolecular complexation of avidin and biotin analogs. Specifically, an injectable hydrogel is orthogonally post-functionalized with desthiobiotinylated moieties using multivalent neutravidin. In situ exchange of desthiobiotin by biotin enables spatiotemporal material functionalization as demonstrated by the formation of long-range, conformal, and contra-directional biochemical gradients within complex-shaped 3D hydrogels. Temporal control over engineered tissue biochemistry is further demonstrated by timed presentation and sequestration of growth factors using desthiobiotinylated antibodies. The method’s universality is confirmed by modifying hydrogels with biotinylated fluorophores, peptides, nanoparticles, enzymes, and antibodies. Overall, this work provides a facile, cytocompatible, and universal strategy to spatiotemporally functionalize materials. Nature Publishing Group UK 2019-09-25 /pmc/articles/PMC6761202/ /pubmed/31554812 http://dx.doi.org/10.1038/s41467-019-12390-4 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Kamperman, Tom Koerselman, Michelle Kelder, Cindy Hendriks, Jan Crispim, João F. de Peuter, Xandra Dijkstra, Pieter J. Karperien, Marcel Leijten, Jeroen Spatiotemporal material functionalization via competitive supramolecular complexation of avidin and biotin analogs |
title | Spatiotemporal material functionalization via competitive supramolecular complexation of avidin and biotin analogs |
title_full | Spatiotemporal material functionalization via competitive supramolecular complexation of avidin and biotin analogs |
title_fullStr | Spatiotemporal material functionalization via competitive supramolecular complexation of avidin and biotin analogs |
title_full_unstemmed | Spatiotemporal material functionalization via competitive supramolecular complexation of avidin and biotin analogs |
title_short | Spatiotemporal material functionalization via competitive supramolecular complexation of avidin and biotin analogs |
title_sort | spatiotemporal material functionalization via competitive supramolecular complexation of avidin and biotin analogs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761202/ https://www.ncbi.nlm.nih.gov/pubmed/31554812 http://dx.doi.org/10.1038/s41467-019-12390-4 |
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