Cargando…
Identification and Characterization of the Phosphate-Solubilizing Bacterium Pantoea sp. S32 in Reclamation Soil in Shanxi, China
Phosphate solubilizing bacteria (PSB) can convert insoluble forms of phosphorus (P) to accessible forms. Five highly efficient PSB strains, H22, Y11, Y14, Y34, and S32, were screened and isolated from an alfalfa rhizosphere in heavy metal-contaminated reclamation area in Shanxi Province, China. Base...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761231/ https://www.ncbi.nlm.nih.gov/pubmed/31608027 http://dx.doi.org/10.3389/fmicb.2019.02171 |
_version_ | 1783453984039632896 |
---|---|
author | Chen, Qian Liu, Shanjiang |
author_facet | Chen, Qian Liu, Shanjiang |
author_sort | Chen, Qian |
collection | PubMed |
description | Phosphate solubilizing bacteria (PSB) can convert insoluble forms of phosphorus (P) to accessible forms. Five highly efficient PSB strains, H22, Y11, Y14, Y34, and S32, were screened and isolated from an alfalfa rhizosphere in heavy metal-contaminated reclamation area in Shanxi Province, China. Based on morphological observations, 16S rRNA sequencing, cellular fatty acid composition analysis, and the BIOLOG test, H22, Y11, and Y34 were identified as Pseudomonas sp., while Y14 and S32 were identified as Pantoea sp. Among them, S32 showed the highest P-solubilizing efficiency in culture medium containing Ca(3)(PO(4))(2), lecithin, and powered phosphate rock. The culture medium conditions to obtain the highest P-solubilization efficiency were optimized as follows: the culture temperature was 30°C; the incubation time was 5 days; the initial pH was 7.0; and glucose served as the carbon source. Furthermore, the P-solubilization efficiency of S32 in media containing CaHPO(4), lecithin, phosphate rock (PR), FePO(4), or AlPO(4) was determined to be 18.38, 3.07, 0.16, 0.51, or 2.62%, respectively. In addition, the acid and alkali phosphatase activities of S32 were tested as 6.94 U/100 mL and 4.12 U/100 mL, respectively. The soil inoculation experiment indicated that inoculation with S32 resulted in an obvious improvement in the available P of both the experimental and reclaimed soil. The rice seedling growth experiment also suggested that the application of S32 significantly increased the plant height, biomass, root growth, and P uptake of rice in both experimental and reclaimed soil. Taken together, the isolated S32 strain showed high P-solubilization capacity for both Pi and Po, and its ameliorative effect on reclaimed soil recovery provides the theoretical basis for crop development in the reclaimed soil of mine field. |
format | Online Article Text |
id | pubmed-6761231 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-67612312019-10-13 Identification and Characterization of the Phosphate-Solubilizing Bacterium Pantoea sp. S32 in Reclamation Soil in Shanxi, China Chen, Qian Liu, Shanjiang Front Microbiol Microbiology Phosphate solubilizing bacteria (PSB) can convert insoluble forms of phosphorus (P) to accessible forms. Five highly efficient PSB strains, H22, Y11, Y14, Y34, and S32, were screened and isolated from an alfalfa rhizosphere in heavy metal-contaminated reclamation area in Shanxi Province, China. Based on morphological observations, 16S rRNA sequencing, cellular fatty acid composition analysis, and the BIOLOG test, H22, Y11, and Y34 were identified as Pseudomonas sp., while Y14 and S32 were identified as Pantoea sp. Among them, S32 showed the highest P-solubilizing efficiency in culture medium containing Ca(3)(PO(4))(2), lecithin, and powered phosphate rock. The culture medium conditions to obtain the highest P-solubilization efficiency were optimized as follows: the culture temperature was 30°C; the incubation time was 5 days; the initial pH was 7.0; and glucose served as the carbon source. Furthermore, the P-solubilization efficiency of S32 in media containing CaHPO(4), lecithin, phosphate rock (PR), FePO(4), or AlPO(4) was determined to be 18.38, 3.07, 0.16, 0.51, or 2.62%, respectively. In addition, the acid and alkali phosphatase activities of S32 were tested as 6.94 U/100 mL and 4.12 U/100 mL, respectively. The soil inoculation experiment indicated that inoculation with S32 resulted in an obvious improvement in the available P of both the experimental and reclaimed soil. The rice seedling growth experiment also suggested that the application of S32 significantly increased the plant height, biomass, root growth, and P uptake of rice in both experimental and reclaimed soil. Taken together, the isolated S32 strain showed high P-solubilization capacity for both Pi and Po, and its ameliorative effect on reclaimed soil recovery provides the theoretical basis for crop development in the reclaimed soil of mine field. Frontiers Media S.A. 2019-09-19 /pmc/articles/PMC6761231/ /pubmed/31608027 http://dx.doi.org/10.3389/fmicb.2019.02171 Text en Copyright © 2019 Chen and Liu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Chen, Qian Liu, Shanjiang Identification and Characterization of the Phosphate-Solubilizing Bacterium Pantoea sp. S32 in Reclamation Soil in Shanxi, China |
title | Identification and Characterization of the Phosphate-Solubilizing Bacterium Pantoea sp. S32 in Reclamation Soil in Shanxi, China |
title_full | Identification and Characterization of the Phosphate-Solubilizing Bacterium Pantoea sp. S32 in Reclamation Soil in Shanxi, China |
title_fullStr | Identification and Characterization of the Phosphate-Solubilizing Bacterium Pantoea sp. S32 in Reclamation Soil in Shanxi, China |
title_full_unstemmed | Identification and Characterization of the Phosphate-Solubilizing Bacterium Pantoea sp. S32 in Reclamation Soil in Shanxi, China |
title_short | Identification and Characterization of the Phosphate-Solubilizing Bacterium Pantoea sp. S32 in Reclamation Soil in Shanxi, China |
title_sort | identification and characterization of the phosphate-solubilizing bacterium pantoea sp. s32 in reclamation soil in shanxi, china |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761231/ https://www.ncbi.nlm.nih.gov/pubmed/31608027 http://dx.doi.org/10.3389/fmicb.2019.02171 |
work_keys_str_mv | AT chenqian identificationandcharacterizationofthephosphatesolubilizingbacteriumpantoeasps32inreclamationsoilinshanxichina AT liushanjiang identificationandcharacterizationofthephosphatesolubilizingbacteriumpantoeasps32inreclamationsoilinshanxichina |