Stool sampling and DNA isolation kits affect DNA quality and bacterial composition following 16S rRNA gene sequencing using MiSeq Illumina platform

Many studies correlate changes in human gut microbiome with the onset of various diseases, mostly by 16S rRNA gene sequencing. Setting up the optimal sampling and DNA isolation procedures is crucial for robustness and reproducibility of the results. We performed a systematic comparison of several sa...

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Detalles Bibliográficos
Autores principales: Videnska, Petra, Smerkova, Kristyna, Zwinsova, Barbora, Popovici, Vlad, Micenkova, Lenka, Sedlar, Karel, Budinska, Eva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761292/
https://www.ncbi.nlm.nih.gov/pubmed/31554833
http://dx.doi.org/10.1038/s41598-019-49520-3

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