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Effects of Cell Differentiation on the Phagocytic Activities of IgM(+) B Cells in a Teleost Fish

Teleost B cells have phagocytic activities for ingesting particulate antigens, such as bacteria, in addition to the functional secretion of immunoglobulins (Igs). In the present study, the phagocytic activities of IgM(+) B cells under various differentiational conditions residing in peripheral blood...

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Detalles Bibliográficos
Autores principales: Wu, Liting, Kong, Linghe, Yang, Yanjian, Bian, Xia, Wu, Siwei, Li, Bingxi, Yin, Xiaoxue, Mu, Liangliang, Li, Jun, Ye, Jianmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761302/
https://www.ncbi.nlm.nih.gov/pubmed/31608055
http://dx.doi.org/10.3389/fimmu.2019.02225
Descripción
Sumario:Teleost B cells have phagocytic activities for ingesting particulate antigens, such as bacteria, in addition to the functional secretion of immunoglobulins (Igs). In the present study, the phagocytic activities of IgM(+) B cells under various differentiational conditions residing in peripheral blood leukocytes were investigated in a teleost fish Nile tilapia (Oreochromis niloticus). The IgM(+) B cells were recognized as IgM(lo) or IgM(hi) subsets based on their membrane IgM (mIgM) levels. The mIgM, secreted IgM (sIgM), major histocompatibility complex class II and reactive oxygen species were detected. Expressions of transcription factors (Pax5 and Blimp-1) and B cell signaling molecules (CD79a, CD79b, BLNK, and LYN) suggested that IgM(lo) B cells were resembling as plasma-like cells and IgM(hi) resembling as naïve/mature B cells, respectively. Analysis of phagocytic activities demonstrated that both IgM(lo) and IgM(hi) B cells have a similar phagocytic ability (phagocytosis percentage); however, the phagocytic capacity [phagocytic index and the mean fluorescence intensity (MFI)] of IgM(hi) B cells was significantly higher than that of IgM(lo) B cells. Taken together, the results indicated that B cell differentiation may cause the decrease of phagocytic capacity but not phagocytic ability of phagocytic IgM(+) B cells in teleost. The finding may provide an evolutionary evidence for understanding the greater specialization of the B cell in more sophisticated adaptive humoral immunity, by decreasing phagocytic activity in order to contribute its function more specifically into antibody-secreting.