Cargando…
SVCT2 Promotes Neural Stem/Progenitor Cells Migration Through Activating CDC42 After Ischemic Stroke
Ischemic stroke is one of the most leading diseases causing death/long-term disability worldwide. Activating endogenous neural stem/progenitors cells (NSPCs), lining in the subventricular zone (SVZ) and dentate gyrus, facilitates injured brain tissue recovery in both short and long-term experimental...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761321/ https://www.ncbi.nlm.nih.gov/pubmed/31607868 http://dx.doi.org/10.3389/fncel.2019.00429 |
_version_ | 1783454004989132800 |
---|---|
author | Yang, Yang Zhang, Kaiyuan Chen, Xuezhu Wang, Ju Lei, Xuejiao Zhong, Jun Xian, Jishu Quan, Yulian Lu, Yongling Huang, Qianying Chen, Jingyu Ge, Hongfei Feng, Hua |
author_facet | Yang, Yang Zhang, Kaiyuan Chen, Xuezhu Wang, Ju Lei, Xuejiao Zhong, Jun Xian, Jishu Quan, Yulian Lu, Yongling Huang, Qianying Chen, Jingyu Ge, Hongfei Feng, Hua |
author_sort | Yang, Yang |
collection | PubMed |
description | Ischemic stroke is one of the most leading diseases causing death/long-term disability worldwide. Activating endogenous neural stem/progenitors cells (NSPCs), lining in the subventricular zone (SVZ) and dentate gyrus, facilitates injured brain tissue recovery in both short and long-term experimental settings. While, only a few proliferated NSPCs migrate toward the lesions to enhance endogenous repair after ischemia. Here, the results indicated that the functional recovery was evidently improved and the infarct volume was significantly reduced with ascorbic acid (AA) treatment in a dose-dependent manner from 125 to 500 mg/Kg, and the suitable therapeutic concentration was 250 mg/Kg. The possible mechanism might be due to activating sodium-vitamin C cotransporter 2 (SVCT2), which was down-regulated in SVZ after ischemia. Furthermore, immunostaining images depicted the number of migrated NSPCs from SVZ were significantly increased with 250 mg/Kg AA treatment or SVCT2 overexpression under the physiological and pathological condition in vivo. Besides, the data also represented that 250 mg/Kg AA or SVCT2 overexpression facilitated NSPCs migration via promoting F-actin assembling in the manner of up-regulating CDC42 expression using oxygen-glucose deprivation in vitro. Collectively, the present study indicates that SVCT2 promotes NSPCs migration through CDC42 activation to facilitate F-actin assembling, which enlarges the therapeutic scope of AA and the role of SVCT2 in NSPCs migration after brain injury. |
format | Online Article Text |
id | pubmed-6761321 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-67613212019-10-13 SVCT2 Promotes Neural Stem/Progenitor Cells Migration Through Activating CDC42 After Ischemic Stroke Yang, Yang Zhang, Kaiyuan Chen, Xuezhu Wang, Ju Lei, Xuejiao Zhong, Jun Xian, Jishu Quan, Yulian Lu, Yongling Huang, Qianying Chen, Jingyu Ge, Hongfei Feng, Hua Front Cell Neurosci Neuroscience Ischemic stroke is one of the most leading diseases causing death/long-term disability worldwide. Activating endogenous neural stem/progenitors cells (NSPCs), lining in the subventricular zone (SVZ) and dentate gyrus, facilitates injured brain tissue recovery in both short and long-term experimental settings. While, only a few proliferated NSPCs migrate toward the lesions to enhance endogenous repair after ischemia. Here, the results indicated that the functional recovery was evidently improved and the infarct volume was significantly reduced with ascorbic acid (AA) treatment in a dose-dependent manner from 125 to 500 mg/Kg, and the suitable therapeutic concentration was 250 mg/Kg. The possible mechanism might be due to activating sodium-vitamin C cotransporter 2 (SVCT2), which was down-regulated in SVZ after ischemia. Furthermore, immunostaining images depicted the number of migrated NSPCs from SVZ were significantly increased with 250 mg/Kg AA treatment or SVCT2 overexpression under the physiological and pathological condition in vivo. Besides, the data also represented that 250 mg/Kg AA or SVCT2 overexpression facilitated NSPCs migration via promoting F-actin assembling in the manner of up-regulating CDC42 expression using oxygen-glucose deprivation in vitro. Collectively, the present study indicates that SVCT2 promotes NSPCs migration through CDC42 activation to facilitate F-actin assembling, which enlarges the therapeutic scope of AA and the role of SVCT2 in NSPCs migration after brain injury. Frontiers Media S.A. 2019-09-19 /pmc/articles/PMC6761321/ /pubmed/31607868 http://dx.doi.org/10.3389/fncel.2019.00429 Text en Copyright © 2019 Yang, Zhang, Chen, Wang, Lei, Zhong, Xian, Quan, Lu, Huang, Chen, Ge and Feng. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Yang, Yang Zhang, Kaiyuan Chen, Xuezhu Wang, Ju Lei, Xuejiao Zhong, Jun Xian, Jishu Quan, Yulian Lu, Yongling Huang, Qianying Chen, Jingyu Ge, Hongfei Feng, Hua SVCT2 Promotes Neural Stem/Progenitor Cells Migration Through Activating CDC42 After Ischemic Stroke |
title | SVCT2 Promotes Neural Stem/Progenitor Cells Migration Through Activating CDC42 After Ischemic Stroke |
title_full | SVCT2 Promotes Neural Stem/Progenitor Cells Migration Through Activating CDC42 After Ischemic Stroke |
title_fullStr | SVCT2 Promotes Neural Stem/Progenitor Cells Migration Through Activating CDC42 After Ischemic Stroke |
title_full_unstemmed | SVCT2 Promotes Neural Stem/Progenitor Cells Migration Through Activating CDC42 After Ischemic Stroke |
title_short | SVCT2 Promotes Neural Stem/Progenitor Cells Migration Through Activating CDC42 After Ischemic Stroke |
title_sort | svct2 promotes neural stem/progenitor cells migration through activating cdc42 after ischemic stroke |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761321/ https://www.ncbi.nlm.nih.gov/pubmed/31607868 http://dx.doi.org/10.3389/fncel.2019.00429 |
work_keys_str_mv | AT yangyang svct2promotesneuralstemprogenitorcellsmigrationthroughactivatingcdc42afterischemicstroke AT zhangkaiyuan svct2promotesneuralstemprogenitorcellsmigrationthroughactivatingcdc42afterischemicstroke AT chenxuezhu svct2promotesneuralstemprogenitorcellsmigrationthroughactivatingcdc42afterischemicstroke AT wangju svct2promotesneuralstemprogenitorcellsmigrationthroughactivatingcdc42afterischemicstroke AT leixuejiao svct2promotesneuralstemprogenitorcellsmigrationthroughactivatingcdc42afterischemicstroke AT zhongjun svct2promotesneuralstemprogenitorcellsmigrationthroughactivatingcdc42afterischemicstroke AT xianjishu svct2promotesneuralstemprogenitorcellsmigrationthroughactivatingcdc42afterischemicstroke AT quanyulian svct2promotesneuralstemprogenitorcellsmigrationthroughactivatingcdc42afterischemicstroke AT luyongling svct2promotesneuralstemprogenitorcellsmigrationthroughactivatingcdc42afterischemicstroke AT huangqianying svct2promotesneuralstemprogenitorcellsmigrationthroughactivatingcdc42afterischemicstroke AT chenjingyu svct2promotesneuralstemprogenitorcellsmigrationthroughactivatingcdc42afterischemicstroke AT gehongfei svct2promotesneuralstemprogenitorcellsmigrationthroughactivatingcdc42afterischemicstroke AT fenghua svct2promotesneuralstemprogenitorcellsmigrationthroughactivatingcdc42afterischemicstroke |