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SVCT2 Promotes Neural Stem/Progenitor Cells Migration Through Activating CDC42 After Ischemic Stroke

Ischemic stroke is one of the most leading diseases causing death/long-term disability worldwide. Activating endogenous neural stem/progenitors cells (NSPCs), lining in the subventricular zone (SVZ) and dentate gyrus, facilitates injured brain tissue recovery in both short and long-term experimental...

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Autores principales: Yang, Yang, Zhang, Kaiyuan, Chen, Xuezhu, Wang, Ju, Lei, Xuejiao, Zhong, Jun, Xian, Jishu, Quan, Yulian, Lu, Yongling, Huang, Qianying, Chen, Jingyu, Ge, Hongfei, Feng, Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761321/
https://www.ncbi.nlm.nih.gov/pubmed/31607868
http://dx.doi.org/10.3389/fncel.2019.00429
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author Yang, Yang
Zhang, Kaiyuan
Chen, Xuezhu
Wang, Ju
Lei, Xuejiao
Zhong, Jun
Xian, Jishu
Quan, Yulian
Lu, Yongling
Huang, Qianying
Chen, Jingyu
Ge, Hongfei
Feng, Hua
author_facet Yang, Yang
Zhang, Kaiyuan
Chen, Xuezhu
Wang, Ju
Lei, Xuejiao
Zhong, Jun
Xian, Jishu
Quan, Yulian
Lu, Yongling
Huang, Qianying
Chen, Jingyu
Ge, Hongfei
Feng, Hua
author_sort Yang, Yang
collection PubMed
description Ischemic stroke is one of the most leading diseases causing death/long-term disability worldwide. Activating endogenous neural stem/progenitors cells (NSPCs), lining in the subventricular zone (SVZ) and dentate gyrus, facilitates injured brain tissue recovery in both short and long-term experimental settings. While, only a few proliferated NSPCs migrate toward the lesions to enhance endogenous repair after ischemia. Here, the results indicated that the functional recovery was evidently improved and the infarct volume was significantly reduced with ascorbic acid (AA) treatment in a dose-dependent manner from 125 to 500 mg/Kg, and the suitable therapeutic concentration was 250 mg/Kg. The possible mechanism might be due to activating sodium-vitamin C cotransporter 2 (SVCT2), which was down-regulated in SVZ after ischemia. Furthermore, immunostaining images depicted the number of migrated NSPCs from SVZ were significantly increased with 250 mg/Kg AA treatment or SVCT2 overexpression under the physiological and pathological condition in vivo. Besides, the data also represented that 250 mg/Kg AA or SVCT2 overexpression facilitated NSPCs migration via promoting F-actin assembling in the manner of up-regulating CDC42 expression using oxygen-glucose deprivation in vitro. Collectively, the present study indicates that SVCT2 promotes NSPCs migration through CDC42 activation to facilitate F-actin assembling, which enlarges the therapeutic scope of AA and the role of SVCT2 in NSPCs migration after brain injury.
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spelling pubmed-67613212019-10-13 SVCT2 Promotes Neural Stem/Progenitor Cells Migration Through Activating CDC42 After Ischemic Stroke Yang, Yang Zhang, Kaiyuan Chen, Xuezhu Wang, Ju Lei, Xuejiao Zhong, Jun Xian, Jishu Quan, Yulian Lu, Yongling Huang, Qianying Chen, Jingyu Ge, Hongfei Feng, Hua Front Cell Neurosci Neuroscience Ischemic stroke is one of the most leading diseases causing death/long-term disability worldwide. Activating endogenous neural stem/progenitors cells (NSPCs), lining in the subventricular zone (SVZ) and dentate gyrus, facilitates injured brain tissue recovery in both short and long-term experimental settings. While, only a few proliferated NSPCs migrate toward the lesions to enhance endogenous repair after ischemia. Here, the results indicated that the functional recovery was evidently improved and the infarct volume was significantly reduced with ascorbic acid (AA) treatment in a dose-dependent manner from 125 to 500 mg/Kg, and the suitable therapeutic concentration was 250 mg/Kg. The possible mechanism might be due to activating sodium-vitamin C cotransporter 2 (SVCT2), which was down-regulated in SVZ after ischemia. Furthermore, immunostaining images depicted the number of migrated NSPCs from SVZ were significantly increased with 250 mg/Kg AA treatment or SVCT2 overexpression under the physiological and pathological condition in vivo. Besides, the data also represented that 250 mg/Kg AA or SVCT2 overexpression facilitated NSPCs migration via promoting F-actin assembling in the manner of up-regulating CDC42 expression using oxygen-glucose deprivation in vitro. Collectively, the present study indicates that SVCT2 promotes NSPCs migration through CDC42 activation to facilitate F-actin assembling, which enlarges the therapeutic scope of AA and the role of SVCT2 in NSPCs migration after brain injury. Frontiers Media S.A. 2019-09-19 /pmc/articles/PMC6761321/ /pubmed/31607868 http://dx.doi.org/10.3389/fncel.2019.00429 Text en Copyright © 2019 Yang, Zhang, Chen, Wang, Lei, Zhong, Xian, Quan, Lu, Huang, Chen, Ge and Feng. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Yang, Yang
Zhang, Kaiyuan
Chen, Xuezhu
Wang, Ju
Lei, Xuejiao
Zhong, Jun
Xian, Jishu
Quan, Yulian
Lu, Yongling
Huang, Qianying
Chen, Jingyu
Ge, Hongfei
Feng, Hua
SVCT2 Promotes Neural Stem/Progenitor Cells Migration Through Activating CDC42 After Ischemic Stroke
title SVCT2 Promotes Neural Stem/Progenitor Cells Migration Through Activating CDC42 After Ischemic Stroke
title_full SVCT2 Promotes Neural Stem/Progenitor Cells Migration Through Activating CDC42 After Ischemic Stroke
title_fullStr SVCT2 Promotes Neural Stem/Progenitor Cells Migration Through Activating CDC42 After Ischemic Stroke
title_full_unstemmed SVCT2 Promotes Neural Stem/Progenitor Cells Migration Through Activating CDC42 After Ischemic Stroke
title_short SVCT2 Promotes Neural Stem/Progenitor Cells Migration Through Activating CDC42 After Ischemic Stroke
title_sort svct2 promotes neural stem/progenitor cells migration through activating cdc42 after ischemic stroke
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761321/
https://www.ncbi.nlm.nih.gov/pubmed/31607868
http://dx.doi.org/10.3389/fncel.2019.00429
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