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Capillary blood as an alternative specimen for enumeration of percentages of lymphocyte subsets

OBJECTIVE: Capillary blood has been increasingly used in point-of-care setting for clinical monitoring in immunology and infectious diseases. We explored whether percentages of lymphocyte subsets (T-cells; CD3+, helper T-cells; CD4+, cytotoxic T-cells; CD8+, B-cells; CD19+, NK cells; CD56+, gamma de...

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Autores principales: Srisala, Supanart, Pongsakul, Nutkridta, Sahakijpicharn, Thiantip, Hongeng, Suradej, Chutipongtanate, Somchai, Apiwattanakul, Nopporn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761722/
https://www.ncbi.nlm.nih.gov/pubmed/31554512
http://dx.doi.org/10.1186/s13104-019-4659-4
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author Srisala, Supanart
Pongsakul, Nutkridta
Sahakijpicharn, Thiantip
Hongeng, Suradej
Chutipongtanate, Somchai
Apiwattanakul, Nopporn
author_facet Srisala, Supanart
Pongsakul, Nutkridta
Sahakijpicharn, Thiantip
Hongeng, Suradej
Chutipongtanate, Somchai
Apiwattanakul, Nopporn
author_sort Srisala, Supanart
collection PubMed
description OBJECTIVE: Capillary blood has been increasingly used in point-of-care setting for clinical monitoring in immunology and infectious diseases. We explored whether percentages of lymphocyte subsets (T-cells; CD3+, helper T-cells; CD4+, cytotoxic T-cells; CD8+, B-cells; CD19+, NK cells; CD56+, gamma delta T-cells, and regulatory T-cells) with regard to total lymphocyte count from capillary and venous blood of healthy volunteers were in good agreement. RESULTS: All percentages of lymphocyte subsets with regard to total lymphocyte count from capillary blood were significantly correlated with those from venous blood (r ≥ 0.9 for every cell type). However, Bland–Altman plots showed high agreement between capillary and venous samples only in those of CD3+, CD4+, and CD8+ cells (limit of agreement percentages from mean venous blood < 20%). However, the agreement of percentages of other lymphocyte subsets from venous and capillary blood was mediocre. We concluded that capillary blood could be used as an alternative for venous blood to determine percentages of CD3+, CD4+, and CD8+ cells with regard to total lymphocyte count.
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spelling pubmed-67617222019-09-30 Capillary blood as an alternative specimen for enumeration of percentages of lymphocyte subsets Srisala, Supanart Pongsakul, Nutkridta Sahakijpicharn, Thiantip Hongeng, Suradej Chutipongtanate, Somchai Apiwattanakul, Nopporn BMC Res Notes Research Note OBJECTIVE: Capillary blood has been increasingly used in point-of-care setting for clinical monitoring in immunology and infectious diseases. We explored whether percentages of lymphocyte subsets (T-cells; CD3+, helper T-cells; CD4+, cytotoxic T-cells; CD8+, B-cells; CD19+, NK cells; CD56+, gamma delta T-cells, and regulatory T-cells) with regard to total lymphocyte count from capillary and venous blood of healthy volunteers were in good agreement. RESULTS: All percentages of lymphocyte subsets with regard to total lymphocyte count from capillary blood were significantly correlated with those from venous blood (r ≥ 0.9 for every cell type). However, Bland–Altman plots showed high agreement between capillary and venous samples only in those of CD3+, CD4+, and CD8+ cells (limit of agreement percentages from mean venous blood < 20%). However, the agreement of percentages of other lymphocyte subsets from venous and capillary blood was mediocre. We concluded that capillary blood could be used as an alternative for venous blood to determine percentages of CD3+, CD4+, and CD8+ cells with regard to total lymphocyte count. BioMed Central 2019-09-26 /pmc/articles/PMC6761722/ /pubmed/31554512 http://dx.doi.org/10.1186/s13104-019-4659-4 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Srisala, Supanart
Pongsakul, Nutkridta
Sahakijpicharn, Thiantip
Hongeng, Suradej
Chutipongtanate, Somchai
Apiwattanakul, Nopporn
Capillary blood as an alternative specimen for enumeration of percentages of lymphocyte subsets
title Capillary blood as an alternative specimen for enumeration of percentages of lymphocyte subsets
title_full Capillary blood as an alternative specimen for enumeration of percentages of lymphocyte subsets
title_fullStr Capillary blood as an alternative specimen for enumeration of percentages of lymphocyte subsets
title_full_unstemmed Capillary blood as an alternative specimen for enumeration of percentages of lymphocyte subsets
title_short Capillary blood as an alternative specimen for enumeration of percentages of lymphocyte subsets
title_sort capillary blood as an alternative specimen for enumeration of percentages of lymphocyte subsets
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761722/
https://www.ncbi.nlm.nih.gov/pubmed/31554512
http://dx.doi.org/10.1186/s13104-019-4659-4
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